MPPE1 Antibody - #DF15972

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Product: MPPE1 Antibody
Catalog: DF15972
Description: Rabbit polyclonal antibody to MPPE1
Application: IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 45kD(Calculated).
Uniprot: Q53F39

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
MPPE1 Antibody detects endogenous levels of MPPE1.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 3.1.-.-; Metallo phosphoesterase; Metallophosphoesterase 1; PGAP5; Post-GPI attachment to proteins factor 5;

Immunogens

Immunogen:

A synthesized peptide derived from human MPPE1.

Uniprot:

Q53F39(MPPE1_HUMAN) >>Visit HPA database.

Gene(ID):
MPPE1(65258)
Expression:
Q53F39 MPPE1_HUMAN:

Expressed in brain.

Sequence:
MAMIELGFGRQNFHPLKRKSSLLLKLIAVVFAVLLFCEFLIYYLAIFQCNWPEVKTTASDGEQTTREPVLKAMFLADTHLLGEFLGHWLDKLRREWQMERAFQTALWLLQPEVVFILGDIFDEGKWSTPEAWADDVERFQKMFRHPSHVQLKVVAGNHDIGFHYEMNTYKVERFEKVFSSERLFSWKGINFVMVNSVALNGDGCGICSETEAELIEVSHRLNCSREARGSSRCGPGPLLPTSAPVLLQHYPLYRRSDANCSGEDAAPAEERDIPFKENYDVLSREASQKLLWWLQPRLVLSGHTHSACEVHHGGRVPELSVPSFSWRNRNNPSFIMGSITPTDYTLSKCYLPREDVVLIIYCGVVGFLVVLTLTHFGLLASPFLSGLNLLGKRKTR

Research Backgrounds

Function:

Metallophosphoesterase required for transport of GPI-anchor proteins from the endoplasmic reticulum to the Golgi. Acts in lipid remodeling steps of GPI-anchor maturation by mediating the removal of a side-chain ethanolamine-phosphate (EtNP) from the second Man (Man2) of the GPI intermediate, an essential step for efficient transport of GPI-anchor proteins.

Subcellular Location:

Endoplasmic reticulum-Golgi intermediate compartment membrane>Multi-pass membrane protein. Golgi apparatus>cis-Golgi network membrane>Multi-pass membrane protein.
Note: Also localizes to endoplasmic reticulum exit site.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain.

Subunit Structure:

Interacts with GPI-anchor proteins. Interacts with TMED10.

Family&Domains:

The di-lysine motif (KxKxx) acts as an endoplasmic reticulum retrieval signal.

Belongs to the metallophosphoesterase superfamily. MPPE1 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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