Product: AurB Antibody
Catalog: AF6475
Description: Rabbit polyclonal antibody to AurB
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 39kDa; 39kD(Calculated).
Uniprot: Q96GD4
RRID: AB_2835294

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 100ul $280 In stock
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Product Info

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
AurB Antibody detects endogenous levels of total AurB.
Cite Format: Affinity Biosciences Cat# AF6475, RRID:AB_2835294.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


AIK2; AIM-1; AIM1; ARK-2; ARK2; AurB; AURKB; AURKB_HUMAN; Aurora 1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora related kinase 2; Aurora- and Ipl1-like midbody-associated protein 1; Aurora-B; Aurora-related kinase 2; Aurora/IPL1 related kinase 2; Aurora/IPL1-related kinase 2; IPL1; PPP1R48; Protein phosphatase 1 regulatory subunit 48; Serine/theronine kinase 12; Serine/threonine protein kinase 12; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase aurora-B; STK-1; STK1; STK12; STK5;



High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96GD4 As Substrate

Site PTM Type Enzyme
K4 Ubiquitination
S7 Phosphorylation Q96GD4 (AURKB)
Y8 Phosphorylation
Y12 Phosphorylation
T16 Phosphorylation Q96GD4 (AURKB)
S19 Phosphorylation
K31 Ubiquitination
T35 Phosphorylation
S45 Phosphorylation
K56 Ubiquitination
S61 Phosphorylation
S62 Phosphorylation
T64 Phosphorylation
T73 Phosphorylation
K85 Sumoylation
K85 Ubiquitination
K87 Sumoylation
K87 Ubiquitination
Y92 Phosphorylation
K98 Ubiquitination
K110 Ubiquitination
K115 Ubiquitination
R147 Methylation
K164 Ubiquitination
K168 Ubiquitination
T179 Phosphorylation
K194 Ubiquitination
K202 Sumoylation
K202 Ubiquitination
K211 Ubiquitination
K215 Acetylation
K215 Ubiquitination
S227 Phosphorylation
K231 Ubiquitination
T232 Phosphorylation O14965 (AURKA) , Q96GD4 (AURKB) , Q13043 (STK4)
T236 Phosphorylation
Y239 Phosphorylation
K287 Ubiquitination
K291 Ubiquitination
K306 Ubiquitination
S313 Phosphorylation
S331 Phosphorylation O14757 (CHEK1) , PR:O14757 (hCHEK1)

PTMs - Q96GD4 As Enzyme

Substrate Site Source
O00566 (MPHOSPH10) T332 Uniprot
O00571 (DDX3X) S594 Uniprot
O14777 (NDC80) S5 Uniprot
O14777 (NDC80) S15 Uniprot
O14777 (NDC80) S44 Uniprot
O14777 (NDC80) T49 Uniprot
O14777 (NDC80) S55 Uniprot
O14777 (NDC80) S69 Uniprot
O14950 (MYL12B) S20 Uniprot
O15392 (BIRC5) T34 Uniprot
O15392 (BIRC5) T117 Uniprot
O60879 (DIAPH2) S196 Uniprot
O95235 (KIF20A) S878 Uniprot
O95239 (KIF4A) T799 Uniprot
P04637 (TP53) S183 Uniprot
P04637 (TP53) T211 Uniprot
P04637 (TP53) S215 Uniprot
P04637 (TP53) S269 Uniprot
P04637 (TP53) T284 Uniprot
P05204 (HMGN2) S25 Uniprot
P05204 (HMGN2) S29 Uniprot
P05787 (KRT8) T6 Uniprot
P06400 (RB1) S780 Uniprot
P08238 (HSP90AB1) S255 Uniprot
P08238 (HSP90AB1) S452 Uniprot
P08670 (VIM) S7 Uniprot
P08670 (VIM) S25 Uniprot
P08670 (VIM) S39 Uniprot
P08670 (VIM) S47 Uniprot
P08670 (VIM) S65 Uniprot
P08670 (VIM) S66 Uniprot
P08670 (VIM) S72 Uniprot
P08670 (VIM) S73 Uniprot
P08670 (VIM) S87 Uniprot
P10412 (HIST1H1E) S27 Uniprot
P14136 (GFAP) T7 Uniprot
P14136 (GFAP) S13 Uniprot
P14136 (GFAP) S38 Uniprot
P14618 (PKM) T45 Uniprot
P17661 (DES) S12 Uniprot
P17661 (DES) T17 Uniprot
P17661 (DES) S60 Uniprot
P18615 (NELFE) S131 Uniprot
P25490 (YY1) S180 Uniprot
P25490 (YY1) S184 Uniprot
P35659 (DEK) T67 Uniprot
P38159 (RBMX) S326 Uniprot
P45973 (CBX5) S92 Uniprot
P46013 (MKI67) S374 Uniprot
P46013 (MKI67) S538 Uniprot
P46778 (RPL21) S104 Uniprot
P46783 (RPS10) T118 Uniprot
P49450-1 (CENPA) S7 Uniprot
P53350 (PLK1) T210 Uniprot
P56524 (HDAC4) S265 Uniprot
P62917 (RPL8) S130 Uniprot
P68431 (HIST1H3J) S11 Uniprot
P68431 (HIST1H3J) S29 Uniprot
P78527 (PRKDC) S511 Uniprot
P82970 (HMGN5) S20 Uniprot
P82970 (HMGN5) S24 Uniprot
P84243 (H3F3B) S11 Uniprot
P98179 (RBM3) S147 Uniprot
Q02224 (CENPE) T422 Uniprot
Q02241-2 (KIF23) S708 Uniprot
Q02241 (KIF23) S911 Uniprot
Q08J23 (NSUN2) S139 Uniprot
Q09666 (AHNAK) T158 Uniprot
Q13315 (ATM) S1403 Uniprot
Q14980 (NUMA1) T1811 Uniprot
Q14980 (NUMA1) S1969 Uniprot
Q14980 (NUMA1) S2047 Uniprot
Q14980 (NUMA1) T2055 Uniprot
Q15149 (PLEC) T2886 Uniprot
Q17R91 (DIAPH2) T66 Uniprot
Q17R91 (DIAPH2) S196 Uniprot
Q17R91 (DIAPH2) S820 Uniprot
Q17R91 (DIAPH2) T882 Uniprot
Q3KR16 (PLEKHG6) T544 Uniprot
Q53HL2 (CDCA8) S154 Uniprot
Q53HL2 (CDCA8) S165 Uniprot
Q53HL2 (CDCA8) S219 Uniprot
Q53HL2 (CDCA8) S275 Uniprot
Q53HL2 (CDCA8) T278 Uniprot
Q53SF7 (COBLL1) S952 Uniprot
Q5UIP0 (RIF1) S2205 Uniprot
Q69YH5 (CDCA2) S893 Uniprot
Q6NZI2 (CAVIN1) S300 Uniprot
Q8IVT2 (MISP) S348 Uniprot
Q8IX03 (WWC1) S539 Uniprot
Q8IX90 (SKA3) S159 Uniprot
Q8NEM2 (SHCBP1) S634 Uniprot
Q8NEY8 (PPHLN1) S110 Uniprot
Q8NG31 (KNL1) S24 Uniprot
Q8NG31 (KNL1) S60 Uniprot
Q8TE77 (SSH3) S37 Uniprot
Q8TEW0 (PARD3) S962 Uniprot
Q8TF76 (HASPIN) S93 Uniprot
Q8TF76 (HASPIN) S143 Uniprot
Q8WWI1 (LMO7) S300 Uniprot
Q8WWI1 (LMO7) S751 Uniprot
Q8WWK9 (CKAP2) S628 Uniprot
Q8WYJ6 (SEPT1) S248 Uniprot
Q8WYJ6 (SEPT1) S307 Uniprot
Q8WYJ6 (SEPT1) S315 Uniprot
Q96CF2 (CHMP4C) S210 Uniprot
Q96CF2 (CHMP4C) S214 Uniprot
Q96CF2 (CHMP4C) S215 Uniprot
Q96FF9 (CDCA5) S33 Uniprot
Q96FF9 (CDCA5) S79 Uniprot
Q96FF9 (CDCA5) S83 Uniprot
Q96FF9 (CDCA5) T111 Uniprot
Q96FF9 (CDCA5) T115 Uniprot
Q96FF9 (CDCA5) S126 Uniprot
Q96FF9 (CDCA5) S139 Uniprot
Q96FF9 (CDCA5) T151 Uniprot
Q96FF9 (CDCA5) T159 Uniprot
Q96FF9 (CDCA5) S164 Uniprot
Q96GD4 (AURKB) S7 Uniprot
Q96GD4 (AURKB) T16 Uniprot
Q96GD4 (AURKB) T232 Uniprot
Q96PK6 (RBM14) S649 Uniprot
Q99661 (KIF2C) S95 Uniprot
Q99661 (KIF2C) S109 Uniprot
Q99661 (KIF2C) S111 Uniprot
Q99661 (KIF2C) S115 Uniprot
Q99661 (KIF2C) S192 Uniprot
Q9BQG0 (MYBBP1A) S1303 Uniprot
Q9BW61 (DDA1) S33 Uniprot
Q9BXS6 (NUSAP1) S240 Uniprot
Q9H0H5 (RACGAP1) S144 Uniprot
Q9H0H5 (RACGAP1) T145 Uniprot
Q9H0H5 (RACGAP1) S185 Uniprot
Q9H0H5 (RACGAP1) T186 Uniprot
Q9H0H5 (RACGAP1) S187 Uniprot
Q9H0H5 (RACGAP1) T249 Uniprot
Q9H0H5 (RACGAP1) S387 Uniprot
Q9H0H5 (RACGAP1) S410 Uniprot
Q9H410 (DSN1) S100 Uniprot
Q9H410 (DSN1) S109 Uniprot
Q9H6F5 (CCDC86) S255 Uniprot
Q9H788 (SH2D4A) S261 Uniprot
Q9NP77 (SSU72) S19 Uniprot
Q9NQS7 (INCENP) T59 Uniprot
Q9NQS7 (INCENP) S72 Uniprot
Q9NQS7 (INCENP) T494 Uniprot
Q9NQS7 (INCENP) S518 Uniprot
Q9NQS7-2 (INCENP) T888 Uniprot
Q9NQS7-2 (INCENP) S889 Uniprot
Q9NQS7-2 (INCENP) S890 Uniprot
Q9NQS7 (INCENP) T892 Uniprot
Q9NQS7 (INCENP) S893 Uniprot
Q9NQS7 (INCENP) S894 Uniprot
Q9NQS7 (INCENP) S899 Uniprot
Q9NQZ2 (UTP3) S462 Uniprot
Q9NS23-2 (RASSF1) S203 Uniprot
Q9NS23 (RASSF1) S207 Uniprot
Q9UBB4 (ATXN10) S12 Uniprot
Q9UJ70 (NAGK) S76 Uniprot
Q9UKV0 (HDAC9) S239 Uniprot
Q9UPY8 (MAPRE3) S176 Uniprot
Q9UQL6 (HDAC5) S278 Uniprot
Q9Y2I6 (NINL) S185 Uniprot
Q9Y2I6 (NINL) S448 Uniprot
Q9Y2I6 (NINL) S585 Uniprot
Q9Y2R4 (DDX52) S22 Uniprot
Q9Y5B9 (SUPT16H) T494 Uniprot

Research Backgrounds


Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPTIN1, VIM/vimentin, HASPIN, and histone H3. A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between HASPIN and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGO1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes.


The phosphorylation of Thr-232 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-232 phosphorylation is indispensable for the AURKB kinase activity.

Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.

Subcellular Location:

Nucleus. Chromosome. Chromosome>Centromere. Cytoplasm>Cytoskeleton>Spindle. Midbody.
Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the midbody. Proper localization of the active, Thr-232-phosphorylated form during metaphase may be dependent upon interaction with SPDYC. Colocalized with SIRT2 during cytokinesis with the midbody. Localization (and probably targeting of the CPC) to the inner centromere occurs predominantly in regions with overlapping mitosis-specific histone phosphorylations H3pT3 and H2ApT12.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

Subunit Structure:

Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; predominantly independent AURKB- and AURKC-containing complexes exist. Associates with RACGAP1 during M phase. Interacts with CDCA1, EVI5, JTB, NDC80, PSMA3, SEPTIN1, SIRT2 and TACC1. Interacts with SPDYC; this interaction may be required for proper localization of active, Thr-232-phosphorylated AURKB form during prometaphase and metaphase. Interacts with p53/TP53. Interacts (via the middle kinase domain) with NOC2L (via the N- and C-terminus domains). Interacts with TTC28. Interacts with RNF2/RING1B.


Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

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