Nrf2 Antibody - #AF7006
Product: | Nrf2 Antibody |
Catalog: | AF7006 |
Description: | Rabbit polyclonal antibody to Nrf2 |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
Mol.Wt.: | 68kDa,125kDa; 68kD(Calculated). |
Uniprot: | Q16236 |
RRID: | AB_2835314 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF7006, RRID:AB_2835314.
Fold/Unfold
erythroid derived 2; HEBP1; like 2; NF E2 related factor 2; NF-E2-related factor 2; NF2L2_HUMAN; NFE2 related factor 2; NFE2-related factor 2; Nfe2l2; Nrf 2; NRF2; Nuclear factor (erythroid derived 2) like 2; Nuclear factor; nuclear factor erythroid 2 like 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid 2-related factor 2; Nuclear factor erythroid derived 2 like 2;
Immunogens
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
- Q16236 NF2L2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMDLELPPPGLPSQQDMDLIDILWRQDIDLGVSREVFDFSQRRKEYELEKQKKLEKERQEQLQKEQEKAFFAQLQLDEETGEFLPIQPAQHIQSETSGSANYSQVAHIPKSDALYFDDCMQLLAQTFPFVDDNEVSSATFQSLVPDIPGHIESPVFIATNQAQSPETSVAQVAPVDLDGMQQDIEQVWEELLSIPELQCLNIENDKLVETTMVPSPEAKLTEVDNYHFYSSIPSMEKEVGNCSPHFLNAFEDSFSSILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMPSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDAQCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLLKEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDVKKN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q16236 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S40 | Phosphorylation | P05771 (PRKCB) , P17252 (PRKCA) | Uniprot |
K44 | Ubiquitination | Uniprot | |
K64 | Ubiquitination | Uniprot | |
T80 | Phosphorylation | Uniprot | |
S215 | Phosphorylation | Uniprot | |
S301 | Phosphorylation | Uniprot | |
S344 | Phosphorylation | P49841 (GSK3B) | Uniprot |
S347 | Phosphorylation | P49841 (GSK3B) | Uniprot |
S351 | Phosphorylation | Uniprot | |
S356 | Phosphorylation | Uniprot | |
T395 | Phosphorylation | Q00535 (CDK5) | Uniprot |
S408 | Phosphorylation | Uniprot | |
S433 | Phosphorylation | Q00535 (CDK5) | Uniprot |
R437 | Methylation | Uniprot | |
K438 | Acetylation | Uniprot | |
T439 | Phosphorylation | Q00535 (CDK5) | Uniprot |
K443 | Acetylation | Uniprot | |
K445 | Acetylation | Uniprot | |
S447 | Phosphorylation | Uniprot | |
K462 | Acetylation | Uniprot | |
K462 | Ubiquitination | Uniprot | |
K472 | Acetylation | Uniprot | |
K487 | Acetylation | Uniprot | |
K506 | Acetylation | Uniprot | |
K508 | Acetylation | Uniprot | |
K516 | Acetylation | Uniprot | |
K516 | Ubiquitination | Uniprot | |
K518 | Acetylation | Uniprot | |
K518 | Sumoylation | Uniprot | |
K518 | Ubiquitination | Uniprot | |
K533 | Acetylation | Uniprot | |
K536 | Acetylation | Uniprot | |
K538 | Acetylation | Uniprot | |
K541 | Acetylation | Uniprot | |
K543 | Acetylation | Uniprot | |
K548 | Acetylation | Uniprot | |
K548 | Ubiquitination | Uniprot | |
K554 | Acetylation | Uniprot | |
K554 | Ubiquitination | Uniprot | |
K555 | Acetylation | Uniprot | |
S558 | Phosphorylation | Uniprot | |
T559 | Phosphorylation | Uniprot | |
Y561 | Phosphorylation | Uniprot | |
K574 | Ubiquitination | Uniprot | |
Y576 | Phosphorylation | P06241 (FYN) | Uniprot |
S577 | Phosphorylation | Uniprot | |
K596 | Acetylation | Uniprot | |
K599 | Acetylation | Uniprot |
Research Backgrounds
Transcription factor that plays a key role in the response to oxidative stress: binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles. In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex. In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes. The NFE2L2/NRF2 pathway is also activated in response to selective autophagy: autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes. May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
Ubiquitinated in the cytoplasm by the BCR(KEAP1) E3 ubiquitin ligase complex leading to its degradation. In response to oxidative stress, electrophile metabolites, such as sulforaphane, modify KEAP1, leading to inhibit activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and activity. In response to autophagy, the BCR(KEAP1) complex is inactivated (By similarity).
Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
Acetylation at Lys-596 and Lys-599 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence.
Glycation impairs transcription factor activity by preventing heterodimerization with small Maf proteins. Deglycation by FN3K restores activity.
Cytoplasm>Cytosol. Nucleus.
Note: Cytosolic under unstressed conditions: ubiquitinated and degraded by the BCR(KEAP1) E3 ubiquitin ligase complex (PubMed:15601839, PubMed:21196497). Translocates into the nucleus upon induction by electrophilic agents that inactivate the BCR(KEAP1) E3 ubiquitin ligase complex (PubMed:21196497).
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Heterodimer; heterodimerizes with small Maf proteins (By similarity). Interacts (via the bZIP domain) with MAFG and MAFK; required for binding to antioxidant response elements (AREs) on DNA (By similarity). Interacts with KEAP1; the interaction is direct and promotes ubiquitination by the BCR(KEAP1) E3 ubiquitin ligase complex. Forms a ternary complex with PGAM5 and KEAP1. Interacts with EEF1D at heat shock promoter elements (HSE). Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1. Interacts with CHD6; involved in activation of the transcription (By similarity). Interacts with ESRRB; represses NFE2L2 transcriptional activity (By similarity).
(Microbial infection) Interacts with herpes virus 8 protein LANA1.
The ETGE motif, and to a lower extent the DLG motif, mediate interaction with KEAP1.
Belongs to the bZIP family. CNC subfamily.
Research Fields
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
References
Application: IHC Species: mice Sample: tubular epithelial cells
Application: WB Species: mice Sample: BMDMs
Application: WB Species: Rat Sample: PC-12 cells
Application: WB Species: Human Sample: kidney tissue
Application: IHC Species: Mouse Sample:
Application: WB Species: mouse Sample: liver
Application: IHC Species: mouse Sample: liver
Application: WB Species: mouse Sample: RAW264.7 macrophages
Application: WB Species: Rat Sample: heart tissues
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