AFfirm™ ELAVL1 Mouse Monoclonal Antibody - #BF8063

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Product: ELAVL1 Mouse Monoclonal Antibody
Catalog: BF8063
Description: Mouse monoclonal antibody to ELAVL1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 36kDa; 36kD(Calculated).
Uniprot: Q15717

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:1000-1:10000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8063]
Specificity:
ELAVL1 Antibody detects endogenous levels of total ELAVL1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ELAV (embryonic lethal abnormal vision Drosophila) like 1; ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R); ELAV like 1; ELAV like RNA binding protein 1; ELAV-like protein 1; ELAV1; ELAV1_HUMAN; Elavl1; Embryonic lethal abnormal vision drosophila homolog like 1; Hu Antigen R; Hu-antigen R; Hua; HuR; MelG;

Immunogens

Immunogen:
Uniprot:

Q15717(ELAV1_HUMAN) >>Visit HPA database.

Gene(ID):
ELAVL1(1994)
Expression:
Q15717 ELAV1_HUMAN:

Ubiquitous. Detected in brain, liver, thymus and muscle.

Description:
The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012]
Sequence:
MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK

PTMs - Q15717 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
Y5 Phosphorylation
R14 Methylation
Y26 Phosphorylation
T32 Phosphorylation
S38 Phosphorylation
S41 Phosphorylation
S48 Phosphorylation
K50 Ubiquitination
K55 Ubiquitination
Y63 Phosphorylation P52333 (JAK3)
Y68 Phosphorylation P52333 (JAK3)
K72 Ubiquitination
T80 Phosphorylation
S88 Phosphorylation O96017 (CHEK2)
K89 Ubiquitination
K92 Ubiquitination
S100 Phosphorylation O96017 (CHEK2)
K104 Ubiquitination
Y109 Phosphorylation
S111 Phosphorylation
T118 Phosphorylation Q16539 (MAPK14) , O96017 (CHEK2)
K120 Ubiquitination
T143 Phosphorylation
S146 Phosphorylation
R147 Methylation
S158 Phosphorylation P17252 (PRKCA)
K171 Ubiquitination
K182 Ubiquitination
K191 Ubiquitination
S197 Phosphorylation
Y200 Phosphorylation P52333 (JAK3)
S202 Phosphorylation P06493 (CDK1) , Q00535 (CDK5) , P24941 (CDK2)
R205 Methylation
R206 Methylation
R217 Methylation
R219 Methylation
S221 Phosphorylation Q05655 (PRKCD) , P17252 (PRKCA)
S242 Phosphorylation
K283 Ubiquitination
S304 Phosphorylation O15111 (CHUK)
K313 Ubiquitination
S318 Phosphorylation Q05655 (PRKCD) , P17252 (PRKCA)
K320 Acetylation
K320 Methylation
K320 Ubiquitination
K323 Ubiquitination
K326 Ubiquitination

Research Backgrounds

Function:

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR.

PTMs:

Phosphorylated by MAPKAPK2. Phosphorylated by PRKCD.

Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Stress granule.
Note: Translocates into the cytoplasm following phosphorylation by MAPKAPK2 (PubMed:14517288). Likewise, phosphorylation by PRKCD promotes translocation from the nucleus into the cytoplasm, where it is associated with free and cytoskeleton-bound polysomes (PubMed:18285462).Localizes to the stress granules in the presence of PLEKHN1 (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Detected in brain, liver, thymus and muscle.

Subunit Structure:

Monomer and homodimer (in vitro). Interacts with ANP32A. Interacts with ZNF385A; the interaction is indirect and mRNA-dependent and may regulate p53/TP53 expression (By similarity). Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with AGO1 and AGO2. Interacts with IGF2BP2 and IGF2BP3. Interacts with HNRNPL. Interacts with DHX36; this interaction occurs in a RNA-dependent manner. Interacts with ILF3; this interaction occurs in a RNA-dependent manner. Interacts with PLEKHN1. Interacts with SHFL; the interaction increases in presence of RNA. Interacts with YBX1; interaction recruits ELAVL1 on C5-methylcytosine (m5C)-containing mRNAs, thereby promoting mRNA stability.

Family&Domains:

The first RRM (RNA recognition motif) domain is essential for binding to AU-rich elements.

Belongs to the RRM elav family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

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