Product: NKX6.3 Antibody
Catalog: AF0302
Description: Rabbit polyclonal antibody to NKX6.3
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 34kDa; 29kD(Calculated).
Uniprot: A6NJ46
RRID: AB_2833466

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC: 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
NKX6.3 Antibody detects endogenous levels of total NKX6.3.
RRID:
AB_2833466
Cite Format: Affinity Biosciences Cat# AF0302, RRID:AB_2833466.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ25169; Homeobox protein Nkx 6.3; Homeobox protein Nkx-6.3; NK6 homeobox 3; NKX6-3; NKX6.3; NKX63; NKX63_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Nkx6-3 Putative transcription factor, which may be involved in patterning of central nervous system and pancreas.
Sequence:
MESNLQGTFLLNNTPLAQFPEMKAPVCQYSVQNSFYKLSPPGLGPQLAAGTPHGITDILSRPVAAPNNSLLSGYPHVAGFGGLSSQGVYYSPQVGNFSKAGNEYPTRTRNCWADTGQDWRGGRQCSNTPDPLSDSIHKKKHTRPTFTGHQIFALEKTFEQTKYLAGPERARLAYSLGMTESQVKVWFQNRRTKWRKKSALEPSSSTPRAPGGAGAGAGGDRAPSENEDDEYNKPLDPDSDDEKIRLLLRKHRAAFSVLSLGAHSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
91
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - A6NJ46 As Substrate

Site PTM Type Enzyme
K156 Acetylation
K162 Acetylation
S198 Phosphorylation
S205 Phosphorylation
T206 Phosphorylation

Research Backgrounds

Function:

Putative transcription factor, which may be involved in patterning of central nervous system and pancreas.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Restrictive clause

 

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For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.