AFfirm™ PGP9.5 Mouse Monoclonal Antibody - #BF8240

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Product: PGP9.5 Mouse Monoclonal Antibody
Catalog: BF8240
Description: Mouse monoclonal antibody to PGP9.5
Application: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 25 kDa; 25kD(Calculated).
Uniprot: P09936

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   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8240]
Specificity:
PGP9.5 Mouse Monoclonal Antibody detects endogenous levels of total PGP9.5
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Epididymis luminal protein 117; Epididymis secretory protein Li 53; HEL 117; HEL S 53; NDGOA; Neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; Park 5; PARK5; PGP 9.5; PGP9.5; PGP95; Protein gene product 9.5; Ubiquitin C terminal esterase L1; Ubiquitin C terminal hydrolase; Ubiquitin C terminal hydrolase L1; Ubiquitin carboxyl terminal esterase L1; Ubiquitin carboxyl terminal hydrolase isozyme L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1; Ubiquitin thiolesterase; Ubiquitin thiolesterase L1; UCH-L1; UCHL1; UCHL1_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human PGP9.5, corresponding to a region within C-terminal amino acids.

Uniprot:

P09936(UCHL1_HUMAN) >>Visit HPA database.

Gene(ID):
UCHL1(7345)
Expression:
P09936 UCHL1_HUMAN:

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

Description:
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes.
Sequence:
MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA

Research Backgrounds

Function:

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity.

PTMs:

O-glycosylated.

Subcellular Location:

Cytoplasm. Endoplasmic reticulum membrane>Lipid-anchor.
Note: About 30% of total UCHL1 is associated with membranes in brain.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

Family&Domains:

Belongs to the peptidase C12 family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

References

1). Effects of icariin as a feed additive on the reproductive function in bucks (Capra hircus). Frontiers in veterinary science, 2024 (PubMed: 39575435) [IF=3.2]

Application: WB    Species: goat    Sample:

Figure 5. Effect of ICA administration on male germ cell markers of dairy goats. (A) Confocal microscopy analyzes the distribution of PGP9.5, DDX4, and c-Kit in the testes. (a) Immunofluorescence analysis showed the expression of PGP9.5 and DDX4. (b) The fluorescence intensity was analyzed on the yellow lines by Fiji software. (c) Immunofluorescence analysis showed the distribution of PGP9.5 and c-Kit. (d) The fluorescence intensity was analyzed on the yellow lines by Fiji software. (B) qPCR and western blot analyze the expression of mRNA and proteins. (a) The mRNA levels of PGP9.5, DDX4, and c-Kit were examined by qPCR. (b) Western blot analysis was performed to measure the expression of target proteins. β-actin was utilized as a loading control. (c) The histogram represents the quantification of proteins levels. Each value represents the mean ± SEM, **p 

Application: IF/ICC    Species: goat    Sample:

Figure 5. Effect of ICA administration on male germ cell markers of dairy goats. (A) Confocal microscopy analyzes the distribution of PGP9.5, DDX4, and c-Kit in the testes. (a) Immunofluorescence analysis showed the expression of PGP9.5 and DDX4. (b) The fluorescence intensity was analyzed on the yellow lines by Fiji software. (c) Immunofluorescence analysis showed the distribution of PGP9.5 and c-Kit. (d) The fluorescence intensity was analyzed on the yellow lines by Fiji software. (B) qPCR and western blot analyze the expression of mRNA and proteins. (a) The mRNA levels of PGP9.5, DDX4, and c-Kit were examined by qPCR. (b) Western blot analysis was performed to measure the expression of target proteins. β-actin was utilized as a loading control. (c) The histogram represents the quantification of proteins levels. Each value represents the mean ± SEM, **p 

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