Niban Antibody - #AF4008

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Product: Niban Antibody
Catalog: AF4008
Description: Rabbit polyclonal antibody to Niban
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 150kd; 103kD(Calculated).
Uniprot: Q9BZQ8
RRID: AB_2835335

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Niban Antibody detects endogenous levels of total Niban.
RRID:
AB_2835335
Cite Format: Affinity Biosciences Cat# AF4008, RRID:AB_2835335.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cell growth inhibiting gene 39 protein; Cell growth-inhibiting gene 39 protein; FAM129A; NIBAN_HUMAN; Protein FAM129A; Protein Niban;

Immunogens

Immunogen:
Uniprot:

Q9BZQ8(NIBA1_HUMAN) >>Visit HPA database.

Gene(ID):
FAM129A(116496)
Expression:
Q9BZQ8 NIBA1_HUMAN:

Expressed in various types of thyroid tumor such as papillary thyroid carcinomas and oxyphilic thyroid tumors but not in normal thyroid tissue (at protein level). Strongly expressed in heart, skeletal muscle, pancreas, white blood cells and prostate with moderate expression in colon and spleen. Expressed in renal carcinoma cells but not in normal kidney.

Description:
Regulates phosphorylation of a number of proteins involved in translation regulation including EIF2A, EIF4EBP1 and RPS6KB1. May be involved in the endoplasmic reticulum stress response
Sequence:
MGGSASSQLDEGKCAYIRGKTEAAIKNFSPYYSRQYSVAFCNHVRTEVEQQRDLTSQFLKTKPPLAPGTILYEAELSQFSEDIKKWKERYVVVKNDYAVESYENKEAYQRGAAPKCRILPAGGKVLTSEDEYNLLSDRHFPDPLASSEKENTQPFVVLPKEFPVYLWQPFFRHGYFCFHEAADQKRFSALLSDCVRHLNHDYMKQMTFEAQAFLEAVQFFRQEKGHYGSWEMITGDEIQILSNLVMEELLPTLQTDLLPKMKGKKNDRKRTWLGLLEEAYTLVQHQVSEGLSALKEECRALTKGLEGTIRSDMDQIVNSKNYLIGKIKAMVAQPAEKSCLESVQPFLASILEELMGPVSSGFSEVRVLFEKEVNEVSQNFQTTKDSVQLKEHLDRLMNLPLHSVKMEPCYTKVNLLHERLQDLKSRFRFPHIDLVVQRTQNYMQELMENAVFTFEQLLSPHLQGEASKTAVAIEKVKLRVLKQYDYDSSTIRKKIFQEALVQITLPTVQKALASTCKPELQKYEQFIFADHTNMIHVENVYEEILHQILLDETLKVIKEAAILKKHNLFEDNMALPSESVSSLTDLKPPTGSNQASPARRASAILPGVLGSETLSNEVFQESEEEKQPEVPSSLAKGESLSLPGPSPPPDGTEQVIISRVDDPVVNPVATEDTAGLPGTCSSELEFGGTLEDEEPAQEEPEPITASGSLKALRKLLTASVEVPVDSAPVMEEDTNGESHVPQENEEEEEKEPSQAAAIHPDNCEESEVSEREAQPPCPEAHGEELGGFPEVGSPASPPASGGLTEEPLGPMEGELPGEACTLTAHEGRGGKCTEEGDASQQEGCTLGSDPICLSESQVSEEQEEMGGQSSAAQATASVNAEEIKVARIHECQWVVEDAPNPDVLLSHKDDVKEGEGGQESFPELPSEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
92
Chicken
75
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BZQ8 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
S4 Phosphorylation
K26 Acetylation
S37 Phosphorylation
K60 Ubiquitination
K62 Ubiquitination
K84 Ubiquitination
K94 Ubiquitination
K105 Ubiquitination
K124 Ubiquitination
Y132 Phosphorylation
R138 Methylation
K149 Ubiquitination
T152 Phosphorylation
K185 Acetylation
T271 Phosphorylation
T281 Phosphorylation
S288 Phosphorylation
S292 Phosphorylation
K303 Ubiquitination
K320 Ubiquitination
K326 Ubiquitination
K328 Ubiquitination
S349 Phosphorylation
K371 Ubiquitination
K384 Ubiquitination
K390 Ubiquitination
S403 Phosphorylation
K405 Ubiquitination
K412 Ubiquitination
K475 Ubiquitination
K482 Ubiquitination
T490 Phosphorylation
K517 Ubiquitination
K558 Ubiquitination
K565 Acetylation
K565 Ubiquitination
S577 Phosphorylation
S579 Phosphorylation
S581 Phosphorylation
S582 Phosphorylation
T584 Phosphorylation
T590 Phosphorylation
S592 Phosphorylation
S596 Phosphorylation
S602 Phosphorylation P31749 (AKT1)
S611 Phosphorylation
S622 Phosphorylation
K626 Ubiquitination
S639 Phosphorylation
S641 Phosphorylation
S646 Phosphorylation
T704 Phosphorylation
S708 Phosphorylation
T717 Phosphorylation
S719 Phosphorylation
K908 Ubiquitination
S920 Phosphorylation
S926 Phosphorylation

Research Backgrounds

Function:

Regulates phosphorylation of a number of proteins involved in translation regulation including EIF2A, EIF4EBP1 and RPS6KB1. May be involved in the endoplasmic reticulum stress response (By similarity).

Subcellular Location:

Cytoplasm. Membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in various types of thyroid tumor such as papillary thyroid carcinomas and oxyphilic thyroid tumors but not in normal thyroid tissue (at protein level). Strongly expressed in heart, skeletal muscle, pancreas, white blood cells and prostate with moderate expression in colon and spleen. Expressed in renal carcinoma cells but not in normal kidney.

Family&Domains:

Belongs to the Niban family.

References

1). Chinese Herbal Medicine Suyin Detoxification Granule Inhibits Pyroptosis and Epithelial-Mesenchymal Transition by Downregulating MAVS/NLRP3 to Alleviate Renal Injury. Journal of Inflammation Research, 2021 (PubMed: 34908861) [IF=4.5]

Application: WB    Species: Rat    Sample:

Figure 3 Albumin induced renal tubular epithelial cell injury in vitro. (A) The effect of BSA on cell viability. HK-2 cells in 96-well plates were exposed to the different concentrations (0, 5, 10, and 20 g/L) of BSA for 12, 24, and 48 h. Cell viability was evaluated using CCK-8 assay. (B, C) The effect of BSA on the cell migration capacity and associated morphological changes. Cell morphology and scratch assays were performed using phase-contrast microscopy (magnification, 100X). (D) The effect of BSA on Vimentin, E-cadherin (E-cad), Fibronectin (FN) and Collagen type I (Col I) protein expression. After incubation for 24 h, cell lysates were subjected to Western blotting to measure Vimentin, E-cadherin, Fibronectin and Collagen type I levels. (E) The effect of BSA on NLRP3 inflammasome proteins. After incubation for 24 h, cell lysates were subjected to Western blotting to measure NLRP3, ASC, Pro Caspase-1 with cleaved caspase −1 levels, N-terminal of Gasdermin D (GSDMD-N), and Pro IL-1β with cleaved IL-1β. Data are expressed as means ± SD; n = 3. #P < 0.05; ##P < 0.01 vs control.

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