N Cadherin Antibody | Affinity Biosciences

Product:	N Cadherin Antibody
Catalog:	AF4039
Description:	Rabbit polyclonal antibody to N Cadherin
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	140kd; 100kD(Calculated).
Uniprot:	P19022
RRID:	AB_2835344

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

N Cadherin Antibody detects endogenous levels of total N Cadherin.

RRID:

AB_2835344
Cite Format: Affinity Biosciences Cat# AF4039, RRID:AB_2835344.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CADH2_HUMAN; Cadherin 2; Cadherin 2 N cadherin neuronal; Cadherin 2 type 1; Cadherin 2 type 1 N cadherin neuronal; Cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; Calcium dependent adhesion protein neuronal; CD325; CD325 antigen; CDH2; CDHN; CDw325; CDw325 antigen; N cadherin 1; N-cadherin; NCAD; Neural cadherin; OTTHUMP00000066304; OTTHUMP00000067378;

Immunogens

Immunogen:

Uniprot:

P19022(CADH2_HUMAN) >>Visit HPA database.

Gene(ID):

CDH2(1000)

Description:

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density

Sequence:

P19022 CADH2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MCRIAGALRTLLPLLAALLQASVEASGEIALCKTGFPEDVYSAVLSKDVHEGQPLLNVKFSNCNGKRKVQYESSEPADFKVDEDGMVYAVRSFPLSSEHAKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQATDMEGNPTYGLSNTATAVITVTDVNDNPPEFTAMTFYGEVPENRVDIIVANLTVTDKDQPHTPAWNAVYRISGGDPTGRFAIQTDPNSNDGLVTVVKPIDFETNRMFVLTVAAENQVPLAKGIQHPPQSTATVSVTVIDVNENPYFAPNPKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLSDPANWLKIDPVNGQITTIAVLDRESPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLDINDNAPQVLPQEAETCETPDPNSINITALDYDIDPNAGPFAFDLPLSPVTIKRNWTITRLNGDFAQLNLKIKFLEAGIYEVPIIITDSGNPPKSNISILRVKVCQCDSNGDCTDVDRIVGAGLGTGAIIAILLCIIILLILVLMFVVWMKRRDKERQAKQLLIDPEDDVRDNILKYDEEGGGEEDQDYDLSQLQQPDTVEPDAIKPVGIRRMDERPIHAEPQYPVRSAAPHPGDIGDFINEGLKAADNDPTAPPYDSLLVFDYEGSGSTAGSLSSLNSSSSGGEQDYDYLNDWGPRFKKLADMYGGGDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19022 As Substrate

P19022

Site	PTM Type	Enzyme	Source
S61	Phosphorylation		Uniprot
S96	Phosphorylation		Uniprot
S135	Phosphorylation		Uniprot
N273	N-Glycosylation		Uniprot
N402	N-Glycosylation		Uniprot
K448	Ubiquitination		Uniprot
T454	Phosphorylation		Uniprot
Y496	Phosphorylation		Uniprot
K536	Ubiquitination		Uniprot
N572	N-Glycosylation		Uniprot
K667	Ubiquitination		Uniprot
T683	Phosphorylation		Uniprot
S685	Phosphorylation		Uniprot
N692	N-Glycosylation		Uniprot
K756	Ubiquitination		Uniprot
K772	Ubiquitination		Uniprot
Y773	Phosphorylation		Uniprot
Y785	Phosphorylation		Uniprot
S788	Phosphorylation		Uniprot
K802	Ubiquitination		Uniprot
Y820	Phosphorylation	P12931 (SRC)	Uniprot
S824	Phosphorylation		Uniprot
Y852	Phosphorylation	P12931 (SRC)	Uniprot
Y860	Phosphorylation	P12931 (SRC)	Uniprot
Y884	Phosphorylation	P12931 (SRC)	Uniprot
Y886	Phosphorylation	P12931 (SRC)	Uniprot

Research Backgrounds

P19022_CADH2_HUMAN

Function:

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

PTMs:

Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).

May be phosphorylated by OBSCN.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell membrane>Sarcolemma. Cell junction. Cell surface.
Note: Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes.

Subunit Structure:

Homodimer (via extracellular region). Can also form heterodimers with other cadherins (via extracellular region). Dimerization occurs in trans, i.e. with a cadherin chain from another cell (By similarity). Interacts with CDCP1. Interacts with PCDH8; this complex may also include TAOK2 (By similarity). The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway (By similarity). Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. May interact with OBSCN (via protein kinase domain 2) (By similarity).

Family&Domains:

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. Calcium-binding sites are occupied sequentially in the order of site 3, then site 2 and site 1.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

References

1). METTL13 Mediates the Translation of Snail in Head and Neck Squamous Cell Carcinoma. International Journal of Oral Science, 2020 (PubMed: 34381012) [IF=10.8]

2). Huaier polysaccharides suppress triple-negative breast cancer metastasis and epithelial-mesenchymal transition by inducing autophagic degradation of Snail. Cell and Bioscience, 2021 (PubMed: 34481526) [IF=7.5]

Application: WB Species: human Sample: breast cancer cells

Fig. 1| PS-T inhibits invasion, migration and EMT in breast cancer cells in vitro and in vivo. d 4 T-1 and MDA-MB-231 cells are treated with 5 μg/mL PS-T for 24 h. The levels of each EMT marker are quantifed using the NIH ImageJ software. (mean ± SD, *P < 0.05 and **P < 0.01)

Application: WB Species: Human Sample: breast cancer cells

Fig. 1 PS-T inhibits invasion, migration and EMT in breast cancer cells in vitro and in vivo. a The invasiveness of 4 T-1 and MDA-MB-231 cells after treatment with PS-T at 5 μg/mL for 12 h is evaluated using Transwell invasion assays at 24 h (mean ± SD, ***P < 0.001). b Migratory ability of 4 T-1 and MDA-MB-231 cells after treatment with PS-T at different concentrations is evaluated using scratch assays at different time points (mean ± SD, **P < 0.01, ***P < 0.001). c Mice are treated with normal saline (control), 25 μg/g or 100 μg/g PS-T by oral gavage every other day for 21 days (mean ± SD, *P < 0.05, **P < 0.01, scale bars: 100 μm). d 4 T-1 and MDA-MB-231 cells are treated with 5 μg/mL PS-T for 24 h. The levels of each EMT marker are quantified using the NIH ImageJ software. (mean ± SD, *P < 0.05 and **P < 0.01)

3). TRIP13 interference inhibits the proliferation and metastasis of thyroid cancer cells through regulating TTC5/p53 pathway and epithelial-mesenchymal transition related genes expression. BIOMEDICINE & PHARMACOTHERAPY, 2019 (PubMed: 31648166) [IF=6.9]

4). d-Borneol enhances cisplatin sensitivity via autophagy dependent EMT signaling and NCOA4-mediated ferritinophagy. PHYTOMEDICINE, 2022 (PubMed: 36030746) [IF=6.7]

5). Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma. LIVER INTERNATIONAL, 2022 (PubMed: 34854209) [IF=6.0]

Application: WB Species: Human Sample: HepG2 and Huh7 cells

FIGURE 7 A, Representative images of the western blot results for uPAR, MMP2 and MMP9 in ALB knockdown HepG2 and Huh7 cells; B, Zymography analysis illustrates MMP2 and MMP9 activity in ALB knockdown HepG2 and Huh7 cells; C, Quantitative analysis results and representative images of the western blot results for the EMT‐associated markers, E‐cadherin, N‐cadherin, vimentin, Snail and Twist by western blot in ALB knockdown HepG2 and Huh7 cells; D, Quantification shows a significantly higher uPAR in HCC group with ALB <3.5 g/dL compared to ALB ≥3.5 g/dL (*P < .05); E, Scatterplot showing the correlation between plasma levels of ALB and uPAR. The vertical position represents the expression levels of uPAR (lg pg/mL)

6). Polydopamine/carboxylic graphene oxide-composited polypyrrole films for promoting adhesion and alignment of Schwann cells. Colloids and surfaces-B, Biointerfaces, 2020 (PubMed: 32203860) [IF=5.4]

Application: WB Species: Sample: RSCs

Fig. 3.| Cell images of immunofluorescence staining for (a) N-cadherin expression, phalloidin, (b) GFAP, (c) S100 and DAPI, and scale bar is 50 μm. (d) WB assay for Vinculin, Ncadherin, P75 and GFAP proteins and (e) their semi-quantitative comparison. & shows p＜0.05, compared with control; # shows p＜0.05, compared with PPy-PLLA; Δ shows p＜0.05, compared with CGO/PPy-PLLA.

Application: IF/ICC Species: Sample: RSCs

7). Tspan5 promotes epithelial–mesenchymal transition and tumour metastasis of hepatocellular carcinoma by activating Notch signalling. Molecular Oncology, 2021 (PubMed: 33955149) [IF=5.0]

Application: WB Species: Human Sample: HCC cells

Fig. 3 Tspan5 promotes EMT of HCC cells. (A) Tspan5 triggers the rearrangement of actin cytoskeleton. Stress fibres and actin filaments were visualized by Rhodamine-phalloidin staining (red) in HCC cells transduced with lentivirus containing Tspan5/shTspan5-encoding vector or empty/scramble RNA vector (Control/shControl). Nuclei were stained with DAPI (blue). Upregulation of Tspan5 significantly increased F-actin expression (red) and actin stress fibres throughout the Tspan5-upregulating cells compared with the control cells in which the actin bundles were predominantly localized underneath cell membranes. 400× magnification, scale bar: 10 μm. Mean ± SD, n = 3, Student's t-test; *P < 0.05, **P < 0.01. (B) Western blotting showing regulation of the expression of EMT markers, E-cadherin, N-cadherin, vimentin and Snail by Tspan5. GAPDH was used as a protein loading control. Numbers indicating relative protein ratio measured by Image J software and normalized to GAPDH. Vimentin was undetectable in MHCC97L cells. (C) Representative IF images showing regulation of the expressions of E-cadherin and vimentin by Tspan5. 630× magnifications, scale bar: 10 μm. Quantification of the protein expression was performed by Zeiss zen microscope software. Mean ± SD, n = 9, Student's t-test; **P < 0.01. (D) Representative IHC images of Tspan5, E-cadherin and vimentin expressions in xenograft sections of metastatic foci in mouse lungs. 600× magnifications, scale bar: 50 μm. Mean ± SD,

Application: IF/ICC Species: Human Sample: HCC cells

8). Entrectinib ameliorates bleomycin-induced pulmonary fibrosis in mice by inhibiting TGF-β1 signaling pathway. International Immunopharmacology, 2022 (PubMed: 36375321) [IF=4.8]

9). Neuron Specific Enolase Promotes Metastasis by Activating the Wnt/β-catenin Pathway in Small Cell Lung Cancer. Translational Oncology, 2020 (PubMed: 33618068) [IF=4.5]

Application: WB Species: human Sample: SCLC cells

Fig. 3.| NSE induced EMT in SCLC cells. (A, B) NSE overexpression promoted the EMT process of SCLC. (A) Western blot assay was performed to measure the protein expression levels of the EMT-related markers (Snail, N-cadherin and E-cadherin).

Application: WB Species: Human Sample: SCLC cells

Fig. 3 NSE induced EMT in SCLC cells. (A, B) NSE overexpression promoted the EMT process of SCLC. (A) Western blot assay was performed to measure the protein expression levels of the EMT-related markers (Snail, N-cadherin and E-cadherin). (B) qRT-PCR was performed to measure the mRNA expression levels of the EMT markers. (C, D) NSE knockdown represses the EMT process of SCLC. Protein levels (C) and mRNA levels (D) of EMT-related markers were measured using western blot or qRT-PCR, respectively. These results were repeated of three independent experiments.

10). Neuron specific enolase promotes tumor metastasis by activating the Wnt/β-catenin pathway in small cell lung cancer. Translational Oncology, 2021 (PubMed: 33618068) [IF=4.5]

Application: WB Species: Human Sample: sclc cells

Fig. 3. NSE induced EMT in SCLC cells. (A, B) NSE overexpression promoted the EMT process of SCLC. (A) Western blot assay was performed to measure the protein expression levels of the EMT-related markers (Snail, N-cadherin and E-cadherin). (B) qRT-PCR was performed to measure the mRNA expression levels of the EMT markers. (C, D) NSE knockdown represses the EMT process of SCLC. Protein levels (C) and mRNA levels (D) of EMT-related markers were measured using western blot or qRT-PCR, respectively. These results were repeated of three independent experiments.

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N Cadherin Antibody - #AF4039