SGTA Mouse Monoclonal Antibody | Affinity Biosciences

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SGTA Mouse Monoclonal Antibody - Western blot analysis of extracts from Mouse brain, using SGTA Mouse Monoclonal Antibody at 1/1000 dilution.

SGTA Mouse Monoclonal Antibody - Western blot analysis of extracts from Rat brain, using SGTA Mouse Monoclonal Antibody at 1/1000 dilution.

SGTA Mouse Monoclonal Antibody - Western blot analysis of extracts from Mouse spleen, using SGTA Mouse Monoclonal Antibody at 1/1000 dilution.

SGTA Mouse Monoclonal Antibody - Western blot analysis of extracts from Rat lung, using SGTA Mouse Monoclonal Antibody at 1/1000 dilution.

SGTA Mouse Monoclonal Antibody - Western blot analysis of extracts from Rat testis, using SGTA Mouse Monoclonal Antibody at 1/1000 dilution.

SGTA Mouse Monoclonal Antibody - Western blot analysis of extracts from PC12 cells(LPS 4h treatment), using SGTA Mouse Monoclonal Antibody at 1/1000 dilution.

SGTA Mouse Monoclonal Antibody - Western blot analysis of extracts from Mouse lung, using SGTA Mouse Monoclonal Antibody at 1/1000 dilution.

SGTA Mouse Monoclonal Antibody - Western blot analysis of extracts from A549 cells(heat-shock treatment), using SGTA Mouse Monoclonal Antibody at 1/1000 dilution.

SGTA Mouse Monoclonal Antibody - BF8384 at 1/100 staining human gastric cancer by IHC-P.

Product:	SGTA Mouse Monoclonal Antibody
Catalog:	BF8384
Description:	Mouse monoclonal antibody to SGTA
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	34kDa; 34kD(Calculated).
Uniprot:	O43765

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Monoclonal [AFfirm8384]

Specificity:

SGTA Mouse Monoclonal Antibody detects endogenous levels of total SGTA

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Alpha SGT; Alpha-SGT; AlphaSGT; hSGT; SGT; Sgt1; SGTA; SGTA_HUMAN; Small glutamine rich protein with tetratricopeptide repeats 1; Small glutamine rich tetratricopeptide repeat containing protein alpha; Small glutamine rich tetratricopeptide repeat TPR containing alpha; Small glutamine rich tetratricopeptide repeat-containing protein; Small glutamine-rich tetratricopeptide repeat-containing protein alpha; Stg; UBP; Vpu binding protein; Vpu-binding protein;

Immunogens

Immunogen:

Uniprot:

O43765(SGTA_HUMAN) >>Visit HPA database.

Gene(ID):

SGTA(6449)

Expression:

O43765 SGTA_HUMAN:

Ubiquitous.

Sequence:

O43765 SGTA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKEMPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIELNPANAVYFCNRAAAYSKLGNYAGAVQDCERAICIDPAYSKAYGRMGLALSSLNKHVEAVAYYKKALELDPDNETYKSNLKIAELKLREAPSPTGGVGSFDIAGLLNNPGFMSMASNLMNNPQIQQLMSGMISGGNNPLGTPGTSPSQNDLASLIQAGQQFAQQMQQQNPELIEQLRSQIRSRTPSASNDDQQE

PTMs - O43765 As Substrate

O43765

Site	PTM Type	Source
K4	Acetylation	Uniprot
Y9	Phosphorylation	Uniprot
R76	Methylation	Uniprot
S77	Phosphorylation	Uniprot
T81	Phosphorylation	Uniprot
S84	Phosphorylation	Uniprot
K95	Ubiquitination	Uniprot
K103	Ubiquitination	Uniprot
K116	Ubiquitination	Uniprot
C129	S-Nitrosylation	Uniprot
K137	Acetylation	Uniprot
K137	Ubiquitination	Uniprot
Y141	Phosphorylation	Uniprot
C148	S-Nitrosylation	Uniprot
C153	S-Nitrosylation	Uniprot
Y158	Phosphorylation	Uniprot
S159	Phosphorylation	Uniprot
K160	Acetylation	Uniprot
K160	Ubiquitination	Uniprot
Y162	Phosphorylation	Uniprot
S171	Phosphorylation	Uniprot
K174	Ubiquitination	Uniprot
K183	Ubiquitination	Uniprot
K184	Ubiquitination	Uniprot
K196	Ubiquitination	Uniprot
K200	Ubiquitination	Uniprot
K205	Ubiquitination	Uniprot
S297	Phosphorylation	Uniprot
S301	Phosphorylation	Uniprot
T303	Phosphorylation	Uniprot
S305	Phosphorylation	Uniprot
S307	Phosphorylation	Uniprot

Research Backgrounds

O43765_SGTA_HUMAN

Function:

Co-chaperone that binds misfolded and hydrophobic patches-containing client proteins in the cytosol. Mediates their targeting to the endoplasmic reticulum but also regulates their sorting to the proteasome when targeting fails. Functions in tail-anchored/type II transmembrane proteins membrane insertion constituting with ASNA1 and the BAG6 complex a targeting module. Functions upstream of the BAG6 complex and ASNA1, binding more rapidly the transmembrane domain of newly synthesized proteins. It is also involved in the regulation of the endoplasmic reticulum-associated misfolded protein catabolic process via its interaction with BAG6: collaborates with the BAG6 complex to maintain hydrophobic substrates in non-ubiquitinated states. Competes with RNF126 for interaction with BAG6, preventing the ubiquitination of client proteins associated with the BAG6 complex. Binds directly to HSC70 and HSP70 and regulates their ATPase activity.

(Microbial infection) In case of infection by polyomavirus, involved in the virus endoplasmic reticulum membrane penetration and infection via interaction with DNAJB12, DNAJB14 and HSPA8/Hsc70.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Co-localizes with HSP90AB1 in the cytoplasm. Increased nuclear accumulation seen during cell apoptosis.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer. Homooligomer (By similarity). Interacts with DNAJC5 and DNAJC5B. Interacts (via TPR repeats) with HSP90AA1. Interacts (via Gln-rich region) with SLC2A1. Interacts with HSP90AB1. Interacts (via TPR repeats) with HSPA8/Hsc70; the interaction is direct. Interacts with BAG6 (via ubiquitin-like domain); interaction prevents interaction between BAG6 and RNF126. Forms a multiprotein complex, at least composed of DNAJB12, DNAJB14, HSPA8/Hsc70 and SGTA; interaction with DNAJB14 and HSPA8/Hsc70 is direct.

(Microbial infection) Interacts with Vpu and Gag from HIV-1.

(Microbial infection) Interacts with SARS-CoV accessory protein 7a.

Family&Domains:

The second tetratricopeptide repeat (TPR 2) mediates the interaction with SARS-CoV accessory protein 7a.

Belongs to the SGT family.

Restrictive clause

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For Research Use Only.
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AFfirm™ SGTA Mouse Monoclonal Antibody - #BF8384