FOXP4 Antibody - #AF4057
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| Product: | FOXP4 Antibody |
| Catalog: | AF4057 |
| Description: | Rabbit polyclonal antibody to FOXP4 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 74kd; 73kD(Calculated). |
| Uniprot: | Q8IVH2 |
| RRID: | AB_2835349 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
FOXP4 Antibody detects endogenous levels of total FOXP4.
RRID:
AB_2835349
Cite Format: Affinity Biosciences Cat# AF4057, RRID:AB_2835349.
Cite Format: Affinity Biosciences Cat# AF4057, RRID:AB_2835349.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
1200010K03Rik; 2310007G05Rik; FKHLA; FLJ40908; FLJ44184; Fork head related protein like A; Fork head-related protein-like A; Forkhead box P4; Forkhead box protein P4; FOX P4; FOXP 4; foxp4; FOXP4_HUMAN; hFKHLA; mFKHLA; OTTHUMP00000016374; OTTHUMP00000039784; OTTHUMP00000043412; OTTHUMP00000043536; Winged helix repressor FOX P4; Winged helix repressor FOXP 4; Winged helix repressor FOXP4;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Description:
Transcriptional repressor that represses lung-specific expression.
Sequence:
- Q8IVH2 FOXP4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMVESASETIRSAPSGQNGVGSLSGQADGSSGGATGTTASGTGREVTTGADSNGEMSPAELLHFQQQQALQVARQFLLQQASGLSSPGNNDSKQSASAVQVPVSVAMMSPQMLTPQQMQQILSPPQLQALLQQQQALMLQQLQEYYKKQQEQLHLQLLTQQQAGKPQPKEALGNKQLAFQQQLLQMQQLQQQHLLNLQRQGLVSLQPNQASGPLQTLPQAAVCPTDLPQLWKGEGAPGQPAEDSVKQEGLDLTGTAATATSFAAPPKVSPPLSHHTLPNGQPTVLTSRRDSSSHEETPGSHPLYGHGECKWPGCETLCEDLGQFIKHLNTEHALDDRSTAQCRVQMQVVQQLEIQLAKESERLQAMMAHLHMRPSEPKPFSQPLNPVPGSSSFSKVTVSAADSFPDGLVHPPTSAAAPVTPLRPPGLGSASLHGGGPARRRSSDKFCSPISSELAQNHEFYKNADVRPPFTYASLIRQAILETPDRQLTLNEIYNWFTRMFAYFRRNTATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEREYQKRRPPKMTGSPTLVKNMISGLSYGALNASYQAALAESSFPLLNSPGMLNPGSASSLLPLSHDDVGAPVEPLPSNGSSSPPRLSPPQYSHQVQVKEEPAEAEEDRQPGPPLGAPNPSASGPPEDRDLEEELPGEELS
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
58
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IVH2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S52 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K175 | Acetylation | Uniprot | |
| K175 | Sumoylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K232 | Sumoylation | Uniprot | |
| K246 | Sumoylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| T297 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| S442 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| T556 | Phosphorylation | Uniprot | |
| S622 | Phosphorylation | Uniprot | |
| S627 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| S662 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Transcriptional repressor that represses lung-specific expression.
Subcellular Location:
Nucleus.
Subunit Structure:
Forms homodimers and heterodimers with FOXP1 and FOXP2. Dimerization is required for DNA-binding (By similarity).
Family&Domains:
The leucine-zipper is required for dimerization and transcriptional repression.
References
1). Downregulation of microRNA‑423‑5p suppresses TGF‑β1‑induced EMT by targeting FOXP4 in airway fibrosis. Molecular Medicine Reports, 2022
(PubMed: 35642665)
[IF=3.4]
Application: IF/ICC Species: Sample:
Figure 3. Identification of target genes of miR‑423‑5p. (A‑C) The location of miR‑423‑5p and FOXP4 mRNA in BEAS‑2B cells was determined by fluores‑
cence in situ hybridization (scale bar=200 µm). (D) The binding of miR‑423‑5p and FOXP4 in BEAS‑2B cells was detected by an RNA pull‑down assay.
(E) The expression of FOXP4 in AF tissues and TGF‑β1‑stimulated BEAS‑2B cells was detected by immunohistochemistry (scale bar=50 µm) and RT‑qPCR.
The relative expression of FOXP4 in BEAS‑2B cells transfected with a miR‑423‑5p mimic or inhibitor for 24 h and then treated with TGF‑β1 for 24 h was
determined by (G) RT‑qPCR, (F) immunofluorescence staining (scale bar=50 µm.) and (H) western blot analysis. *
P<0.05 and **P<0.01. miR, microRNA;
FOXP4, forkhead box p4; RT‑qPCR, reverse transcription‑quantitative PCR; AF, airway fibrosis.
Application: WB Species: Human Sample: BEAS‑2B cell
Figure 3. Identification of target genes of miR‑423‑5p. (A‑C) The location of miR‑423‑5p and FOXP4 mRNA in BEAS‑2B cells was determined by fluores‑
cence in situ hybridization (scale bar=200 µm). (D) The binding of miR‑423‑5p and FOXP4 in BEAS‑2B cells was detected by an RNA pull‑down assay.
(E) The expression of FOXP4 in AF tissues and TGF‑β1‑stimulated BEAS‑2B cells was detected by immunohistochemistry (scale bar=50 µm) and RT‑qPCR.
The relative expression of FOXP4 in BEAS‑2B cells transfected with a miR‑423‑5p mimic or inhibitor for 24 h and then treated with TGF‑β1 for 24 h was
determined by (G) RT‑qPCR, (F) immunofluorescence staining (scale bar=50 µm.) and (H) western blot analysis. *
P<0.05 and **P<0.01. miR, microRNA;
FOXP4, forkhead box p4; RT‑qPCR, reverse transcription‑quantitative PCR; AF, airway fibrosis.
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