HMGA2 Antibody - #AF4069

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Product: HMGA2 Antibody
Catalog: AF4069
Description: Rabbit polyclonal antibody to HMGA2
Application: WB IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep
Mol.Wt.: 18kd; 12kD(Calculated).
Uniprot: P52926
RRID: AB_2835351

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 50ul $250 In stock
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 200ul $450 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(80%), Bovine(100%), Sheep(100%)
Clonality:
Polyclonal
Specificity:
HMGA2 Antibody detects endogenous levels of total HMGA2.
RRID:
AB_2835351
Cite Format: Affinity Biosciences Cat# AF4069, RRID:AB_2835351.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HMGA 2; BABL; High mobility group (nonhistone chromosomal) protein isoform I C; High mobility group (nonhistone chromosomal) protein isoform IC; High Mobility Group AT hook 2; High Mobility Group AT hook protein 2; High mobility group AT-hook protein 2; High mobility group protein HMGI C; High mobility group protein HMGI-C; High mobility group protein HMGIC; High mobility group protein I, isoform C; HMGA2; HMGA2_HUMAN; HMGI C; HMGIC; LIPO; Non histone chromosomal architectural protein HMGI C; Non histone chromosomal architectural protein HMGIC; Pygmy; STQTL9;

Immunogens

Immunogen:

A synthesized peptide derived from human HMGA2, corresponding to a region within C-terminal amino acids.

Uniprot:

P52926(HMGA2_HUMAN) >>Visit HPA database.

Gene(ID):
HMGA2(8091)
Description:
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
Sequence:
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWPQQVVQKKPAQEETEETSSQESAEED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Pig
80
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation (By similarity).

PTMs:

Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. Phosphorylated by NEK2 (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the HMGA family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

References

1). CircFBXL5 promotes the 5-FU resistance of breast cancer via modulating miR-216b/HMGA2 axis. Cancer Cell International, 2021 (PubMed: 34281530) [IF=5.8]

Application: WB    Species: Human    Sample: MDA-MB-453, MDA-MB-231 and MCF-10A cells

Fig. 5 The relationship of miR-216b and HMGA2. A The binding sequence of miR-216b and HMGA2. B, C Luciferase activity was examined in cells with co-transfection of HMGA2-WT or HMGA2-MUT and miR-216b mimic or miR-con. D HMGA2 abundance in cells with transfection of biotin-miR-con or biotin-miR-216b after RNA pull-down. E HMGA2 protein expression in MDA-MB-453, MDA-MB-231 and MCF-10A cells. F HMGA2 abundance in 5-FU-resistant and sensitive MDA-MB-231 and MDA-MB-453 cells. G, H HMGA2 protein level in cells with transfection of miR-con, miR-216b mimic, in-miR-con or in-miR-216b. Mock: non-transfected group. * P < 0.05
2). Long non-coding RNA LINC00460 promotes proliferation and inhibits apoptosis of cervical cancer cells by targeting microRNA-503-5p. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2020 (PubMed: 32740791) [IF=3.5]

Application: WB    Species: human    Sample: SiHa and HeLa cells

Fig. 4 | LINC00460 acts as a competing endogenous RNA of miR503-5p in SiHa and HeLa cells.d–h Expressions of AKT2,HMGA2 and SHOX2 in SiHa and HeLa cells by western blotting.**p<0.01 versus cells co-transfected with wild-type LINC00460 and miR-503-5p mimic. ##p<0.01 versus cells transfected with NC shRNA. WT wild-type LINC00460, MUT mutated LINC00460

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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