CNPase Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

Western blot analysis of extracts from VERO, using CNPase Antibody.

AF4084 at 1/100 staining Mouse brain tissue by IHC-P.

AF4084 at 1/100 staining Rat brain tissue by IHC-P.

AF4084 staining Hela cells by IF/ICC.

Product:	CNPase Antibody
Catalog:	AF4084
Description:	Rabbit polyclonal antibody to CNPase
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	47kd; 48kD(Calculated).
Uniprot:	P09543
RRID:	AB_2835363

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Monkey

Prediction:

Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Dog(83%)

Clonality:

Polyclonal

Specificity:

CNPase Antibody detects endogenous levels of total CNPase.

RRID:

AB_2835363
Cite Format: Affinity Biosciences Cat# AF4084, RRID:AB_2835363.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2''; 2'3' cyclic nucleotide 3' phosphodiesterase; 3''-cyclic-nucleotide 3''-phosphodiesterase; CN37_HUMAN; CNP 1; CNP; CNP1; CNPase;

Immunogens

Immunogen:

Uniprot:

P09543(CN37_HUMAN) >>Visit HPA database.

Gene(ID):

CNP(1267)

Description:

Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 2'-phosphate.

Sequence:

P09543 CN37_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNRGFSRKSHTFLPKIFFRKMSSSGAKDKPELQFPFLQDEDTVATLLECKTLFILRGLPGSGKSTLARVIVDKYRDGTKMVSADAYKITPGARGAFSEEYKRLDEDLAAYCRRRDIRILVLDDTNHERERLEQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSKAFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTII

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Chicken

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09543 As Substrate

P09543

Site	PTM Type	Source
S6	Phosphorylation	Uniprot
K8	Ubiquitination	Uniprot
S9	Phosphorylation	Uniprot
T11	Phosphorylation	Uniprot
K15	Ubiquitination	Uniprot
S22	Phosphorylation	Uniprot
S24	Phosphorylation	Uniprot
K29	Ubiquitination	Uniprot
K63	Ubiquitination	Uniprot
K73	Ubiquitination	Uniprot
K79	Ubiquitination	Uniprot
Y86	Phosphorylation	Uniprot
K87	Ubiquitination	Uniprot
Y100	Phosphorylation	Uniprot
K101	Ubiquitination	Uniprot
Y110	Phosphorylation	Uniprot
C111	S-Nitrosylation	Uniprot
Y141	Phosphorylation	Uniprot
K162	Ubiquitination	Uniprot
K164	Ubiquitination	Uniprot
S170	Phosphorylation	Uniprot
K175	Acetylation	Uniprot
K175	Ubiquitination	Uniprot
K176	Ubiquitination	Uniprot
K204	Ubiquitination	Uniprot
K217	Ubiquitination	Uniprot
K220	Ubiquitination	Uniprot
K235	Ubiquitination	Uniprot
Y241	Phosphorylation	Uniprot
K244	Ubiquitination	Uniprot
K255	Ubiquitination	Uniprot
K261	Ubiquitination	Uniprot
K275	Ubiquitination	Uniprot
T292	Phosphorylation	Uniprot
S312	Phosphorylation	Uniprot
K316	Ubiquitination	Uniprot
S318	Phosphorylation	Uniprot
K356	Ubiquitination	Uniprot
K371	Ubiquitination	Uniprot
Y373	Phosphorylation	Uniprot
T383	Phosphorylation	Uniprot
T406	Phosphorylation	Uniprot
S409	Phosphorylation	Uniprot

Research Backgrounds

P09543_CN37_HUMAN

Function:

May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.

Subcellular Location:

Membrane>Lipid-anchor. Melanosome.
Note: Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Subunit Structure:

Exists as monomers and homodimers.

Family&Domains:

Belongs to the 2H phosphoesterase superfamily. CNPase family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CNPase Antibody - #AF4084