Product: CNPase Antibody
Catalog: AF4084
Description: Rabbit polyclonal antibody to CNPase
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 47kd; 48kD(Calculated).
Uniprot: P09543
RRID: AB_2835363

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Dog(83%)
Clonality:
Polyclonal
Specificity:
CNPase Antibody detects endogenous levels of total CNPase.
RRID:
AB_2835363
Cite Format: Affinity Biosciences Cat# AF4084, RRID:AB_2835363.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2''; 2'3' cyclic nucleotide 3' phosphodiesterase; 3''-cyclic-nucleotide 3''-phosphodiesterase; CN37_HUMAN; CNP 1; CNP; CNP1; CNPase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 2'-phosphate.
Sequence:
MNRGFSRKSHTFLPKIFFRKMSSSGAKDKPELQFPFLQDEDTVATLLECKTLFILRGLPGSGKSTLARVIVDKYRDGTKMVSADAYKITPGARGAFSEEYKRLDEDLAAYCRRRDIRILVLDDTNHERERLEQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSKAFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTII

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
83
Horse
83
Bovine
83
Sheep
83
Dog
83
Rabbit
75
Chicken
67
Zebrafish
50
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09543 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
K8 Ubiquitination
S9 Phosphorylation
T11 Phosphorylation
K15 Ubiquitination
S22 Phosphorylation
S24 Phosphorylation
K29 Ubiquitination
K63 Ubiquitination
K73 Ubiquitination
K79 Ubiquitination
Y86 Phosphorylation
K87 Ubiquitination
Y100 Phosphorylation
K101 Ubiquitination
Y110 Phosphorylation
C111 S-Nitrosylation
Y141 Phosphorylation
K162 Ubiquitination
K164 Ubiquitination
S170 Phosphorylation
K175 Acetylation
K175 Ubiquitination
K176 Ubiquitination
K204 Ubiquitination
K217 Ubiquitination
K220 Ubiquitination
K235 Ubiquitination
Y241 Phosphorylation
K244 Ubiquitination
K255 Ubiquitination
K261 Ubiquitination
K275 Ubiquitination
T292 Phosphorylation
S312 Phosphorylation
K316 Ubiquitination
S318 Phosphorylation
K356 Ubiquitination
K371 Ubiquitination
Y373 Phosphorylation
T383 Phosphorylation
T406 Phosphorylation
S409 Phosphorylation

Research Backgrounds

Function:

May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.

Subcellular Location:

Membrane>Lipid-anchor. Melanosome.
Note: Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Exists as monomers and homodimers.

Family&Domains:

Belongs to the 2H phosphoesterase superfamily. CNPase family.

Restrictive clause

 

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For Research Use Only.
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