DDX4 Antibody - #AF4098
| Product: | DDX4 Antibody |
| Catalog: | AF4098 |
| Description: | Rabbit polyclonal antibody to DDX4 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Dog |
| Mol.Wt.: | 80kd; 79kD(Calculated). |
| Uniprot: | Q9NQI0 |
| RRID: | AB_2835367 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF4098, RRID:AB_2835367.
Fold/Unfold
DDX 4; Ddx4; DDX4_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 4; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MVH; Probable ATP dependent RNA helicase DDX4; Probable ATP-dependent RNA helicase DDX4; VASA; Vasa homolog;
Immunogens
Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
- Q9NQI0 DDX4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDAGECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGGYRDGNNSEASGPYRRGGRGSFRGCRGGFGLGSPNNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NQI0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T37 | Phosphorylation | Uniprot | |
| K75 | Acetylation | Uniprot | |
| K89 | Acetylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| Y133 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| T312 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| T339 | Phosphorylation | Uniprot | |
| Y400 | Phosphorylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| K432 | Acetylation | Uniprot | |
| Y442 | Phosphorylation | Uniprot | |
| K530 | Acetylation | Uniprot |
Research Backgrounds
ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity). Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity).
Cytoplasm. Cytoplasm>Perinuclear region.
Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4 (By similarity). Interacts with RANBP9. Interacts with RANBP10. Interacts with PIWIL2 and MAEL. Interacts with ARNTL/BMAL1 and CLOCK. Interacts with Tex19.1 and, probably, Tex19.2 (By similarity).
Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
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