Product: DLGAP1 Antibody
Catalog: AF0308
Description: Rabbit polyclonal antibody to DLGAP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 108kDa; 109kD(Calculated).
Uniprot: O14490
RRID: AB_2833472

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DLGAP1 Antibody detects endogenous levels of total DLGAP1.
RRID:
AB_2833472
Cite Format: Affinity Biosciences Cat# AF0308, RRID:AB_2833472.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

4933422O14Rik; 9630002F18; AI845682; AI848168; BB075781; D17Bwg0511e; DAP 1 ALPHA; DAP 1; DAP 1 BETA; DAP-1; DAP-1-ALPHA; DAP-1-BETA; DAP1; Discs large (Drosophila) homolog associated protein 1; Discs large homolog associated protein 1; Disks large associated protein 1; Disks large associated protein 1, alpha isoform, included; Disks large associated protein 1, beta isoform, included; Disks large-associated protein 1; DLGAP 1; Dlgap1; DLGP1_HUMAN; GKAP; GKAP/SAPAP; GKPA/SAPAP; Guanylate kinase associated protein; Guanylate kinase-associated protein; hGKAP; KIAA4162; MGC88156; mKIAA4162; PSD 95/SAP90 binding protein 1; PSD-95/SAP90-binding protein 1; SAP90/PSD 95 associated protein 1; SAP90/PSD-95-associated protein 1; SAPAP 1; SAPAP1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O14490 DLGP1_HUMAN:

Expressed in brain.

Description:
SAPAP1 Part of the postsynaptic scaffold in neuronal cells. Belongs to the SAPAP family. Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN.
Sequence:
MKGLSGSRSHHHGVTCDSACDSLSHHSDRKPYLLSPVEHHPADHPYYTQRNSFQAECVGPFSDPLASSTFPRRHYTSQQELKDECALVPRTLATKANRIPANLLDQFERQLPLSRDGYHTLQYKRTAVEHRSDSPGRIRHLVHSVQKLFTKSHSLEGPSKGSVNGGKASPDEAQAARYGKRSKSKERRAEPKARPSTSPGWWSSDDNLDGDMCIYHAPSGVMTMGRCPDRSASQYFLEAYNTISEQAVKASRSNNDVKCSTCANLPVSLDTPLLKKSAWSSTLTVSRAREVYQKASVNMDQAMVKSESCQQERSCQYLQVPQDEWTGYTPRGKDDEIPCRRMRSGSYIKAMGDEDSGDSDTSPKPSPKVAARRESYLKATQPSLTELTTLKISNEHSPKLQIRSHSYLRAVSEVSINRSLDSLDPAGLLTSPKFRSRNESYMRAMSTISQVSEMEVNGQFESVCESVFSELESQAVEALDLPMPGCFRMRSHSYVRAIEKGCSQDDECVSLRSSSPPRTTTTVRTIQSSTVSSCITTYKKTPPPVPPRTTTKPFISITAQSSTESAQDAYMDGQGQRGDIISQSGLSNSTESLDSMKALTAAIEAANAQIHGPASQHMGNNTATVTTTTTIATVTTEDRKKDHFKKNRCLSIGIQVDDAEEPDKTGENKAPSKFQSVGVQVEEEKCFRRFTRSNSVTTAVQADLDFHDNLENSLESIEDNSCPGPMARQFSRDASTSTVSIQGSGNHYHACAADDDFDTDFDPSILPPPDPWIDSITEDPLEAVQRSVCHRDGHWFLKLLQAERDRMEGWCQQMEREERENNLPEDILGKIRTAVGSAQLLMAQKFYQFRELCEENLNPNAHPRPTSQDLAGFWDMLQLSIENISMKFDELHQLKANNWKQMDPLDKKERRAPPPVPKKPAKGPAPLIRERSLESSQRQEARKRLMAAKRAASVRQNSATESAESIEIYIPEAQTRL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
71
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14490 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
S24 Phosphorylation
S52 Phosphorylation
Y118 Phosphorylation
S154 Phosphorylation
S169 Phosphorylation
S314 Phosphorylation
Y317 Phosphorylation
S346 Phosphorylation
S356 Phosphorylation
S359 Phosphorylation
S375 Phosphorylation
S383 Phosphorylation
S397 Phosphorylation
S406 Phosphorylation
S412 Phosphorylation
S415 Phosphorylation
S419 Phosphorylation
S422 Phosphorylation
S440 Phosphorylation
S491 Phosphorylation
S493 Phosphorylation
K500 Ubiquitination
S503 Phosphorylation
S510 Phosphorylation
T530 Phosphorylation
T536 Phosphorylation
T537 Phosphorylation
T541 Phosphorylation
S589 Phosphorylation
S592 Phosphorylation
S595 Phosphorylation
S651 Phosphorylation
K673 Acetylation
S958 Phosphorylation

Research Backgrounds

Function:

Part of the postsynaptic scaffold in neuronal cells.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Cell junction>Synapse>Postsynaptic density. Cell junction>Synapse.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain.

Subunit Structure:

Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1 (By similarity). Interacts with MPP2 (via the SH3-Guanylate kinase-like sub-module) (By similarity).

Family&Domains:

Belongs to the SAPAP family.

Research Fields

· Organismal Systems > Nervous system > Glutamatergic synapse.

References

1). Liang D et al. Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing. Nature Communications 2021 Jan 12;12(1):287. (PubMed: 33436583) [IF=16.6]

Application: IF/ICC    Species: mouse    Sample: heart

Fig. 2| Expression pattern of the core cluster markers Vsnl1, Dlgap1, and Unc80 in mouse heart.f Immunofluorescence images of HCN4(green), DLGAP1 (red), and DAPI (blue).

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