AFfirm™ RAN Mouse Monoclonal Antibody - #BF9302

Product: RAN Mouse Monoclonal Antibody
Catalog: BF9302
Description: Mouse monoclonal antibody to RAN
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Pig, Zebrafish, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 26 kDa; 24kD(Calculated).
Uniprot: P62826

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm9302]
Specificity:
RAN Mouse Monoclonal Antibody detects endogenous levels of total RAN.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Androgen receptor associated protein 24; Androgen receptor-associated protein 24; ARA 24; ARA24; Gsp1; GTP binding nuclear protein RAN; GTP-binding nuclear protein Ran; GTPase Ran; Guanosine triphosphatase Ran; LPS; OK/SW-cl.81; ran; RAN member RAS oncogene family; RAN_HUMAN; RanGTPase; Ras like protein TC4; Ras related nuclear protein; Ras-like protein TC4; Ras-related nuclear protein; RASL2 8; TC 4; TC4;

Immunogens

Immunogen:

A synthesized peptide derived from human RAN, corresponding to a region within C-terminal amino acids.

Uniprot:

P62826(RAN_HUMAN) >>Visit HPA database.

Gene(ID):
RAN(5901)
Expression:
P62826 RAN_HUMAN:

Expressed in a variety of tissues.

Description:
GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Sequence:
MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL

Research Backgrounds

Function:

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.

PTMs:

Acetylation by KAT5 at Lys-134 is increased during mitosis, impairs RANGRF binding and enhances RCC1 binding. Acetylation at Lys-37 enhances the association with nuclear export components. Deacetylation of Lys-37 by SIRT7 regulates the nuclear export of NF-kappa-B subunit RELA/p65.

Subcellular Location:

Nucleus. Nucleus envelope. Cytoplasm>Cytosol. Cytoplasm. Melanosome.
Note: Predominantly nuclear during interphase (PubMed:8421051, PubMed:12194828, PubMed:10679025). Becomes dispersed throughout the cytoplasm during mitosis (PubMed:8421051, PubMed:12194828). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a variety of tissues.

Family&Domains:

Belongs to the small GTPase superfamily. Ran family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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For Research Use Only.
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