AFfirm™ WTAP Mouse Monoclonal Antibody - #BF9330

Product: WTAP Mouse Monoclonal Antibody
Catalog: BF9330
Description: Mouse monoclonal antibody to WTAP
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 44 KD; 44kD(Calculated).
Uniprot: Q15007

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm9330]
Specificity:
WTAP Mouse Monoclonal Antibody detects endogenous levels of total WTAP.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686F20131; Female-lethal(2)D homolog; FL2D_HUMAN; hFL(2)D; KIAA0105; MGC3925; Mum2; OTTHUMP00000017522; OTTHUMP00000017523; PNAS 132; PNAS-132; PNAS132; Pre mRNA splicing regulator WTAP; Pre-mRNA-splicing regulator WTAP; Putative pre mRNA splicing regulator female lethal 2D homolog; Putative pre mRNA splicing regulator female lethal(2D); Putative pre-mRNA splicing regulator female lethal(2D) homolog; putative pre-mRNA splicing regulator female-lethal(2D); Wilms tumor 1 associated protein; Wilms tumor 1 associating protein; Wilms tumor 1-associating protein; Wilms' tumor 1 associating protein; Wilms' tumour 1-associating protein; WT1 associated protein; WT1-associated protein; WT1-associating protein; wtap;

Immunogens

Immunogen:
Uniprot:

Q15007(FL2D_HUMAN) >>Visit HPA database.

Gene(ID):
WTAP(9589)
Expression:
Q15007 FL2D_HUMAN:

Ubiquitously expressed.

Sequence:
MTNEEPLPKKVRLSETDFKVMARDELILRWKQYEAYVQALEGKYTDLNSNDVTGLRESEEKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQLKETRQQLAQYQQQQSQASAPSTSRTTASEPVEQSEATSKDCSRLTNGPSNGSSSRQRTSGSGFHREGNTTEDDFPSSPGNGNKSSNSSEERTGRGGSGYVNQLSAGYESVDSPTGSENSLTHQSNDTDSSHDPQEEKAVSGKGNRTVGSRHVQNGLDSSVNVQGSVL

PTMs - Q15007 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T2 Phosphorylation
K10 Ubiquitination
S14 Phosphorylation
K19 Ubiquitination
K31 Ubiquitination
K43 Ubiquitination
Y44 Phosphorylation
S49 Phosphorylation
S58 Phosphorylation
K61 Ubiquitination
K63 Ubiquitination
S69 Phosphorylation
K83 Ubiquitination
K98 Ubiquitination
K125 Sumoylation
K125 Ubiquitination
K134 Ubiquitination
K146 Ubiquitination
T148 Phosphorylation
K191 Ubiquitination
S244 Phosphorylation
S267 Phosphorylation
C270 S-Nitrosylation
T274 Phosphorylation
S288 Phosphorylation
S290 Phosphorylation
T298 Phosphorylation
T299 Phosphorylation
S305 Phosphorylation
S306 Phosphorylation
S313 Phosphorylation
S314 Phosphorylation
S316 Phosphorylation
S317 Phosphorylation
S341 Phosphorylation
T343 Phosphorylation
S345 Phosphorylation
S348 Phosphorylation
S388 Phosphorylation

Research Backgrounds

Function:

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.

Subcellular Location:

Nucleus speckle. Nucleus>Nucleoplasm. Cytoplasm.
Note: Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM. The MAC subcomplex is composed of METTL3 and METTL14. The MACOM subcomplex is composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, and, in some cases of RBM15 (RBM15 or RBM15B). Interacts with WT1. Also component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, VIRMA, RBM15, BCLAF1 and THRAP3.

Family&Domains:

Belongs to the fl(2)d family.

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