AFfirm™ NQO1 Mouse Monoclonal Antibody - #BF9386

Product: NQO1 Mouse Monoclonal Antibody
Catalog: BF9386
Description: Mouse monoclonal antibody to NQO1
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 31kDa; 31kD(Calculated).
Uniprot: P15559

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm9386]
Specificity:
NQO1 Mouse Monoclonal Antibody detects endogenous levels of total NQO1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Azoreductase; Cytochrome b 5 reductase; DHQU; DIA 4; DIA4; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase); Diaphorase (NADH/NADPH); Diaphorase 4; Dioxin inducible 1; DT diaphorase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase quinone 1; NAD(P)H menadione oxidoreductase 1 dioxin inducible; NAD(P)H quinone dehydrogenase 1; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR 1; NMOR I; NMOR1; NMORI; NQO 1; NQO1; NQO1_HUMAN; Phylloquinone reductase; QR 1; QR1; Quinone reductase 1;

Immunogens

Immunogen:
Uniprot:

P15559(NQO1_HUMAN) >>Visit HPA database.

Gene(ID):
NQO1(1728)
Description:
NAD(P)H:quinone oxidoreductase 1 (NQO1) is a flavoprotein that catalyzes the two-electron reduction of quinones and their derivatives (1,2). This enzyme protects cells against redox cycling and oxidative stress (1,3). The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues (1,2). Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development (1). Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turns leads to myeloid hyperplasia of bone marrow (2).
Sequence:
MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK

PTMs - P15559 As Substrate

Site PTM Type Enzyme
S13 Phosphorylation
Y20 Phosphorylation
K23 Ubiquitination
K31 Acetylation
K31 Ubiquitination
K33 Ubiquitination
S40 Phosphorylation
Y43 Phosphorylation
K54 Ubiquitination
K59 Acetylation
K59 Ubiquitination
K61 Ubiquitination
Y68 Phosphorylation
Y76 Phosphorylation
K77 Acetylation
K77 Ubiquitination
S82 Phosphorylation
K90 Ubiquitination
K91 Ubiquitination
Y127 Phosphorylation
Y129 Phosphorylation
Y133 Phosphorylation
K135 Ubiquitination
K209 Acetylation
K209 Ubiquitination
K210 Acetylation
K210 Ubiquitination
K241 Ubiquitination
K248 Ubiquitination
K251 Sumoylation
K251 Ubiquitination
S255 Phosphorylation
K262 Acetylation
K262 Ubiquitination
K271 Sumoylation
K271 Ubiquitination

Research Backgrounds

Function:

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Interacts with PDLIM4 isoform 2; this interaction stabilizes PDLIM4 isoform 2 in response to oxidative stress and protects it from ubiquitin-independent degradation by the core 20S proteasome.

Family&Domains:

Belongs to the NAD(P)H dehydrogenase (quinone) family.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of cofactors and vitamins > Ubiquinone and other terpenoid-quinone biosynthesis.

Restrictive clause

 

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