AFfirm™ PPP1R14B Mouse Monoclonal Antibody - #BF9413

Product: PPP1R14B Mouse Monoclonal Antibody
Catalog: BF9413
Description: Mouse monoclonal antibody to PPP1R14B
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Pig, Horse, Rabbit, Dog
Mol.Wt.: 16 kDa; 16kD(Calculated).
Uniprot: Q96C90

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm9413]
Specificity:
PPP1R14B Mouse Monoclonal Antibody detects endogenous levels of total PPP1R14B.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PHI 1; PHI1; Phospholipase C beta 3 neighboring gene protein; Phospholipase C beta 3 neighbouring gene protein; Phospholipase C-beta-3 neighbouring gene protein; PLCB3N; PNG; PP14B_HUMAN; Ppp1r14b; Protein phosphatase 1 regulatory (inhibitor) subunit 14B; Protein phosphatase 1 regulatory subunit 14B; SOM172;

Immunogens

Immunogen:
Uniprot:

Q96C90(PP14B_HUMAN) >>Visit HPA database.

Gene(ID):
PPP1R14B(26472)
Expression:
Q96C90 PP14B_HUMAN:

Ubiquitous. Expressed at low levels.

Sequence:
MADSGTAGGAALAAPAPGPGSGGPGPRVYFQSPPGAAGEGPGGADDEGPVRRQGKVTVKYDRKELRKRLNLEEWILEQLTRLYDCQEEEIPELEIDVDELLDMESDDARAARVKELLVDCYKPTEAFISGLLDKIRGMQKLSTPQKK

PTMs - Q96C90 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S4 Phosphorylation
S21 Phosphorylation
R27 Methylation
Y29 Phosphorylation
S32 Phosphorylation
T57 Phosphorylation O75116 (ROCK2) , P17252 (PRKCA) , Q05655 (PRKCD) , Q13418 (ILK)
K59 Acetylation
K59 Methylation
K114 Ubiquitination
Y121 Phosphorylation
K122 Ubiquitination
S142 Phosphorylation
T143 Phosphorylation

Research Backgrounds

Function:

Inhibitor of PPP1CA. Has over 50-fold higher inhibitory activity when phosphorylated (By similarity).

PTMs:

Phosphorylated primarily on Thr-57 by PKC (in vitro). An unknown Ser is also phosphorylated by PKC (in vitro) (By similarity).

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Expressed at low levels.

Family&Domains:

Belongs to the PP1 inhibitor family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.