YAP Antibody - #DF3182

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Product: YAP Antibody
Catalog: DF3182
Description: Rabbit polyclonal antibody to YAP
Application: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 65~78kD; 54kD(Calculated).
Uniprot: P46937
RRID: AB_2835405

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
YAP Antibody detects endogenous levels of total YAP.
RRID:
AB_2835405
Cite Format: Affinity Biosciences Cat# DF3182, RRID:AB_2835405.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

65 kDa Yes associated protein; 65 kDa Yes-associated protein; COB1; YAp 1; YAP 65; YAP; YAP1; YAP1_HUMAN; YAP2; YAP65; yes -associated protein delta; Yes associated protein 1 65kDa; Yes associated protein 1; Yes associated protein 2; yes associated protein beta; YKI; Yorkie homolog;

Immunogens

Immunogen:

A synthesized peptide derived from human YAP, corresponding to a region within the internal amino acids.

Uniprot:

P46937(YAP1_HUMAN) >>Visit HPA database.

Gene(ID):
YAP1(10413)
Expression:
P46937 YAP1_HUMAN:

Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).

Sequence:
MDPGQQPPPQPAPQGQGQPPSQPPQGQGPPSGPGQPAPAATQAAPQAPPAGHQIVHVRGDSETDLEALFNAVMNPKTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLGAVSPGTLTPTGVVSGPAATPTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Bovine
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction.

Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).

PTMs:

Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage. Phosphorylation at Ser-400 and Ser-403 by CK1 is triggered by previous phosphorylation at Ser-397 by LATS proteins and leads to YAP1 ubiquitination by SCF(beta-TRCP) E3 ubiquitin ligase and subsequent degradation. Phosphorylated at Thr-119, Ser-138, Thr-154, Ser-367 and Thr-412 by MAPK8/JNK1 and MAPK9/JNK2, which is required for the regulation of apoptosis by YAP1.

Ubiquitinated by SCF(beta-TRCP) E3 ubiquitin ligase.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001). PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).

Family&Domains:

The first coiled-coil region mediates most of the interaction with TEAD transcription factors.

Belongs to the YAP1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

References

1). Engineered bone marrow mesenchymal stem cell-derived exosomes loaded with miR302 through the cardiomyocyte specific peptide can reduce myocardial ischemia and reperfusion (I/R) injury. Journal of translational medicine, 2024 (PubMed: 38368334) [IF=7.4]

Application: WB    Species: Rat    Sample: H9C2 cells

Fig. 5 The in vitro uptake and effect of DSPE-PEG-CMP-miR302-EXO on gene expression in H9C2 cells. A Fluorescence images indicated internalization of BMSCs-EVs and DSPE-PEG-CMP-miR302-EXO in H9C2 cells. Scale bar = 50 μm. B Quantitative polymerase chain reaction (qRT-PCR) indicated upregulation of miR302 level in H9C2 cells by DSPE-PEG-CMP-miR302-EXO. Relative values were presented as fold change over control. C The MTT assay showed increased proliferation ratio of H9C2 cells by DSPE-PEG-CMP-miR302-EXO. D–F Western blot analysis of Ki67 and Yap levels displayed increased levels of these proteins in cardiomyocytes after co-incubation with DSPE-PEG-CMP-miR302-EXO. GAPDH served as the internal reference. Relative values were presented as fold change over control. a: Control; b: Model; c: DSPE-PEG-CMP; d: DSPE-PEG-CMP-EXO; e: DSPE-PEG-CMP-miR302-EXO; f: miR302. B, E, F ▲▲p 
2). YAP1 protects against PM2.5-induced lung toxicity by suppressing pyroptosis and ferroptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2023 (PubMed: 36863160) [IF=6.2]

Application: WB    Species: Mouse    Sample:

Fig. 1. YAP1 knockout aggravates PM2.5-induced lung toxicity and inflammation. WT (YAP1flfl) and YAP1-CKO mice were randomly divided into sham and PM2.5 groups. A and B, The certification of YAP1 conditional knockout in mouse lung tissues; C, Representative YAP1 (red) staining photographs. Nuclei were stained by DAPI (blue); D, Histological images of lung samples exhibited by HE staining in WT mice or YAP1-CKO mice with or without PM2.5 exposure; E, Lung injury scores; F, Representative F4/80 (green) staining photographs, nuclei were stained by DAPI (blue); G, The relative number of F4/80-positive cells was calculated; H, Lung wet/dry mass ratio; I, The protein level of BALF; J and K, Concentrations of TNF-α and IL-6 in lung tissues. The values are shown as the mean ± SD (n = 5),

Application: IF/ICC    Species: Mouse    Sample: lung tissues

Fig. 3. YAP1 knockout accelerates ferroptosis in PM2.5-induced lung toxicity. A, Representative fluorescent images of ROS staining; B, Quantification of ROS labeling intensity in the lung; C, Representative TEM images. The red arrow indicates representative mitochondria in WT or YAP1-CKO mouse lungs exposed to PM2.5 or not; D, Immunoblotting detection of SLC7A11, GPX4 and FTH1 expression in mice with the indicated treatment; quantitative results are shown in E-G n = 5; lung content of Fe2+ (H), MDA (I) and GSH (J) measured by the corresponding commercial kit, n = 5. The values are shown as the mean ± SD,
3). YAP1 alleviates sepsis-induced acute lung injury via inhibiting ferritinophagy-mediated ferroptosis. Frontiers in Immunology, 2022 (PubMed: 35979359) [IF=5.7]
4). Effect of e-cigarette refill liquid on follicular development and estrogen secretion in rats. Tobacco Induced Diseases, 2022 (PubMed: 35529323) [IF=2.2]

Application: WB    Species: Rat    Sample:

Figure 4 Western blot analysis of control, 0.05 mg and 0.5 mg groups of 21- (A) and 35-day-old (B) rat ovaries. Pictures C, D are the relative grey values analysis of CYP19, YAP, PYAP, LATS2, PLATS2 in each group compared with β-actin. Picture C is 21-day-old rat ovaries and D is 35-day-old rat ovaries. Picture E is the estrogen concentration (pg/mL) in each group of 21- and 35-day-old rat ovaries determined by ELISA.

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