AFfirm™ Peroxiredoxin 3 Mouse Monoclonal Antibody - #BF9468

Product: Peroxiredoxin 3 Mouse Monoclonal Antibody
Catalog: BF9468
Description: Mouse monoclonal antibody to Peroxiredoxin 3
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 28kDa; 28kD(Calculated).
Uniprot: P30048

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm9468]
Specificity:
Peroxiredoxin 3 Mouse Monoclonal Antibody detects endogenous levels of total Peroxiredoxin 3.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Antioxidant protein 1; AOP 1; AOP-1; AOP1; HBC189; MER5; MGC104387; MGC24293; mitochondrial; peroxiredoxin 3; Peroxiredoxin III; Peroxiredoxin-3; PRDX3; PRDX3_HUMAN; PRO1748; Protein MER5 homolog; PRX III; Prx-III; PRX3; SP 22; SP-22; SP22; Thioredoxin dependent peroxide reductase mitochondrial; Thioredoxin-dependent peroxide reductase;

Immunogens

Immunogen:
Uniprot:

P30048(PRDX3_HUMAN) >>Visit HPA database.

Gene(ID):
PRDX3(10935)
Description:
This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene.
Sequence:
MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ

PTMs - P30048 As Substrate

Site PTM Type Enzyme
Y71 Phosphorylation
K83 Acetylation
K83 Ubiquitination
S86 Phosphorylation
K91 Acetylation
K91 Ubiquitination
K93 Acetylation
T146 Phosphorylation Q5S007 (LRRK2)
K149 Ubiquitination
Y172 Phosphorylation
S179 Phosphorylation
K196 Acetylation
K196 Sumoylation
K196 Ubiquitination
S199 Phosphorylation
R207 Methylation
R214 Methylation
C229 S-Nitrosylation
T234 Phosphorylation
S237 Phosphorylation
K241 Ubiquitination
K248 Acetylation
K253 Ubiquitination

Research Backgrounds

Function:

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

PTMs:

Phosphorylated by LRRK2; phosphorylation reduces perodixase activity.

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.

Subcellular Location:

Mitochondrion. Cytoplasm. Early endosome.
Note: Localizes to early endosomes in a RPS6KC1-dependent manner.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; disulfide-linked, upon oxidation. 6 homodimers assemble to form a ring-like dodecamer. Interacts with NEK6. Interacts with LRRK2. Interacts with MAP3K13. Interacts with RPS6KC1 (via PX domain).

Family&Domains:

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.

Restrictive clause

 

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