CDK8 Antibody - #DF3186

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Product: CDK8 Antibody
Catalog: DF3186
Description: Rabbit polyclonal antibody to CDK8
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 53 KD; 53kD(Calculated).
Uniprot: P49336
RRID: AB_2835409

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
CDK8 Antibody detects endogenous levels of total CDK8.
RRID:
AB_2835409
Cite Format: Affinity Biosciences Cat# DF3186, RRID:AB_2835409.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cdk8; CDK8 protein kinase; CDK8_HUMAN; Cell Division Kinase 8; Cell division protein kinase 8; Cyclin Dependent kinase 8; Cyclin-dependent kinase 8; K35; Mediator complex subunit cdk8; Mediator of RNA polymerase II transcription subunit cdk8; Protein kinase K35;

Immunogens

Immunogen:
Uniprot:

P49336(CDK8_HUMAN) >>Visit HPA database.

Gene(ID):
CDK8(1024)
Sequence:
MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQIEGTGISMSACREIALLRELKHPNVISLQKVFLSHADRKVWLLFDYAEHDLWHIIKFHRASKANKKPVQLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPYHHDQLDRIFNVMGFPADKDWEDIKKMPEHSTLMKDFRRNTYTNCSLIKYMEKHKVKPDSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLEDPLPTSDVFAGCQIPYPKREFLTEEEPDDKGDKKNQQQQQGNNHTNGTGHPGNQDSSHTQGPPLKKVRVVPPTTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSSMGYSATSQQPPQYSHQTHRY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Bovine
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49336 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation
K26 Ubiquitination
K52 Ubiquitination
K74 Ubiquitination
S80 Phosphorylation
K119 Ubiquitination
S130 Phosphorylation
Y133 Phosphorylation
Y141 Phosphorylation
K272 Ubiquitination
K322 Ubiquitination
T360 Phosphorylation
K402 Acetylation
S413 Phosphorylation

PTMs - P49336 As Enzyme

Substrate Site Source
P24928 (POLR2A) S1619 Uniprot
P42224 (STAT1) S727 Uniprot
P51946 (CCNH) S5 Uniprot
P51946 (CCNH) S304 Uniprot
P84022 (SMAD3) T179 Uniprot
P84022 (SMAD3) S208 Uniprot
P84022 (SMAD3) S213 Uniprot
Q01094 (E2F1) S375 Uniprot
Q15797 (SMAD1) S187 Uniprot
Q15797 (SMAD1) S195 Uniprot
Q15797 (SMAD1) S206 Uniprot
Q15797 (SMAD1) S214 Uniprot

Research Backgrounds

Function:

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. The cylin/CDK pair formed by CCNC/CDK8 also associates with the large subunit of RNA polymerase II. Interacts with CTNNB1, GLI3 and MAML1.

Family&Domains:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

References

1). MiR-382-5p suppresses M1 macrophage polarization and inflammatory response in response to bronchopulmonary dysplasia through targeting CDK8: Involving inhibition of STAT1 pathway. GENES TO CELLS, 2021 (PubMed: 34228857) [IF=1.3]

Application: IF/ICC    Species: Mice    Sample: lung tissues

FIGURE 2 Hyperoxia reduces miR- 382- 5p expression in newborn mice with BPD. (a) By postnatal day 3, newborn mice were exposed to 85% oxygen for establishing the in vivo model of BPD. (b) RT- qPCR assay was performed to detect the expression of miR- 382- 5p in lung tissues of mice with hyperoxia- induced BPD at 5th, 10th and 15th day. (c) Dual immunofluorescence staining of lung tissues with antibodies against CDK8 (red) and CD68 (green). DAPI was used for staining of nuclei (blue). (d) Dual immunofluorescence staining of CD68 (green) and CD86 (red) in the Control and BPD groups. In vivo experiments, n = 6 mice were used in per group and each experiment was repeated at least three times. Data were indicated as mean ± SD. For statistical analysis, data comparison between the Control group and the BPD group was calculated by unpaired Student's t- test. **p < .01 versus the Control group at the same time point

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