Product: SUPT3H Antibody
Catalog: DF3045
Description: Rabbit polyclonal antibody to SUPT3H
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken
Mol.Wt.: 50 KD; 36kD(Calculated).
Uniprot: O75486
RRID: AB_2835428

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(100%), Bovine(88%), Sheep(88%), Dog(88%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
SUPT3H Antibody detects endogenous levels of total SUPT3H.
RRID:
AB_2835428
Cite Format: Affinity Biosciences Cat# DF3045, RRID:AB_2835428.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Positive regulator of Ty transcription; SPT3; SPT3 like protein; Spt3p; SUPT3H; Transcription initiation protein SPT3 homolog; YDR392W;

Immunogens

Immunogen:

A synthesized peptide derived from human SUPT3H, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
O75486 SUPT3_HUMAN:

Expressed in all tissues tested including pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

Sequence:
MNNTAASPMSTATSSSGRSTGKSISFATELQSMMYSLGDARRPLHETAVLVEDVVHTQLINLLQQAAEVSQLRGARVITPEDLLFLMRKDKKKLRRLLKYMFIRDYKSKIVKGIDEDDLLEDKLSGSNNANKRQKIAQDFLNSIDQTGELLAMFEDDEIDEVKQERMERAERQTRIMDSAQYAEFCESRQLSFSKKASKFRDWLDCSSMEIKPNVVAMEILAYLAYETVAQLVDLALLVRQDMVTKAGDPFSHAISATFIQYHNSAESTAACGVEAHSDAIQPCHIREAIRRYSHRIGPLSPFTNAYRRNGMAFLAC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Zebrafish
100
Pig
100
Bovine
88
Sheep
88
Dog
88
Chicken
86
Horse
75
Rabbit
75
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Probable transcriptional activator.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues tested including pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

Family&Domains:

Belongs to the SPT3 family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

Restrictive clause

 

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For Research Use Only.
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