Product: FGR Antibody
Catalog: DF3081
Description: Rabbit polyclonal antibody to FGR
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 55 KD; 59kD(Calculated).
Uniprot: P09769
RRID: AB_2835464

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

FGR Antibody detects endogenous levels of total FGR.
Cite Format: Affinity Biosciences Cat# DF3081, RRID:AB_2835464.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


c fgr; c fgr protooncogene; c src 2 proto oncogene; c src2; Fgr; FGR_HUMAN; FLJ43153; Gardner Rasheed feline sarcoma viral (v fgr); Gardner Rasheed feline sarcoma viral (v fgr) oncogene homolog; Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog; MGC75096; p55 c fgr protein; P55 FGR; P55-FGR; p55c fgr; p58-Fgr; p58c fgr; p58c-Fgr; Proto oncogene c Fgr; Proto oncogene tyrosine protein kinase FGR; Proto-oncogene c-Fgr; SRC 2; SRC2; Tyrosine protein kinase Fgr; Tyrosine-protein kinase Fgr;



Detected in neutrophils, monocytes and natural killer cells (at protein level). Detected in monocytes and large lymphocytes.


PTMs - P09769 As Substrate

Site PTM Type Enzyme
K16 Ubiquitination
Y28 Phosphorylation
Y34 Phosphorylation
Y86 Phosphorylation
S173 Phosphorylation
T176 Phosphorylation
K177 Ubiquitination
Y180 Phosphorylation
S181 Phosphorylation
S183 Phosphorylation
Y208 Phosphorylation
Y209 Phosphorylation
T211 Phosphorylation
T212 Phosphorylation
S261 Phosphorylation
S262 Phosphorylation
T292 Phosphorylation
K301 Ubiquitination
S341 Phosphorylation
Y412 Phosphorylation P09769 (FGR)
T453 Phosphorylation
Y459 Phosphorylation
Y523 Phosphorylation P41240 (CSK) , P09769 (FGR)

PTMs - P09769 As Enzyme

Substrate Site Source
P09769 (FGR) Y412 Uniprot
P09769 (FGR) Y523 Uniprot
P12931-1 (SRC) Y530 Uniprot
P14317 (HCLS1) Y222 Uniprot
P14317 (HCLS1) Y397 Uniprot
P31040 (SDHA) Y543 Uniprot
P31040 (SDHA) Y604 Uniprot
P37840-1 (SNCA) Y125 Uniprot
P68400 (CSNK2A1) Y255 Uniprot
Q05397 (PTK2) Y397 Uniprot
Q05397 (PTK2) Y576 Uniprot
Q05397 (PTK2) Y925 Uniprot
Q99798 (ACO2) Y71 Uniprot
Q99798 (ACO2) Y544 Uniprot
Q99798 (ACO2) Y665 Uniprot

Research Backgrounds


Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1. Together with CLNK, it acts as a negative regulator of natural killer cell-activating receptors and inhibits interferon-gamma production (By similarity).


Ubiquitinated. Becomes ubiquitinated in response to ITGB2 signaling; this does not lead to degradation.

Phosphorylated. Autophosphorylated on tyrosine residues. Becomes phosphorylated in response to FCGR2A and/or FCGR2B engagement, cell adhesion and signaling by ITGB2. Prior phosphorylation at Tyr-523 by SRC inhibits ulterior autophosphorylation at Tyr-412.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Ruffle membrane. Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton. Mitochondrion inner membrane. Mitochondrion intermembrane space.
Note: Detected in mitochondrial intermembrane space and at inner membranes (By similarity). Colocalizes with actin fibers at membrane ruffles. Detected at plasma membrane lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in neutrophils, monocytes and natural killer cells (at protein level). Detected in monocytes and large lymphocytes.

Subunit Structure:

Interacts with ITGB1, ITGB2, MS4A2/FCER1B, FCER1G, FCGR2A and/or FCGR2B. Interacts (via SH2 domain) with SYK (tyrosine phosphorylated). Interacts (via SH2 domain) with FLT3 (tyrosine phosphorylated). Interacts with PTK2/FAK1. Interacts (via SH2 domain) with HCLS1 (tyrosine phosphorylated by SYK). Interacts with SIRPA and PTPNS1. Interacts (not phosphorylated on tyrosine residues) with CBL; FGR tyrosine phosphorylation promotes dissociation. Interacts with PIK3R1 and FASLG (By similarity). Interacts with CLNK (By similarity).


Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Research Fields

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)


1). An Fgr kinase inhibitor attenuates sepsis-associated encephalopathy by ameliorating mitochondrial dysfunction, oxidative stress, and neuroinflammation via the SIRT1/PGC-1α signaling pathway. Journal of Translational Medicine, 2023 (PubMed: 37475042) [IF=7.4]

Application: WB    Species: Mouse    Sample:

Fig. 1 Hippocampal Fgr was increased in the CLP mouse model. A The mRNA levels of Src family kinase members in the hippocampus after CLP surgery (n = 3 for control mice; n = 4 for CLP mice; mean ± SEM, unpaired t-test, *p 

Application: IF/ICC    Species: Mouse    Sample:

Fig. 1 Hippocampal Fgr was increased in the CLP mouse model. A The mRNA levels of Src family kinase members in the hippocampus after CLP surgery (n = 3 for control mice; n = 4 for CLP mice; mean ± SEM, unpaired t-test, *p 

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