HOXB9 Antibody | Affinity Biosciences

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Western blot analysis of extracts from K562 cells, using HOXB9 antibody.

Product:	HOXB9 Antibody
Catalog:	DF3103
Description:	Rabbit polyclonal antibody to HOXB9
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	28 KD; 28kD(Calculated).
Uniprot:	P17482
RRID:	AB_2835480

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(92%), Bovine(92%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(82%)

Clonality:

Polyclonal

Specificity:

HOXB9 Antibody detects endogenous levels of total HOXB9.

RRID:

AB_2835480
Cite Format: Affinity Biosciences Cat# DF3103, RRID:AB_2835480.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Homeo box 2E; Homeo box B9; Homeobox B9; Homeobox protein Hox B9; Homeobox protein Hox-2.5; Homeobox protein Hox-2E; Homeobox protein Hox-B9; Hox 2.5; Hox 2E; HOX2; HOX2E; Hoxb9; HXB9_HUMAN; OTTHUMP00000216946; OTTHUMP00000243325;

Immunogens

Immunogen:

Uniprot:

P17482(HXB9_HUMAN) >>Visit HPA database.

Gene(ID):

HOXB9(3219)

Sequence:

P17482 HXB9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSISGTLSSYYVDSIISHESEDAPPAKFPSGQYASSRQPGHAEHLEFPSCSFQPKAPVFGASWAPLSPHASGSLPSVYHPYIQPQGVPPAESRYLRTWLEPAPRGEAAPGQGQAAVKAEPLLGAPGELLKQGTPEYSLETSAGREAVLSNQRPGYGDNKICEGSEDKERPDQTNPSANWLHARSSRKKRCPYTKYQTLELEKEFLFNMYLTRDRRHEVARLLNLSERQVKIWFQNRRMKMKKMNKEQGKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Dog

Rabbit

Chicken

Horse

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17482 As Substrate

P17482

Site	PTM Type	Source
K27	Acetylation	Uniprot
S30	Phosphorylation	Uniprot
Y33	Phosphorylation	Uniprot
S35	Phosphorylation	Uniprot
S36	Phosphorylation	Uniprot
R93	Methylation	Uniprot
R96	Methylation	Uniprot
K117	Acetylation	Uniprot
T133	Phosphorylation	Uniprot
S137	Phosphorylation	Uniprot
R183	Methylation	Uniprot
Y192	Phosphorylation	Uniprot
K239	Acetylation	Uniprot
K241	Acetylation	Uniprot
K242	Acetylation	Uniprot

Research Backgrounds

P17482_HXB9_HUMAN

Function:

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Subcellular Location:

Nucleus.

Family&Domains:

Belongs to the Abd-B homeobox family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

HOXB9 Antibody - #DF3103