AKAP5 Antibody - #DF3129
| Product: | AKAP5 Antibody |
| Catalog: | DF3129 |
| Description: | Rabbit polyclonal antibody to AKAP5 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Horse, Dog |
| Mol.Wt.: | 47 KD; 47kD(Calculated). |
| Uniprot: | P24588 |
| RRID: | AB_2835506 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3129, RRID:AB_2835506.
Fold/Unfold
A kinase (PRKA) anchor protein 5; A kinase anchor protein 5; A kinase anchor protein 79kDa; A kinase anchoring protein 75/79; A-kinase anchor protein 5; A-kinase anchor protein 79 kDa; AKAP 5; AKAP 75; AKAP 79; AKAP-5; Akap5; AKAP5_HUMAN; AKAP75; AKAP79; cAMP dependent protein kinase regulatory subunit II high affinity binding protein; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein; H21;
Immunogens
Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary.
- P24588 AKAP5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
METTISEIHVENKDEKRSAEGSPGAERQKEKASMLCFKRRKKAAKALKPKAGSEAADVARKCPQEAGASDQPEPTRGAWASLKRLVTRRKRSESSKQQKPLEGEMQPAINAEDADLSKKKAKSRLKIPCIKFPRGPKRSNHSKIIEDSDCSIKVQEEAEILDIQTQTPLNDQATKAKSTQDLSEGISRKDGDEVCESNVSNSTTSGEKVISVELGLDNGHSAIQTGTLILEEIETIKEKQDVQPQQASPLETSETDHQQPVLSDVPPLPAIPDQQIVEEASNSTLESAPNGKDYESTEIVAEETKPKDTELSQESDFKENGITEEKSKSEESKRMEPIAIIITDTEISEFDVTKSKNVPKQFLISAENEQVGVFANDNGFEDRTSEQYETLLIETASSLVKNAIQLSIEQLVNEMASDDNKINNLLQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P24588 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S18 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| T87 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| T179 | Phosphorylation | Uniprot | |
| Y388 | Phosphorylation | Uniprot | |
| T395 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot |
Research Backgrounds
May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.
Palmitoylation at Cys-36 and Cys-129 plays a key role in targeting to lipid rafts.
Membrane>Lipid-anchor.
Note: Associates with lipid rafts.
Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary.
Binding protein for dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (PKA) and also for the protein kinase C (PKC) and the phosphatase calcineurin (PP2B). Each enzyme is inhibited when bound to the anchoring protein. Also binds the beta2-adrenergic receptor. Part of a complex containing AKAP5, ADCY5, ADCY6 AND PDE4C (By similarity). Interacts with ADCY8, and enhances its phosphorylation at lipid rafts.
RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
The N-terminal region, which is highly basic, is required for interaction with calmodulin.
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