Product: AKAP5 Antibody
Catalog: DF3129
Description: Rabbit polyclonal antibody to AKAP5
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Dog
Mol.Wt.: 47 KD; 47kD(Calculated).
Uniprot: P24588
RRID: AB_2835506

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(82%), Horse(80%), Dog(80%)
Clonality:
Polyclonal
Specificity:
AKAP5 Antibody detects endogenous levels of total AKAP5.
RRID:
AB_2835506
Cite Format: Affinity Biosciences Cat# DF3129, RRID:AB_2835506.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A kinase (PRKA) anchor protein 5; A kinase anchor protein 5; A kinase anchor protein 79kDa; A kinase anchoring protein 75/79; A-kinase anchor protein 5; A-kinase anchor protein 79 kDa; AKAP 5; AKAP 75; AKAP 79; AKAP-5; Akap5; AKAP5_HUMAN; AKAP75; AKAP79; cAMP dependent protein kinase regulatory subunit II high affinity binding protein; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein; H21;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P24588 AKAP5_HUMAN:

Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary.

Sequence:
METTISEIHVENKDEKRSAEGSPGAERQKEKASMLCFKRRKKAAKALKPKAGSEAADVARKCPQEAGASDQPEPTRGAWASLKRLVTRRKRSESSKQQKPLEGEMQPAINAEDADLSKKKAKSRLKIPCIKFPRGPKRSNHSKIIEDSDCSIKVQEEAEILDIQTQTPLNDQATKAKSTQDLSEGISRKDGDEVCESNVSNSTTSGEKVISVELGLDNGHSAIQTGTLILEEIETIKEKQDVQPQQASPLETSETDHQQPVLSDVPPLPAIPDQQIVEEASNSTLESAPNGKDYESTEIVAEETKPKDTELSQESDFKENGITEEKSKSEESKRMEPIAIIITDTEISEFDVTKSKNVPKQFLISAENEQVGVFANDNGFEDRTSEQYETLLIETASSLVKNAIQLSIEQLVNEMASDDNKINNLLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
82
Horse
80
Dog
80
Rabbit
73
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24588 As Substrate

Site PTM Type Enzyme
S18 Phosphorylation
S22 Phosphorylation
S53 Phosphorylation
S69 Phosphorylation
S81 Phosphorylation
T87 Phosphorylation
S92 Phosphorylation
S95 Phosphorylation
S148 Phosphorylation
S151 Phosphorylation
S178 Phosphorylation
T179 Phosphorylation
Y388 Phosphorylation
T395 Phosphorylation
S398 Phosphorylation

Research Backgrounds

Function:

May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.

PTMs:

Palmitoylation at Cys-36 and Cys-129 plays a key role in targeting to lipid rafts.

Subcellular Location:

Membrane>Lipid-anchor.
Note: Associates with lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary.

Subunit Structure:

Binding protein for dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (PKA) and also for the protein kinase C (PKC) and the phosphatase calcineurin (PP2B). Each enzyme is inhibited when bound to the anchoring protein. Also binds the beta2-adrenergic receptor. Part of a complex containing AKAP5, ADCY5, ADCY6 AND PDE4C (By similarity). Interacts with ADCY8, and enhances its phosphorylation at lipid rafts.

Family&Domains:

RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

The N-terminal region, which is highly basic, is required for interaction with calmodulin.

Restrictive clause

 

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