HAMP Recombinant Rabbit mAb - #BF3309

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Product: HAMP Recombinant Rabbit mAb
Catalog: BF3309
Description: Rabbit monoclonal antibody to HAMP
Application: WB
Reactivity: Human, Rat
Mol.Wt.: 9,3 kDa(Observed); 9kD(Calculated).
Uniprot: P81172

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Data Sheet
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Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Monoclonal [ReFirm22756]
Specificity:
HAMP Recombinant Rabbit mAb detects endogenous levels of HAMP.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hamp; HEPC; HEPC_HUMAN; Hepc20; Hepc25; Hepcidin-20; HFE2B; LEAP-1; LEAP1; Liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor;

Immunogens

Immunogen:

A synthetic peptide from human HAMP

Uniprot:

P81172(HEPC_HUMAN) >>Visit HPA database.

Gene(ID):
HAMP(57817)
Expression:
P81172 HEPC_HUMAN:

Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.

Description:
The product encoded by this gene is involved in the maintenance of iron homeostasis,and it is necessary for the regulation of iron storage in macrophages,and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20,22 and 25 amino acids,and these active peptides are rich in cysteines,which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity against bacteria and fungi. Mutations in this gene cause hemochromatosis type 2B,also known as juvenile hemochromatosis,a disease caused by severe iron overload that results in cardiomyopathy,cirrhosis,and endocrine failure.
Sequence:
MALSSQIWAACLLLLLLLASLTSGSVFPQQTGQLAELQPQDRAGARASWMPMFQRRRRRDTHFPICIFCCGCCHRSKCGMCCKT

Research Backgrounds

Function:

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.

Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.

Family&Domains:

Belongs to the hepcidin family.

Restrictive clause

 

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For Research Use Only.
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