Product: HMGB2 Antibody
Catalog: DF3133
Description: Rabbit polyclonal antibody to HMGB2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 27 KD; 24kD(Calculated).
Uniprot: P26583
RRID: AB_2835510

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%)
HMGB2 Antibody detects endogenous levels of total HMGB2.
Cite Format: Affinity Biosciences Cat# DF3133, RRID:AB_2835510.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


C80539; High mobility group (nonhistone chromosomal) protein 2; High mobility group box 2; High mobility group protein 2; High mobility group protein B2; HMG 2; HMG B2; HMG-2; HMG2; HMGB2; HMGB2_HUMAN;



Expressed in gastric and intestinal tissues (at protein level).




Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P26583 As Substrate

Site PTM Type Enzyme
K3 Acetylation
R10 Methylation
K12 Acetylation
K12 Ubiquitination
S15 Phosphorylation
Y16 Phosphorylation
R24 Methylation
K30 Acetylation
K30 Ubiquitination
S35 Phosphorylation
S42 Phosphorylation
K43 Acetylation
K43 Ubiquitination
K44 Acetylation
K44 Ubiquitination
K50 Ubiquitination
T51 Phosphorylation
S53 Phosphorylation
K55 Acetylation
K55 Ubiquitination
K59 Acetylation
K59 Ubiquitination
S66 Phosphorylation
K76 Ubiquitination
Y78 Phosphorylation
K85 Acetylation
S100 Phosphorylation
S107 Phosphorylation
R110 Methylation
K112 Methylation
K114 Ubiquitination
S115 Phosphorylation
S121 Phosphorylation
K127 Ubiquitination
K128 Ubiquitination
S134 Phosphorylation
S137 Phosphorylation
K139 Acetylation
K139 Ubiquitination
K141 Ubiquitination
K147 Acetylation
K147 Ubiquitination
K150 Ubiquitination
K154 Acetylation
Y155 Phosphorylation
K157 Acetylation
K157 Ubiquitination
Y162 Phosphorylation
S168 Phosphorylation
K172 Acetylation
K173 Acetylation
T179 Phosphorylation
S181 Phosphorylation
K182 Acetylation
K183 Acetylation

Research Backgrounds


Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V(D)J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity. Involved in V(D)J recombination by acting as a cofactor of the RAG complex: acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a promiscuous immunogenic DNA/RNA sensor which cooperates with subsequent discriminative sensing by specific pattern recognition receptors (By similarity). In the extracellular compartment acts as a chemokine. Promotes proliferation and migration of endothelial cells implicating AGER/RAGE. Has antimicrobial activity in gastrointestinal epithelial tissues. Involved in inflammatory response to antigenic stimulus coupled with proinflammatory activity (By similarity). Involved in modulation of neurogenesis probably by regulation of neural stem proliferation (By similarity). Involved in articular cartilage surface maintenance implicating LEF1 and the Wnt/beta-catenin pathway (By similarity).


Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB2 (HMGB2C23hC45hC106h), 2- disulfide HMGB2 (HMGB2C23-C45C106h) and 3- sulfonyl HMGB2 (HMGB2C23soC45soC106so).

Acetylation enhances nucleosome binding and chromation remodeling activity.

Subcellular Location:

Nucleus. Chromosome. Cytoplasm. Secreted.
Note: In basal state predominantly nuclear.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in gastric and intestinal tissues (at protein level).

Subunit Structure:

Interacts with POU2F2, POU2F1 and POU3F1 (By similarity). Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2 (By similarity). Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Directly interacts with SET. Interacts with LEF1 (By similarity).


Both, HMG box 1 and HMG box 2, show antimicrobial activity.

Belongs to the HMGB family.


1). HMGB2 promotes chondrocyte proliferation under negative pressure through the phosphorylation of AKT. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2021 (PubMed: 34333060) [IF=5.1]

Application: IHC    Species: Mice    Sample: cartilage tissue

Fig. 2. Negative pressure increased HMGB2 expression and AKT phosphorylation together with chondrocyte proliferation. (A) Heatmap of representative molecules revealed by RNA-seq (n = 3). (B) Representative images of EdU staining of CON and NP groups. (C) qRT-PCR analysis of Akt, P21, Hmgb2, Ki67 and CDK6 expression (n = 5). (D) Western blotting analysis of the expression of related molecules (n = 3). (E) qRT-PCR analysis of Akt, P21, Hmgb2, Ki67 and CDK6 expression in the TMJ condylar cartilage of mice in Sham and BAE groups (n = 6). (F) Representative staining of Ki67, HMGB2 and P-AKT of TMJ condylar cartilage of mice in Sham and BAE groups (n = 6). All data are presented as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.

Application: WB    Species: Mice    Sample: cartilage tissue

Fig. 3. Interference with AKT phosphorylation affected the proliferation-promoting effect of nega- tive pressure on superficial cells derived from TMJ condyles of 3-week-old rats. (A) Western blotting analysis (n = 3) of AKT phosphorylation changes after treatment with LY294002. (B) qRT-PCR analysis (n = 5) and (C–D) western blotting analysis (n = 3) of Ki67, CDK6 and HMGB2 for the treatment of LY294002 in CON and NP groups. (E) Western blot- ting analysis (n = 3) of AKT phosphorylation changes after treatment with LY294002. qRT-PCR analysis (n = 6) (F) and western blotting analysis (n = 3) (G–H) of Ki67, CDK6 and HMGB2 for the treatment of LY294002 in CON and NP groups. All data are pre- sented as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.

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