MARK3 Antibody - #DF3135
Product: | MARK3 Antibody |
Catalog: | DF3135 |
Description: | Rabbit polyclonal antibody to MARK3 |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Pig, Rabbit, Dog, Chicken |
Mol.Wt.: | 85 KD; 84kD(Calculated). |
Uniprot: | P27448 |
RRID: | AB_2835512 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3135, RRID:AB_2835512.
Fold/Unfold
C-TAK1; Cdc25C associated protein kinase 1; Cdc25C-associated protein kinase 1; cTAK1; ELKL motif kinase 2; EMK-2; Emk2; ETK 1; KIAA4230; KP78; MAP microtubule affinity regulating kinase 3; MAP/microtubule affinity-regulating kinase 3; Mark3; MARK3_HUMAN; Par 1a; PAR1A; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Serine threonine protein kinase p78; Serine/threonine-protein kinase p78; SerThr protein kinase PAR 1;
Immunogens
- P27448 MARK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTRTPLPTVNERDTENHTSHGDGRQEVTSRTSRSGARCRNSIASCADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGGKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLEQIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLSKMKYDEITATYLLLGRKSSELDASDSSSSSNLSLAKVRPSSDLNNSTGQSPHHKVQRSVSSSQKQRRYSDHAGPAIPSVVAYPKRSQTSTADSDLKEDGISSRKSSGSAVGGKGIAPASPMLGNASNPNKADIPERKKSSTVPSSNTASGGMTRRNTYVCSERTTADRHSVIQNGKENSTIPDQRTPVASTHSISSAATPDRIRFPRGTASRSTFHGQPRERRTATYNGPPASPSLSHEATPLSQTRSRGSTNLFSKLTSKLTRRNMSFRFIKRLPTEYERNGRYEGSSRNVSAEQKDENKEAKPRSLRFTWSMKTTSSMDPGDMMREIRKVLDANNCDYEQRERFLLFCVHGDGHAENLVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIASKIANELKL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P27448 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
T3 | Phosphorylation | Uniprot | |
T5 | Phosphorylation | Uniprot | |
S42 | Phosphorylation | Uniprot | |
S45 | Phosphorylation | Uniprot | |
T61 | Phosphorylation | Uniprot | |
K64 | Ubiquitination | Uniprot | |
K85 | Ubiquitination | Uniprot | |
K89 | Ubiquitination | Uniprot | |
T90 | Phosphorylation | P11309 (PIM1) | Uniprot |
T95 | Phosphorylation | P11309 (PIM1) | Uniprot |
S96 | Phosphorylation | P11309 (PIM1) | Uniprot |
K180 | Ubiquitination | Uniprot | |
T211 | Phosphorylation | Q15831 (STK11) | Uniprot |
S215 | Phosphorylation | Q15831 (STK11) | Uniprot |
K227 | Ubiquitination | Uniprot | |
S238 | Phosphorylation | Uniprot | |
K294 | Ubiquitination | Uniprot | |
K303 | Ubiquitination | Uniprot | |
K317 | Ubiquitination | Uniprot | |
K330 | Ubiquitination | Uniprot | |
Y340 | Phosphorylation | Uniprot | |
S341 | Phosphorylation | Uniprot | |
K367 | Ubiquitination | Uniprot | |
S374 | Phosphorylation | Uniprot | |
S376 | Phosphorylation | Uniprot | |
S377 | Phosphorylation | Uniprot | |
S378 | Phosphorylation | Uniprot | |
S379 | Phosphorylation | Uniprot | |
S380 | Phosphorylation | Uniprot | |
S383 | Phosphorylation | Uniprot | |
S396 | Phosphorylation | Uniprot | |
T397 | Phosphorylation | Uniprot | |
S400 | Phosphorylation | Uniprot | |
Y418 | Phosphorylation | Uniprot | |
S419 | Phosphorylation | Uniprot | |
Y432 | Phosphorylation | Uniprot | |
T438 | Phosphorylation | Uniprot | |
S455 | Phosphorylation | Uniprot | |
S456 | Phosphorylation | Uniprot | |
S458 | Phosphorylation | Uniprot | |
S469 | Phosphorylation | Uniprot | |
S476 | Phosphorylation | Uniprot | |
S489 | Phosphorylation | Uniprot | |
S490 | Phosphorylation | Uniprot | |
T491 | Phosphorylation | Uniprot | |
S494 | Phosphorylation | Uniprot | |
S499 | Phosphorylation | Uniprot | |
T503 | Phosphorylation | Uniprot | |
T507 | Phosphorylation | P17612 (PRKACA) | Uniprot |
Y508 | Phosphorylation | Uniprot | |
S511 | Phosphorylation | Uniprot | |
S520 | Phosphorylation | Uniprot | |
T530 | Phosphorylation | Uniprot | |
T536 | Phosphorylation | Uniprot | |
S540 | Phosphorylation | Uniprot | |
T541 | Phosphorylation | Uniprot | |
S543 | Phosphorylation | Uniprot | |
S545 | Phosphorylation | Uniprot | |
S546 | Phosphorylation | Uniprot | |
T549 | Phosphorylation | Uniprot | |
S563 | Phosphorylation | Uniprot | |
T564 | Phosphorylation | P41743 (PRKCI) , Q05513 (PRKCZ) | Uniprot |
T574 | Phosphorylation | Uniprot | |
T576 | Phosphorylation | Uniprot | |
Y577 | Phosphorylation | Uniprot | |
S583 | Phosphorylation | Uniprot | |
S585 | Phosphorylation | Uniprot | |
S587 | Phosphorylation | Uniprot | |
T591 | Phosphorylation | Uniprot | |
S594 | Phosphorylation | Uniprot | |
T596 | Phosphorylation | Uniprot | |
S598 | Phosphorylation | Uniprot | |
R599 | Methylation | Uniprot | |
S601 | Phosphorylation | Uniprot | |
S606 | Phosphorylation | Uniprot | |
S610 | Phosphorylation | Uniprot | |
K611 | Ubiquitination | Uniprot | |
S618 | Phosphorylation | Uniprot | |
S643 | Phosphorylation | Uniprot | |
S657 | Phosphorylation | Uniprot | |
K681 | Ubiquitination | Uniprot | |
Y690 | Phosphorylation | Uniprot | |
S724 | Phosphorylation | Uniprot | |
K741 | Ubiquitination | Uniprot | |
S745 | Phosphorylation | Uniprot |
PTMs - P27448 As Enzyme
Research Backgrounds
Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1.
Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-564 by PRKCZ/aPKC inhibits the kinase activity.
Cell membrane>Peripheral membrane protein. Cell projection>Dendrite. Cytoplasm.
Ubiquitous.
Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.