TEAD1 Antibody - #DF3141

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Product: TEAD1 Antibody
Catalog: DF3141
Description: Rabbit polyclonal antibody to TEAD1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 48 KD; 48kD(Calculated).
Uniprot: P28347
RRID: AB_2835518

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TEAD1 Antibody detects endogenous levels of total TEAD1.
RRID:
AB_2835518
Cite Format: Affinity Biosciences Cat# DF3141, RRID:AB_2835518.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl,sodium azide and glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DTEF 1; NTEF 1; Protein GT-IIC; SV40 transcriptional enhancer factor; TCF 13; TCF13; TEA domain family member 1; TEA domain family member 2; TEA domain family member 3; TEA domain family member 4; TEAD 1; TEAD 2; TEAD 3; TEAD 4; TEAD2; TEAD3; TEAD4; TEAD5; TEF1; TEF4; TEF5; Transcription factor 13; Transcription factor 13 like 1; Transcription factor RTEF 1; Transcriptional enhancer factor TEF 1; Transcriptional enhancer factor TEF 3; Transcriptional enhancer factor TEF 4; Transcriptional enhancer factor TEF 5;

Immunogens

Immunogen:
Uniprot:

P28347(TEAD1_HUMAN) >>Visit HPA database.

Gene(ID):
TEAD1(7003)
Expression:
P28347 TEAD1_HUMAN:

Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.

Sequence:
MEPSSWSGSESPAENMERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKSRDFHSKLKDQTAKDKALQHMAAMSSAQIVSATAIHNKLGLPGIPRPTFPGAPGFWPGMIQTGQPGSSQDVKPFVQQAYPIQPAVTAPIPGFEPASAPAPSVPAWQGRSIGTTKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVTSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRLVKD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Pig
55
Chicken
55
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P28347 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S4 Phosphorylation
S7 Phosphorylation
S9 Phosphorylation
S11 Phosphorylation
S20 Phosphorylation
S36 Phosphorylation
K57 Ubiquitination
S61 Phosphorylation
K65 Ubiquitination
Y76 Phosphorylation
K83 Methylation
K88 Ubiquitination
K101 Methylation
S102 Phosphorylation
K108 Ubiquitination
K117 Ubiquitination
K173 Sumoylation
Y263 Phosphorylation
K265 Ubiquitination
K270 Acetylation
K274 Acetylation
K331 Ubiquitination

Research Backgrounds

Function:

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.

Subunit Structure:

Interacts with YAP1 and WWTR1/TAZ.

Research Fields

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

References

1). Expression of Key Factors of the Hippo Signaling Pathway in Yak (Bos grunniens) Mammary Gland. Animals [IF=3.0]

Application: WB    Species: Mouse    Sample:

Figure 3. Immunoblotting bands (a) and statistical analysis (b) of MST1, LATS1, YAP1, TEAD1, and β-actin for: 1, GP; 2, LP; 3, DP. The relative abundance of target proteins at each stage was expressed as the ratio of the measured intensity of MST1, LATS1, YAP1, TEAD1 protein, and β-Actin protein (target protein/β-actin). **, the expression of the same protein was significantly different at different periods

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