Involucrin Recombinant Rabbit mAb - #BF3420

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Product: Involucrin Recombinant Rabbit mAb
Catalog: BF3420
Description: Rabbit monoclonal antibody to Involucrin
Application: WB IHC
Reactivity: Human, Rat
Mol.Wt.: 70,120-140kD(Observed); 68kD(Calculated).
Uniprot: P07476

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 100ul $280 In stock

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IHC Protocol
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Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:3000, IHC 1:100
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Rat
Clonality:
Monoclonal [ReFirm22867]
Specificity:
Involucrin Recombinant Rabbit mAb detects endogenous levels of Involucrin.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

INVO_HUMAN; Involucrin; IVL;

Immunogens

Immunogen:

A synthetic peptide from human Involucrin

Uniprot:

P07476(INVO_HUMAN) >>Visit HPA database.

Gene(ID):
IVL(3713)
Expression:
P07476 INVO_HUMAN:

Keratinocytes of epidermis and other stratified squamous epithelia.

Description:
Involucrin,a component of the keratinocyte crosslinked envelope,is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21,among calpactin I light chain,trichohyalin,profillaggrin,loricrin,and calcyclin.
Sequence:
MSQQHTLPVTLSPALSQELLKTVPPPVNTHQEQMKQPTPLPPPCQKVPVELPVEVPSKQEEKHMTAVKGLPEQECEQQQKEPQEQELQQQHWEQHEEYQKAENPEQQLKQEKTQRDQQLNKQLEEEKKLLDQQLDQELVKRDEQLGMKKEQLLELPEQQEGHLKHLEQQEGQLKHPEQQEGQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPQQQEGQLELSEQQEGQLELSEQQEGQLKHLEHQEGQLEVPEEQMGQLKYLEQQEGQLKHLDQQEKQPELPEQQMGQLKHLEQQEGQPKHLEQQEGQLEQLEEQEGQLKHLEQQEGQLEHLEHQEGQLGLPEQQVLQLKQLEKQQGQPKHLEEEEGQLKHLVQQEGQLKHLVQQEGQLEQQERQVEHLEQQVGQLKHLEEQEGQLKHLEQQQGQLEVPEQQVGQPKNLEQEEKQLELPEQQEGQVKHLEKQEAQLELPEQQVGQPKHLEQQEKHLEHPEQQDGQLKHLEQQEGQLKDLEQQKGQLEQPVFAPAPGQVQDIQPALPTKGEVLLPVEHQQQKQEVQWPPKHK

Research Backgrounds

Function:

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

PTMs:

Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

Subcellular Location:

Cytoplasm.
Note: Constituent of the scaffolding of the cornified envelope.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Keratinocytes of epidermis and other stratified squamous epithelia.

Family&Domains:

Belongs to the involucrin family.

Restrictive clause

 

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