Product: MZF-1 Antibody
Catalog: DF3142
Description: Rabbit polyclonal antibody to MZF-1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Dog
Mol.Wt.: 82 KD; 82kD(Calculated).
Uniprot: P28698
RRID: AB_2835519

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Horse(90%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MZF-1 Antibody detects endogenous levels of total MZF-1.
RRID:
AB_2835519
Cite Format: Affinity Biosciences Cat# DF3142, RRID:AB_2835519.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Myeloid zinc finger 1; MZF 1; MZF-1; MZF1; MZF1_HUMAN; MZF1B; Zfp98; Zinc finger and SCAN domain containing protein 6; Zinc finger and SCAN domain-containing protein 6; Zinc finger protein 42; ZNF42; ZSCAN6;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P28698 MZF1_HUMAN:

Preferentially expressed in differentiating myeloid cells. Detected in osteoblasts.

Sequence:
MRPAVLGSPDRAPPEDEGPVMVKLEDSEEEGEAALWDPGPEAARLRFRCFRYEEATGPQEALAQLRELCRQWLRPEVRSKEQMLELLVLEQFLGALPPEIQARVQGQRPGSPEEAAALVDGLRREPGGPRRWVTVQVQGQEVLSEKMEPSSFQPLPETEPPTPEPGPKTPPRTMQESPLGLQVKEESEVTEDSDFLESGPLAATQESVPTLLPEEAQRCGTVLDQIFPHSKTGPEGPSWREHPRALWHEEAGGIFSPGFALQLGSISAGPGSVSPHLHVPWDLGMAGLSGQIQSPSREGGFAHALLLPSDLRSEQDPTDEDPCRGVGPALITTRWRSPRGRSRGRPSTGGGVVRGGRCDVCGKVFSQRSNLLRHQKIHTGERPFVCSECGRSFSRSSHLLRHQLTHTEERPFVCGDCGQGFVRSARLEEHRRVHTGEQPFRCAECGQSFRQRSNLLQHQRIHGDPPGPGAKPPAPPGAPEPPGPFPCSECRESFARRAVLLEHQAVHTGDKSFGCVECGERFGRRSVLLQHRRVHSGERPFACAECGQSFRQRSNLTQHRRIHTGERPFACAECGKAFRQRPTLTQHLRVHTGEKPFACPECGQRFSQRLKLTRHQRTHTGEKPYHCGECGLGFTQVSRLTEHQRIHTGERPFACPECGQSFRQHANLTQHRRIHTGERPYACPECGKAFRQRPTLTQHLRTHRREKPFACQDCGRRFHQSTKLIQHQRVHSAE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
90
Horse
90
Bovine
73
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P28698 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation
S27 Phosphorylation
S111 Phosphorylation
T134 Phosphorylation
T169 Phosphorylation
S177 Phosphorylation
S256 Phosphorylation Q16539 (MAPK14) , P27361 (MAPK3)
S274 Phosphorylation Q16539 (MAPK14) , P27361 (MAPK3)
S294 Phosphorylation Q16539 (MAPK14) , P27361 (MAPK3)
S347 Phosphorylation
T592 Phosphorylation
K595 Sumoylation
T618 Phosphorylation
T620 Phosphorylation
K723 Sumoylation

Research Backgrounds

Function:

Binds to target promoter DNA and functions as transcription regulator. Regulates transcription from the PADI1 and CDH2 promoter. May be one regulator of transcriptional events during hemopoietic development.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Preferentially expressed in differentiating myeloid cells. Detected in osteoblasts.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the krueppel C2H2-type zinc-finger protein family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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