ERCC5 Antibody - #DF3143

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Product: ERCC5 Antibody
Catalog: DF3143
Description: Rabbit polyclonal antibody to ERCC5
Application: WB IF/ICC
Reactivity: Human
Prediction: Bovine, Dog
Mol.Wt.: 130 KD; 133kD(Calculated).
Uniprot: P28715
RRID: AB_2835520

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(80%), Dog(80%)
Clonality:
Polyclonal
Specificity:
ERCC5 Antibody detects endogenous levels of total ERCC5.
RRID:
AB_2835520
Cite Format: Affinity Biosciences Cat# DF3143, RRID:AB_2835520.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

COFS 3; COFS3; DNA excision repair protein ERCC 5; DNA excision repair protein ERCC-5; DNA excision repair protein ERCC5; DNA repair protein complementing XP G cells; DNA repair protein complementing XP-G cells; DNA repair protein complementing XPG cells; ERCC 5; ERCC5; ERCC5_HUMAN; ERCM 2; ERCM2; Excision repair cross complementation group 5; Excision Repair Cross Complementing Rodent Repair Deficiency; Excision repair cross complementing rodent repair deficiency complementation group 5; Excision repair protein; OTTHUMP00000064902; UVDR; Xeroderma Pigmentosum Complementation Group G; Xeroderma pigmentosum complementation group G protein; Xeroderma pigmentosum group G complementing protein; Xeroderma pigmentosum group G-complementing protein; XPG; XPG complementing protein; XPGC;

Immunogens

Immunogen:
Uniprot:

P28715(ERCC5_HUMAN) >>Visit HPA database.

Gene(ID):
ERCC5(2073)
Sequence:
MGVQGLWKLLECSGRQVSPEALEGKILAVDISIWLNQALKGVRDRHGNSIENPHLLTLFHRLCKLLFFRIRPIFVFDGDAPLLKKQTLVKRRQRKDLASSDSRKTTEKLLKTFLKRQAIKTAFRSKRDEALPSLTQVRRENDLYVLPPLQEEEKHSSEEEDEKEWQERMNQKQALQEEFFHNPQAIDIESEDFSSLPPEVKHEILTDMKEFTKRRRTLFEAMPEESDDFSQYQLKGLLKKNYLNQHIEHVQKEMNQQHSGHIRRQYEDEGGFLKEVESRRVVSEDTSHYILIKGIQAKTVAEVDSESLPSSSKMHGMSFDVKSSPCEKLKTEKEPDATPPSPRTLLAMQAALLGSSSEEELESENRRQARGRNAPAAVDEGSISPRTLSAIKRALDDDEDVKVCAGDDVQTGGPGAEEMRINSSTENSDEGLKVRDGKGIPFTATLASSSVNSAEEHVASTNEGREPTDSVPKEQMSLVHVGTEAFPISDESMIKDRKDRLPLESAVVRHSDAPGLPNGRELTPASPTCTNSVSKNETHAEVLEQQNELCPYESKFDSSLLSSDDETKCKPNSASEVIGPVSLQETSSIVSVPSEAVDNVENVVSFNAKEHENFLETIQEQQTTESAGQDLISIPKAVEPMEIDSEESESDGSFIEVQSVISDEELQAEFPETSKPPSEQGEEELVGTREGEAPAESESLLRDNSERDDVDGEPQEAEKDAEDSLHEWQDINLEELETLESNLLAQQNSLKAQKQQQERIAATVTGQMFLESQELLRLFGIPYIQAPMEAEAQCAILDLTDQTSGTITDDSDIWLFGARHVYRNFFNKNKFVEYYQYVDFHNQLGLDRNKLINLAYLLGSDYTEGIPTVGCVTAMEILNEFPGHGLEPLLKFSEWWHEAQKNPKIRPNPHDTKVKKKLRTLQLTPGFPNPAVAEAYLKPVVDDSKGSFLWGKPDLDKIREFCQRYFGWNRTKTDESLFPVLKQLDAQQTQLRIDSFFRLAQQEKEDAKRIKSQRLNRAVTCMLRKEKEAAASEIEAVSVAMEKEFELLDKAKGKTQKRGITNTLEESSSLKRKRLSDSKGKNTCGGFLGETCLSESSDGSSSEDAESSSLMNVQRRTAAKEPKTSASDSQNSVKEAPVKNGGATTSSSSDSDDDGGKEKMVLVTARSVFGKKRRKLRRARGRKRKT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
80
Dog
80
Pig
0
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P28715 As Substrate

Site PTM Type Enzyme
K8 Acetylation
K8 Ubiquitination
K64 Ubiquitination
S100 Phosphorylation
K111 Ubiquitination
K120 Ubiquitination
K126 Ubiquitination
S156 Phosphorylation
S157 Phosphorylation
K172 Ubiquitination
K209 Ubiquitination
T217 Phosphorylation
K235 Ubiquitination
K240 Ubiquitination
K252 Ubiquitination
K274 Ubiquitination
Y289 Phosphorylation
K293 Ubiquitination
K298 Ubiquitination
S307 Phosphorylation
K313 Ubiquitination
S318 Phosphorylation
K322 Ubiquitination
T331 Phosphorylation
T338 Phosphorylation
S341 Phosphorylation
T344 Phosphorylation
S355 Phosphorylation
S356 Phosphorylation
S357 Phosphorylation
S363 Phosphorylation
S382 Phosphorylation
S384 Phosphorylation
S389 Phosphorylation
K392 Ubiquitination
K402 Ubiquitination
S423 Phosphorylation
S424 Phosphorylation
S428 Phosphorylation
K433 Ubiquitination
K438 Acetylation
S477 Phosphorylation
T523 Phosphorylation
S526 Phosphorylation
S558 Phosphorylation
S559 Phosphorylation
S562 Phosphorylation
S563 Phosphorylation
T567 Phosphorylation
S573 Phosphorylation
S662 Phosphorylation
S678 Phosphorylation
S699 Phosphorylation
S705 Phosphorylation
S724 Phosphorylation
T738 Phosphorylation
K751 Ubiquitination
K904 Ubiquitination
T920 Phosphorylation
T924 Phosphorylation
K952 Ubiquitination
K972 Ubiquitination
K982 Ubiquitination
S1012 Phosphorylation
T1020 Phosphorylation
S1032 Phosphorylation
K1050 Ubiquitination
S1069 Phosphorylation
K1071 Methylation
K1071 Ubiquitination
S1129 Phosphorylation
S1132 Phosphorylation
K1139 Acetylation
T1145 Phosphorylation
S1147 Phosphorylation
S1148 Phosphorylation
S1149 Phosphorylation
S1151 Phosphorylation

Research Backgrounds

Function:

Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with PCNA.

Family&Domains:

Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.

Research Fields

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

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