TBX1 Antibody | Affinity Biosciences

WB
Cites

1/2

Western blot analysis on COLO205 cell lysates using TBX1 Antibody.

Product:	TBX1 Antibody
Catalog:	AF0327
Description:	Rabbit polyclonal antibody to TBX1
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	43kDa; 43kD(Calculated).
Uniprot:	O43435
RRID:	AB_2833490

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

TBX1 Antibody detects endogenous levels of total TBX1.

RRID:

AB_2833490
Cite Format: Affinity Biosciences Cat# AF0327, RRID:AB_2833490.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Brachyury; CAFS; CTHM; DGCR; DGS; DORV; T box; T box 1; T box 1 transcription factor; T box 1 transcription factor C; T box protein 1; T box transcription factor TBX 1; T box transcription factor TBX1; T-box protein 1; T-box transcription factor TBX1; TBX 1; TBX 1C; tbx1; TBX1_HUMAN; TBX1C; Testis specific T box protein; Testis-specific T-box protein; TGA; VCFS;

Immunogens

Immunogen:

A synthesized peptide derived from human TBX1, corresponding to a region within C-terminal amino acids.

Uniprot:

O43435(TBX1_HUMAN) >>Visit HPA database.

Gene(ID):

TBX1(6899)

Description:

TBX1 Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries. 3 isoforms of the human protein are produced by alternative splicing.

Sequence:

O43435 TBX1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MHFSTVTRDMEAFTASSLSSLGAAGGFPGAASPGADPYGPREPPPPPPRYDPCAAAAPGAPGPPPPPHAYPFAPAAGAATSAAAEPEGPGASCAAAAKAPVKKNAKVAGVSVQLEMKALWDEFNQLGTEMIVTKAGRRMFPTFQVKLFGMDPMADYMLLMDFVPVDDKRYRYAFHSSSWLVAGKADPATPGRVHYHPDSPAKGAQWMKQIVSFDKLKLTNNLLDDNGHIILNSMHRYQPRFHVVYVDPRKDSEKYAEENFKTFVFEETRFTAVTAYQNHRITQLKIASNPFAKGFRDCDPEDWPRNHRPGALPLMSAFARSRNPVASPTQPSGTEKGGHVLKDKEVKAETSRNTPEREVELLRDAGGCVNLGLPCPAECQPFNTQGLVAGRTAGDRLC

PTMs - O43435 As Substrate

O43435

Site	PTM Type	Source
T142	Phosphorylation	Uniprot
K202	Ubiquitination	Uniprot
Y255	Phosphorylation	Uniprot
T282	Phosphorylation	Uniprot
S327	Phosphorylation	Uniprot

Research Backgrounds

O43435_TBX1_HUMAN

Function:

Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries (By similarity).

Subcellular Location:

Nucleus.

Subunit Structure:

Interacts with DSCR6.

References

1). Establishment of an induced pluripotent stem cell line (SAHGMUi001-A) from a patient with Netherton syndrome carrying SPINK5 mutation. Stem Cell Research, 2021 (PubMed: 32416580) [IF=1.2]

2). Generation and characterization of an induced pluripotent stem cell line (ZSYYDNi001-A) from a patient with Duchenne muscular dystrophy carrying exon 51 deletion in the DMD gene. Stem Cell Research, 2021 (PubMed: 34619646) [IF=1.2]

Application: IF/ICC Species: Human Sample:

Fig. 1. Characterization of the induced pluripotent stem cell line.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TBX1 Antibody - #AF0327