MMP8 Recombinant Rabbit mAb - #BF3747

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Product: MMP8 Recombinant Rabbit mAb
Catalog: BF3747
Description: Rabbit monoclonal antibody to MMP8
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 70 kDa(Observed); 53kD(Calculated).
Uniprot: P22894

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 100ul $280 In stock

Lead Time: Same day delivery

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Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:5000, IHC 1:100-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse, Rat
Clonality:
Monoclonal [ReFirm23194]
Specificity:
MMP8 Recombinant Rabbit mAb detects endogenous levels of MMP8.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CLG 1; CLG1; Collagenase 1; Collagenase 1 neutrophil; HNC; Matrix metallopeptidase 8 (neutrophil collagenase); Matrix metalloprotease 8; Matrix metalloproteinase-8; MMP 8; MMP-8; Mmp8; MMP8_HUMAN; Neutrophil collagenase; PMNL CL; PMNL collagenase; PMNL-CL; PMNLCL;

Immunogens

Immunogen:

A synthetic peptide from human MMP8

Uniprot:

P22894(MMP8_HUMAN) >>Visit HPA database.

Gene(ID):
MMP8(4317)
Expression:
P22894 MMP8_HUMAN:

Neutrophils.

Description:
This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development,reproduction,and tissue remodeling,as well as in disease processes,such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I,II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.
Sequence:
MFSLKTLPFLLLLHVQISKAFPVSSKEKNTKTVQDYLEKFYQLPSNQYQSTRKNGTNVIVEKLKEMQRFFGLNVTGKPNEETLDMMKKPRCGVPDSGGFMLTPGNPKWERTNLTYRIRNYTPQLSEAEVERAIKDAFELWSVASPLIFTRISQGEADINIAFYQRDHGDNSPFDGPNGILAHAFQPGQGIGGDAHFDAEETWTNTSANYNLFLVAAHEFGHSLGLAHSSDPGALMYPNYAFRETSNYSLPQDDIDGIQAIYGLSSNPIQPTGPSTPKPCDPSLTFDAITTLRGEILFFKDRYFWRRHPQLQRVEMNFISLFWPSLPTGIQAAYEDFDRDLIFLFKGNQYWALSGYDILQGYPKDISNYGFPSSVQAIDAAVFYRSKTYFFVNDQFWRYDNQRQFMEPGYPKSISGAFPGIESKVDAVFQQEHFFHVFSGPRYYAFDLIAQRVTRVARGNKWLNCRYG

Research Backgrounds

Function:

Can degrade fibrillar type I, II, and III collagens.

Subcellular Location:

Cytoplasmic granule. Secreted>Extracellular space>Extracellular matrix.
Note: Stored in intracellular granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Neutrophils.

Family&Domains:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

Restrictive clause

 

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For Research Use Only.
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