Product: RORG Antibody
Catalog: DF3196
Description: Rabbit polyclonal antibody to RORG
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 58 KD; 58kD(Calculated).
Uniprot: P51449
RRID: AB_2835555

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(90%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(90%)
RORG Antibody detects endogenous levels of total RORG.
Cite Format: Affinity Biosciences Cat# DF3196, RRID:AB_2835555.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


IMD42; MGC129539; NR1F3; Nuclear receptor ROR gamma; Nuclear receptor ROR-gamma; Nuclear receptor RZR gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR related orphan nuclear receptor variant 2; RAR related orphan receptor C; RAR related orphan receptor C, isoform a; RAR related orphan receptor gamma; RAR-related orphan receptor C; Retinoic acid binding receptor gamma; Retinoid related orphan receptor gamma; Retinoid-related orphan receptor-gamma; Rorc; RORG; RORG_HUMAN; RZR GAMMA; RZRG; TOR;



Isoform 1 is widely expressed in many tissues, including liver and adipose, and highly expressed in skeletal muscle. Isoform 2 is primarily expressed in immature thymocytes.




Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51449 As Substrate

Site PTM Type Enzyme
K37 Acetylation
K102 Ubiquitination
S205 Phosphorylation
T225 Phosphorylation
Y264 Phosphorylation
S276 Phosphorylation
K279 Ubiquitination
S339 Phosphorylation

PTMs - P51449 As Enzyme

Substrate Site Source
Q13541 (EIF4EBP1) T36 Uniprot
Q13541 (EIF4EBP1) T45 Uniprot

Research Backgrounds


Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of cellular differentiation, immunity, peripheral circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism. Considered to have intrinsic transcriptional activity, have some natural ligands like oxysterols that act as agonists (25-hydroxycholesterol) or inverse agonists (7-oxygenated sterols), enhancing or repressing the transcriptional activity, respectively. Recruits distinct combinations of cofactors to target gene regulatory regions to modulate their transcriptional expression, depending on the tissue, time and promoter contexts. Regulates the circadian expression of clock genes such as CRY1, ARNTL/BMAL1 and NR1D1 in peripheral tissues and in a tissue-selective manner. Competes with NR1D1 for binding to their shared DNA response element on some clock genes such as ARNTL/BMAL1, CRY1 and NR1D1 itself, resulting in NR1D1-mediated repression or RORC-mediated activation of the expression, leading to the circadian pattern of clock genes expression. Therefore influences the period length and stability of the clock. Involved in the regulation of the rhythmic expression of genes involved in glucose and lipid metabolism, including PLIN2 and AVPR1A. Negative regulator of adipocyte differentiation through the regulation of early phase genes expression, such as MMP3. Controls adipogenesis as well as adipocyte size and modulates insulin sensitivity in obesity. In liver, has specific and redundant functions with RORA as positive or negative modulator of expression of genes encoding phase I and Phase II proteins involved in the metabolism of lipids, steroids and xenobiotics, such as SULT1E1. Also plays also a role in the regulation of hepatocyte glucose metabolism through the regulation of G6PC and PCK1. Regulates the rhythmic expression of PROX1 and promotes its nuclear localization. Plays an indispensable role in the induction of IFN-gamma dependent anti-mycobacterial systemic immunity.

Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes and Peyer's patches. Required for the generation of LTi (lymphoid tissue inducer) cells. Regulates thymocyte survival through DNA-binding on ROREs of target gene promoter regions and recruitment of coactivaros via the AF-2. Also plays a key role, downstream of IL6 and TGFB and synergistically with RORA, for lineage specification of uncommitted CD4(+) T-helper (T(H)) cells into T(H)17 cells, antagonizing the T(H)1 program. Probably regulates IL17 and IL17F expression on T(H) by binding to the essential enhancer conserved non-coding sequence 2 (CNS2) in the IL17-IL17F locus. May also play a role in the pre-TCR activation cascade leading to the maturation of alpha/beta T-cells and may participate in the regulation of DNA accessibility in the TCR-J(alpha) locus.

Subcellular Location:


Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is widely expressed in many tissues, including liver and adipose, and highly expressed in skeletal muscle. Isoform 2 is primarily expressed in immature thymocytes.

Subunit Structure:

Interacts (via AF-2 motif) with the coactivator NCOA2 (via LXXLL motif). Interacts with the corepressor NCOR1 (By similarity). Interacts with CRY1. Interacts (via AF-2 motif) with the coactivators NCOA1 and PPARGC1A (via LXXLL motif) (By similarity). Interacts (via AF-2 motif) with PROX1 (By similarity). Interacts with FOXP3. Interacts with NR0B2 (By similarity).


The AF-2 (activation function-2) motif is required for recruiting coregulators containing LXXLL motifs such as NCOA1 and NCOA2.

Belongs to the nuclear hormone receptor family. NR1 subfamily.

Research Fields

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian rhythm.   (View pathway)


1). Zhou ZY et al. Chronic low-grade inflammation is involved in TLR4 knockout-induced spontaneous obesity in aged mice. BIOMEDICINE & PHARMACOTHERAPY 2022 Jan 29;147:112637. (PubMed: 35093760) [IF=7.5]

2). Li Z et al. Total flavonoids of Sophora flavescens and kurarinone ameliorated ulcerative colitis by regulating Th17/Treg cell homeostasis. JOURNAL OF ETHNOPHARMACOLOGY 2022 Oct 28;297:115500. (PubMed: 35863614) [IF=5.4]

3). Chen Y et al. The Effects of Saposhnikovia divaricata Aqueous Extracts on the Inflammation and Intestinal Microflora in Allergic Rhinitis Mice. Evidence-based Complementary and Alternative Medicine 2022 Oct 14;2022:1052359. (PubMed: 36276863)

Application: WB    Species: Mouse    Sample: nasal mucosa

Figure 4 The effects of SD treatment on the levels of the TLR4/TRAF6/NF-κB and IL-6/ROR-γt/STAT3 pathways' related genes and proteins in the nasal mucosa of AR mice ((n) = 6). (A-E) The levels of TLR4, TRAF6, IL-6, ROR-γt, and STAT3 mRNA were measured by quantitative real-time PCR. (F-M) The levels of TLR4, TRAF6, p-IκBα/IκBα, NF-κB, ROR-γt, and p-STAT3/STAT3 proteins were measured by using the western blot. All error bars represent the mean ± standard deviation. ##P < 0.01, compared to the control group; ∗P < 0.05, ∗∗P < 0.01, compared to the AR group. SD: Saposhnikovia divaricata, AR: allergic rhinitis, TLR4: toll-like receptors 4, TRAF6: TNF receptor-associated factor 6, IL-6: interleukins-6, IκBα: inhibitor of NF-κb, ROR-γt: retinoid-related orphan nuclear receptor γt, and STAT3: signal transducer and activator of transcription 3.

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