Product: ACRBP Antibody
Catalog: DF3353
Description: Rabbit polyclonal antibody to ACRBP
Application: WB IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Dog
Mol.Wt.: 61 KD; 61kD(Calculated).
Uniprot: Q8NEB7
RRID: AB_2835569

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(89%), Bovine(88%), Horse(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
ACRBP Antibody detects endogenous levels of total ACRBP.
RRID:
AB_2835569
Cite Format: Affinity Biosciences Cat# DF3353, RRID:AB_2835569.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACRBP; ACRBP_HUMAN; acrosin binding protein; Acrosin binding protein precursor; Acrosin-binding protein; Cancer/testis antigen 23; Cancer/testis antigen OY TES 1; Cancer/testis antigen OY-TES-1; CT23; FLJ51160; OY TES 1; Proacrosin binding protein sp32; Proacrosin-binding protein sp32; SP32;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8NEB7 ACRBP_HUMAN:

Expression restricted to testis in normal tissue. Expressed in a wide spectrum of cancers, including bladder, breast, liver, lung and colon cancers.

Sequence:
MRKPAAGFLPSLLKVLLLPLAPAAAQDSTQASTPGSPLSPTEYERFFALLTPTWKAETTCRLRATHGCRNPTLVQLDQYENHGLVPDGAVCSNLPYASWFESFCQFTHYRCSNHVYYAKRVLCSQPVSILSPNTLKEIEASAEVSPTTMTSPISPHFTVTERQTFQPWPERLSNNVEELLQSSLSLGGQEQAPEHKQEQGVEHRQEPTQEHKQEEGQKQEEQEEEQEEEGKQEEGQGTKEGREAVSQLQTDSEPKFHSESLSSNPSSFAPRVREVESTPMIMENIQELIRSAQEIDEMNEIYDENSYWRNQNPGSLLQLPHTEALLVLCYSIVENTCIITPTAKAWKYMEEEILGFGKSVCDSLGRRHMSTCALCDFCSLKLEQCHSEASLQRQQCDTSHKTPFVSPLLASQSLSIGNQVGSPESGRFYGLDLYGGLHMDFWCARLATKGCEDVRVSGWLQTEFLSFQDGDFPTKICDTDYIQYPNYCSFKSQQCLMRNRNRKVSRMRCLQNETYSALSPGKSEDVVLRWSQEFSTLTLGQFG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Horse
88
Bovine
88
Dog
88
Rabbit
63
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NEB7 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation
S141 Phosphorylation
S145 Phosphorylation

Research Backgrounds

Function:

May be involved in packaging and condensation of the acrosin zymogen in the acrosomal matrix via its association with proacrosin.

PTMs:

The N-terminus is blocked.

Phosphorylated on Tyr residues in capacitated sperm.

Subcellular Location:

Secreted. Cytoplasmic vesicle>Secretory vesicle>Acrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expression restricted to testis in normal tissue. Expressed in a wide spectrum of cancers, including bladder, breast, liver, lung and colon cancers.

Subunit Structure:

Binds proacrosin.

Restrictive clause

 

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