PPHLN Antibody - #DF3355

(2)  
Product: PPHLN Antibody
Catalog: DF3355
Description: Rabbit polyclonal antibody to PPHLN
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 53 KD; 53kD(Calculated).
Uniprot: Q8NEY8
RRID: AB_2835571

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
PPHLN Antibody detects endogenous levels of total PPHLN.
RRID:
AB_2835571
Cite Format: Affinity Biosciences Cat# DF3355, RRID:AB_2835571.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Gastric cancer antigen Ga50; Periphilin 1; Periphilin-1; PPHLN_HUMAN; PPHLN1;

Immunogens

Immunogen:
Uniprot:

Q8NEY8(PPHLN_HUMAN) >>Visit HPA database.

Gene(ID):
PPHLN1(51535)
Expression:
Q8NEY8 PPHLN_HUMAN:

Ubiquitous.

Sequence:
MWSEGRYEYERIPRERAPPRSHPSDGYNRLVNIVPKKPPLLDRPGEGSYNRYYSHVDYRDYDEGRSFSHDRRSGPPHRGDESGYRWTRDDHSASRQPEYRDMRDGFRRKSFYSSHYARERSPYKRDNTFFRESPVGRKDSPHSRSGSSVSSRSYSPERSKSYSFHQSQHRKSVRPGASYKRQNEGNPERDKERPVQSLKTSRDTSPSSGSAVSSSKVLDKPSRLTEKELAEAASKWAAEKLEKSDESNLPEISEYEAGSTAPLFTDQPEEPESNTTHGIELFEDSQLTTRSKAIASKTKEIEQVYRQDCETFGMVVKMLIEKDPSLEKSIQFALRQNLHEIESAGQTWQQVPPVRNTEMDHDGTPENEGEETAQSAPQPPQAPQPLQPRKKRVRRTTQLRRTTGAPDITWGMLKKTTQEAERILLRTQTPFTPENLFLAMLSVVHCNSRKDVKPENKQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Dog
100
Rabbit
100
Pig
92
Chicken
83
Zebrafish
50
Xenopus
42
Horse
0
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NEY8 As Substrate

Site PTM Type Enzyme
Y9 Phosphorylation
Y27 Phosphorylation
K37 Sumoylation
K37 Ubiquitination
R43 Methylation
S48 Phosphorylation
Y49 Phosphorylation
Y52 Phosphorylation
Y53 Phosphorylation
S54 Phosphorylation
Y58 Phosphorylation
Y61 Phosphorylation
S68 Phosphorylation
S73 Phosphorylation
R78 Methylation
S92 Phosphorylation
S94 Phosphorylation
Y99 Phosphorylation
K109 Sumoylation
S110 Phosphorylation Q96GD4 (AURKB)
Y112 Phosphorylation
S113 Phosphorylation
S114 Phosphorylation
Y116 Phosphorylation
S121 Phosphorylation
T128 Phosphorylation
S133 Phosphorylation
S140 Phosphorylation
S143 Phosphorylation
S145 Phosphorylation
S153 Phosphorylation
S155 Phosphorylation
K160 Ubiquitination
S161 Phosphorylation
Y162 Phosphorylation
S163 Phosphorylation
S167 Phosphorylation
K171 Sumoylation
S172 Phosphorylation
S178 Phosphorylation
K180 Acetylation
S197 Phosphorylation
K199 Ubiquitination
T200 Phosphorylation
S201 Phosphorylation
T204 Phosphorylation
S205 Phosphorylation
S207 Phosphorylation
S208 Phosphorylation
S210 Phosphorylation
S214 Phosphorylation
K220 Ubiquitination
S222 Phosphorylation
K227 Acetylation
K227 Ubiquitination
K235 Acetylation
K235 Sumoylation
K235 Ubiquitination
K240 Acetylation
K240 Sumoylation
K240 Ubiquitination
S244 Phosphorylation
S247 Phosphorylation
Y255 Phosphorylation
T298 Phosphorylation
K299 Ubiquitination
K322 Ubiquitination
S325 Phosphorylation
K328 Sumoylation
K328 Ubiquitination
S329 Phosphorylation
K453 Sumoylation

Research Backgrounds

Function:

Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin. Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex. The HUSH complex is also involved in the silencing of unintegrated retroviral DNA: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed. May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation.

PTMs:

Substrate of transglutaminase (in vitro).

Subcellular Location:

Nucleus. Cytoplasm. Chromosome.
Note: In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions (PubMed:12853457). Localizes to chromatin (PubMed:26022416).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer. Component of the HUSH complex; at least composed of TASOR, PPHLN1 and MPHOSPH8. Interacts with SIN3A and HDAC1. Interacts with PPL.

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