Product: ZNF397 Antibody
Catalog: DF3356
Description: Rabbit polyclonal antibody to ZNF397
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Horse
Mol.Wt.: 61 KD; 61kD(Calculated).
Uniprot: Q8NF99
RRID: AB_2835572

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(82%), Horse(82%)
Clonality:
Polyclonal
Specificity:
ZNF397 Antibody detects endogenous levels of total ZNF397.
RRID:
AB_2835572
Cite Format: Affinity Biosciences Cat# DF3356, RRID:AB_2835572.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2810411K16Rik; MGC13250; Zinc finger and SCAN domain containing protein 15; Zinc finger and SCAN domain-containing protein 15; Zinc finger protein 397; Zinc finger protein 47; ZN397_HUMAN; ZNF 397; ZNF 47; ZNF397; ZNF47; ZSCAN 15; ZSCAN15;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8NF99 ZN397_HUMAN:

Expressed strongly in testis, moderately in skeletal muscle, pancreas and prostate, and weakly in heart, placenta, liver, kidney, spleen, thymus and small intestine.

Sequence:
MAVESGVISTLIPQDPPEQELILVKVEDNFSWDEKFKQNGSTQSCQELFRQQFRKFCYQETPGPREALSRLQELCYQWLMPELHTKEQILELLVLEQFLSILPEELQIWVQQHNPESGEEAVTLLEDLEREFDDPGQQVPASPQGPAVPWKDLTCLRASQESTDIHLQPLKTQLKSWKPCLSPKSDCENSETATKEGISEEKSQGLPQEPSFRGISEHESNLVWKQGSATGEKLRSPSQGGSFSQVIFTNKSLGKRDLYDEAERCLILTTDSIMCQKVPPEERPYRCDVCGHSFKQHSSLTQHQRIHTGEKPYKCNQCGKAFSLRSYLIIHQRIHSGEKAYECSECGKAFNQSSALIRHRKIHTGEKACKCNECGKAFSQSSYLIIHQRIHTGEKPYECNECGKTFSQSSKLIRHQRIHTGERPYECNECGKAFRQSSELITHQRIHSGEKPYECSECGKAFSLSSNLIRHQRIHSGEEPYQCNECGKTFKRSSALVQHQRIHSGDEAYICNECGKAFRHRSVLMRHQRVHTIK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
82
Horse
82
Sheep
73
Dog
73
Rabbit
73
Bovine
64
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NF99 As Substrate

Site PTM Type Enzyme
S31 Phosphorylation
K35 Ubiquitination
K37 Ubiquitination
K55 Ubiquitination
S142 Phosphorylation
S159 Phosphorylation
S176 Phosphorylation
S182 Phosphorylation
S185 Phosphorylation
S190 Phosphorylation
K202 Ubiquitination
S203 Phosphorylation
S211 Phosphorylation
K225 Ubiquitination
T230 Phosphorylation
S236 Phosphorylation
S298 Phosphorylation
T308 Phosphorylation
K339 Ubiquitination
K367 Acetylation
T392 Phosphorylation
K395 Ubiquitination
K432 Ubiquitination
K451 Ubiquitination
S466 Phosphorylation
K516 Ubiquitination

Research Backgrounds

Function:

Isoform 3 acts as a DNA-dependent transcriptional repressor.

Subcellular Location:

Nucleus.

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed strongly in testis, moderately in skeletal muscle, pancreas and prostate, and weakly in heart, placenta, liver, kidney, spleen, thymus and small intestine.

Subunit Structure:

Isoforms 1 and 3 can both homo- and hetero-associate. Homo-association of isoform 1 is dependent on the presence of the SCAN domain.

Family&Domains:

Belongs to the krueppel C2H2-type zinc-finger protein family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.