hnRNP F Antibody - #DF3204

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Product: hnRNP F Antibody
Catalog: DF3204
Description: Rabbit polyclonal antibody to hnRNP F
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 48 KD; 46kD(Calculated).
Uniprot: P52597
RRID: AB_2835584

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
hnRNP F Antibody detects endogenous levels of total hnRNP F.
RRID:
AB_2835584
Cite Format: Affinity Biosciences Cat# DF3204, RRID:AB_2835584.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Heterogeneous nuclear ribonucleoprotein F; hnRNP F; HnRNP F protein; Hnrnpf; HNRPF; HNRPF_HUMAN; mcs94 1; MGC110997; N-terminally processed; Nucleolin like protein; Nucleolin like protein mcs94 1; Nucleolin-like protein mcs94-1; OK/SW-cl.23; OTTHUMP00000019482; OTTHUMP00000043413; OTTHUMP00000043414; Ribonucleoprotein F;

Immunogens

Immunogen:
Uniprot:

P52597(HNRPF_HUMAN) >>Visit HPA database.

Gene(ID):
HNRNPF(3185)
Expression:
P52597 HNRPF_HUMAN:

Expressed ubiquitously.

Sequence:
MMLGPEGGEGFVVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMALKKDRESMGHRYIEVFKSHRTEMDWVLKHSGPNSADSANDGFVRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEEVRSYSDPPLKFMSVQRPGPYDRPGTARRYIGIVKQAGLERMRPGAYSTGYGGYEEYSGLSDGYGFTTDLFGRDLSYCLSGMYDHRYGDSEFTVQSTTGHCVHMRGLPYKATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEEAVAAMSKDRANMQHRYIELFLNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNSMGGYD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52597 As Substrate

Site PTM Type Enzyme
M1 Acetylation
M2 Acetylation
K14 Ubiquitination
S23 Phosphorylation
S63 Phosphorylation
K68 Sumoylation
K72 Sumoylation
S77 Phosphorylation
Y82 Phosphorylation
K87 Acetylation
K87 Methylation
K87 Ubiquitination
R90 Methylation
K98 Acetylation
K98 Ubiquitination
S104 Phosphorylation
S107 Phosphorylation
R114 Methylation
T142 Phosphorylation
K150 Sumoylation
T152 Phosphorylation
S161 Phosphorylation
K167 Ubiquitination
K171 Ubiquitination
Y180 Phosphorylation
K185 Sumoylation
K185 Ubiquitination
S186 Phosphorylation
S187 Phosphorylation
S193 Phosphorylation
S195 Phosphorylation
K200 Sumoylation
K200 Ubiquitination
S203 Phosphorylation
Y210 Phosphorylation
R212 Methylation
T215 Phosphorylation
R217 Methylation
R218 Methylation
Y219 Phosphorylation
K224 Acetylation
K224 Methylation
K224 Sumoylation
K224 Ubiquitination
R232 Methylation
Y236 Phosphorylation
Y240 Phosphorylation
Y243 Phosphorylation
Y246 Phosphorylation
S247 Phosphorylation
Y253 Phosphorylation
S265 Phosphorylation
Y266 Phosphorylation
C267 S-Nitrosylation
S269 Phosphorylation
Y272 Phosphorylation
Y276 Phosphorylation
S285 Phosphorylation
T286 Phosphorylation
K299 Ubiquitination
T301 Phosphorylation
Y306 Phosphorylation
S310 Phosphorylation
K347 Ubiquitination
Y401 Phosphorylation

Research Backgrounds

Function:

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.

PTMs:

Sumoylated.

Subcellular Location:

Nucleus>Nucleoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed ubiquitously.

Subunit Structure:

Identified in the spliceosome C complex. Interacts with AGO1, AGO2, TBP and TXNL4/DIM1.

Family&Domains:

The N-terminal RRM domains are responsible for recognizing the G-tract of BCL-X RNA.

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