PON1 Recombinant Rabbit mAb - #BF4128

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Product: PON1 Recombinant Rabbit mAb
Catalog: BF4128
Description: Rabbit monoclonal antibody to PON1
Application: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 40 kDa(Observed); 40kD(Calculated).
Uniprot: P27169

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IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse, Rat
Clonality:
Monoclonal [ReFirm23575]
Specificity:
PON1 Recombinant Rabbit mAb detects endogenous levels of PON1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A esterase 1; A-esterase 1; Aromatic esterase 1; Arylesterase 1; Arylesterase B type; ESA; Esterase A; K 45; K-45; MVCD5; Paraoxonase 1; Paraoxonase; Paraoxonase B type; Paraoxonase, plasma; Paraoxonase1; PON 1; PON; PON1; PON1_HUMAN; Serum aryldiakylphosphatase; Serum aryldialkylphosphatase 1; Serum paraoxonase/arylesterase 1;

Immunogens

Immunogen:

A synthetic peptide from human PON1

Uniprot:

P27169(PON1_HUMAN) >>Visit HPA database.

Gene(ID):
PON1(5444)
Expression:
P27169 PON1_HUMAN:

Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.

Description:
This gene encodes a member of the paraoxonase family of enzymes and exhibits lactonase and ester hydrolase activity. Following synthesis in the kidney and liver,the enzyme is secreted into the circulation,where it binds to high density lipoprotein (HDL) particles and hydrolyzes thiolactones and xenobiotics,including paraoxon,a metabolite of the insecticide parathion. Polymorphisms in this gene may be associated with coronary artery disease and diabetic retinopathy. The gene is found in a cluster of three related paraoxonase genes on chromosome 7.
Sequence:
MAKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEEKSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLDPYLQSWEMYLGLAWSYVVYYSPSEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEKHANWTLTPLKSLDFNTLVDNISVDPETGDLWVGCHPNGMKIFFYDSENPPASEVLRIQNILTEEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKALYCEL

Research Backgrounds

Function:

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.

PTMs:

Glycosylated.

The signal sequence is not cleaved.

Present in two forms, form B contains a disulfide bond, form A does not.

Subcellular Location:

Secreted>Extracellular space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas.

Family&Domains:

Belongs to the paraoxonase family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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