alpha 5 Defensin Recombinant Rabbit mAb - #BF4161

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Product: alpha 5 Defensin Recombinant Rabbit mAb
Catalog: BF4161
Description: Rabbit monoclonal antibody to alpha 5 Defensin
Application: WB IHC
Reactivity: Human
Mol.Wt.: 10 kDa(Observed); 10kD(Calculated).
Uniprot: Q01523

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:3000, IHC 1:200-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [ReFirm23608]
Specificity:
alpha 5 Defensin Recombinant Rabbit mAb detects endogenous levels of alpha 5 Defensin.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

alpha 5; DEF5; DEF5_HUMAN; Defa; Defa29; DEFA5; Defcr29; Defcr5; Defensin 5; Defensin alpha 5; Defensin alpha 5 Paneth cell specific; Defensin alpha 5 preproprotein; Defensin; Enteric defensin; HD 5; HD5; HD5(20-94); HD5(63-94); MGC129728; RD 5;

Immunogens

Immunogen:

A synthetic peptide from human alpha 5 Defensin

Uniprot:

Q01523(DEF5_HUMAN) >>Visit HPA database.

Gene(ID):
DEFA5(1670)
Expression:
Q01523 DEF5_HUMAN:

Paneth cells of the small intestine (at protein level).

Description:
Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine,respiratory tract,urinary tract,and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene,defensin,alpha 5,is highly expressed in the secretory granules of Paneth cells of the ileum.
Sequence:
MRTIAILAAILLVALQAQAESLQERADEATTQKQSGEDNQDLAISFAGNGLSALRTSGSQARATCYCRTGRCATRESLSGVCEISGRLYRLCCR

Research Backgrounds

Function:

Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.

PTMs:

Glycosylated.

Subcellular Location:

Secreted. Cytoplasmic vesicle>Secretory vesicle.
Note: Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Paneth cells of the small intestine (at protein level).

Family&Domains:

Belongs to the alpha-defensin family.

Restrictive clause

 

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