Product: PTTG Antibody
Catalog: DF3213
Description: Rabbit polyclonal antibody to PTTG
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 20 KD; 20kD(Calculated).
Uniprot: P53801
RRID: AB_2835593

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(89%), Dog(89%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
PTTG Antibody detects endogenous levels of total PTTG.
RRID:
AB_2835593
Cite Format: Affinity Biosciences Cat# DF3213, RRID:AB_2835593.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1810010L20Rik; AI314311; AU018448; C21orf1; C21orf3; C79540; chromosome 21 open reading frame 1; MGC36923; PBF; Pituitary tumor transforming 1 interacting protein; Pituitary tumor transforming gene 1 protein interacting protein; Pituitary tumor transforming gene protein binding factor; Pituitary tumor-transforming gene 1 protein-interacting protein; Pituitary tumor-transforming gene protein-binding factor; PTTG binding factor; PTTG-binding factor; PTTG_HUMAN; Pttg1ip; Putative surface glycoprotein C21orf1 precursor;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P53801 PTTG_HUMAN:

Ubiquitous.

Sequence:
MAPGVARGPTPYWRLRLGGAALLLLLIPVAAAQEPPGAACSQNTNKTCEECLKNVSCLWCNTNKACLDYPVTSVLPPASLCKLSSARWGVCWVNFEALIITMSVVGGTLLLGIAICCCCCCRRKRSRKPDRSEEKAMREREERRIRQEERRAEMKTRHDEIRKKYGLFKEENPYARFENN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Horse
89
Bovine
89
Sheep
89
Dog
89
Chicken
89
Rabbit
89
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53801 As Substrate

Site PTM Type Enzyme
K135 Ubiquitination
T156 Phosphorylation
K164 Ubiquitination
Y165 Phosphorylation
K169 Sumoylation
K169 Ubiquitination
Y174 Phosphorylation P12931 (SRC)

Research Backgrounds

Function:

May facilitate PTTG1 nuclear translocation.

Subcellular Location:

Membrane>Single-pass type I membrane protein. Cytoplasm. Nucleus.
Note: According to PubMed:10781616, it is found in the cytoplasm and the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with PTTG1.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.