AF10 Antibody - #DF3216
| Product: | AF10 Antibody |
| Catalog: | DF3216 |
| Description: | Rabbit polyclonal antibody to AF10 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 109 KD; 113kD(Calculated). |
| Uniprot: | P55197 |
| RRID: | AB_2835596 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3216, RRID:AB_2835596.
Fold/Unfold
AF 10; AF10; AF10_HUMAN; ALL 1 fused gene from chromosome 10; ALL1 fused gene from chromosome 10; ALL1-fused gene from chromosome 10 protein; DKFZp686E10210; MGC75086; MLLT 10; MLLT10; Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 10; Myeloid/lymphoid or mixed lineage leukemia translocated to 10; OTTHUMP00000045273; Protein AF 10; Protein AF-10; Protein AF10; Type I AF10 protein;
Immunogens
- P55197 AF10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVSSDRPVSLEDEVSHSMKEMIGGCCVCSDERGWAENPLVYCDGHGCSVAVHQACYGIVQVPTGPWFCRKCESQERAARVRCELCPHKDGALKRTDNGGWAHVVCALYIPEVQFANVSTMEPIVLQSVPHDRYNKTCYICDEQGRESKAATGACMTCNKHGCRQAFHVTCAQFAGLLCEEEGNGADNVQYCGYCKYHFSKLKKSKRGSNRSYDQSLSDSSSHSQDKHHEKEKKKYKEKDKHKQKHKKQPEPSPALVPSLTVTTEKTYTSTSNNSISGSLKRLEDTTARFTNANFQEVSAHTSSGKDVSETRGSEGKGKKSSAHSSGQRGRKPGGGRNPGTTVSAASPFPQGSFSGTPGSVKSSSGSSVQSPQDFLSFTDSDLRNDSYSHSQQSSATKDVHKGESGSQEGGVNSFSTLIGLPSTSAVTSQPKSFENSPGDLGNSSLPTAGYKRAQTSGIEEETVKEKKRKGNKQSKHGPGRPKGNKNQENVSHLSVSSASPTSSVASAAGSITSSSLQKSPTLLRNGSLQSLSVGSSPVGSEISMQYRHDGACPTTTFSELLNAIHNGIYNSNDVAVSFPNVVSGSGSSTPVSSSHLPQQSSGHLQQVGALSPSAVSSAAPAVATTQANTLSGSSLSQAPSHMYGNRSNSSMAALIAQSENNQTDQDLGDNSRNLVGRGSSPRGSLSPRSPVSSLQIRYDQPGNSSLENLPPVAASIEQLLERQWSEGQQFLLEQGTPSDILGMLKSLHQLQVENRRLEEQIKNLTAKKERLQLLNAQLSVPFPTITANPSPSHQIHTFSAQTAPTTDSLNSSKSPHIGNSFLPDNSLPVLNQDLTSSGQSTSSSSALSTPPPAGQSPAQQGSGVSGVQQVNGVTVGALASGMQPVTSTIPAVSAVGGIIGALPGNQLAINGIVGALNGVMQTPVTMSQNPTPLTHTTVPPNATHPMPATLTNSASGLGLLSDQQRQILIHQQQFQQLLNSQQLTPEQHQAFLYQLMQHHHQQHHQPELQQLQIPGPTQIPINNLLAGTQAPPLHTATTNPFLTIHGDNASQKVARLSDKTGPVAQEKS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P55197 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S147 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| K236 | Acetylation | Uniprot | |
| K240 | Acetylation | Uniprot | |
| K242 | Acetylation | Uniprot | |
| K246 | Acetylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S269 | O-Glycosylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| T270 | Phosphorylation | Uniprot | |
| S271 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| K280 | Methylation | Uniprot | |
| K280 | Sumoylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| S404 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| K451 | Acetylation | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| S527 | Phosphorylation | Uniprot | |
| S532 | Phosphorylation | Uniprot | |
| S679 | Phosphorylation | Uniprot | |
| S680 | Phosphorylation | Uniprot | |
| S684 | Phosphorylation | Uniprot | |
| S686 | Phosphorylation | Uniprot | |
| S689 | Phosphorylation | Uniprot | |
| S692 | Phosphorylation | Uniprot |
Research Backgrounds
Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA. In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation.
Nucleus.
Expressed abundantly in testis.
Self-associates. Interacts with FSTL3 isoform 2; the interaction enhances MLLT10 in vitro transcriptional activity and self-association. Interacts with YEATS4. Interacts with SS18. Interacts with DOT1L; this interaction also occurs with the KMT2A/MLL1 fusion protein. Interacts with histone H3; interaction is necessary for MLLT10 binding to nucleosomes; interaction is inhibited by histone H3 'Lys-27' methylations (H3K27me1, H3K27me2 and H3K27me3) amd acetylation; interaction stabilizes association of MLLT10 at chromatin; interaction is essential for histone H3 'Lys-79' dimethylation (H3K79me2).
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