TRAF4 Recombinant Rabbit mAb - #BF4212

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Product: TRAF4 Recombinant Rabbit mAb
Catalog: BF4212
Description: Rabbit monoclonal antibody to TRAF4
Application: WB
Reactivity: Human, Mouse
Mol.Wt.: 54 kDa(Observed); 54kD(Calculated).
Uniprot: Q9BUZ4

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Data Sheet
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Protocols
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IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse
Clonality:
Monoclonal [ReFirm23659]
Specificity:
TRAF4 Recombinant Rabbit mAb detects endogenous levels of TRAF4.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CART 1; CART1; cysteine rich domain associated with ring and TRAF domain; Cysteine rich domain associated with RING and Traf domains protein 1; Cysteine-rich domain associated with RING and Traf domains protein 1; Malignant 62; Metastatic lymph node gene 62 protein; MLN 62; MLN62; RING finger protein 83; RNF 83; RNF83; TNF receptor associated factor 4; TNF receptor-associated factor 4; TRAF 4; Traf4; TRAF4_HUMAN; tumor necrosis receptor-associated factor 4A;

Immunogens

Immunogen:

A synthetic peptide from human TRAF4

Uniprot:

Q9BUZ4(TRAF4_HUMAN) >>Visit HPA database.

Gene(ID):
TRAF4(9618)
Expression:
Q9BUZ4 TRAF4_HUMAN:

Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.

Description:
This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with,and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor,p75 (NTR,NTSR1),and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox,a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus,is thought to be involved in the oxidative activation of MAPK8,JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined.
Sequence:
MPGFDYKFLEKPKRRLLCPLCGKPMREPVQVSTCGHRFCDTCLQEFLSEGVFKCPEDQLPLDYAKIYPDPELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCPNRCPMKLSRRDLPAHLQHDCPKRRLKCEFCGCDFSGEAYESHEGMCPQESVYCENKCGARMMRRLLAQHATSECPKRTQPCTYCTKEFVFDTIQSHQYQCPRLPVACPNQCGVGTVAREDLPGHLKDSCNTALVLCPFKDSGCKHRCPKLAMARHVEESVKPHLAMMCALVSRQRQELQELRRELEELSVGSDGVLIWKIGSYGRRLQEAKAKPNLECFSPAFYTHKYGYKLQVSAFLNGNGSGEGTHLSLYIRVLPGAFDNLLEWPFARRVTFSLLDQSDPGLAKPQHVTETFHPDPNWKNFQKPGTWRGSLDESSLGFGYPKFISHQDIRKRNYVRDDAVFIRAAVELPRKILS

Research Backgrounds

Function:

Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions.

PTMs:

Polyubiquitinated, leading to its proteasomal degradation.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Perinuclear region. Cell junction>Tight junction. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.

Family&Domains:

The coiled coil domain mediates homo- and hetero-oligomerization.

The MATH/TRAF domain binds to receptor cytoplasmic domains.

Belongs to the TNF receptor-associated factor family. B subfamily.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

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For Research Use Only.
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