ELL Antibody - #DF3217
| Product: | ELL Antibody |
| Catalog: | DF3217 |
| Description: | Rabbit polyclonal antibody to ELL |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Dog, Chicken, Xenopus |
| Mol.Wt.: | 65 KD; 68kD(Calculated). |
| Uniprot: | P55199 |
| RRID: | AB_2835597 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3217, RRID:AB_2835597.
Fold/Unfold
eleven nineteen lysine rich leukemia gene; Eleven-nineteen lysine-rich leukemia protein; ELL1; elongation factor RNA polymerase II; Men; RNA polymerase II elongation factor;
Immunogens
Expressed in all tissues tested. Highest levels found in placenta, skeletal muscle, testis and peripheral blood leukocytes.
- P55199 ELL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAALKEDRSYGLSCGRVSDGSKVSVFHVKLTDSALRAFESYRARQDSVSLRPSIRFQGSQGHISIPQPDCPAEARTFSFYLSNIGRDNPQGSFDCIQQYVSSHGEVHLDCLGSIQDKITVCATDDSYQKARQSMAQAEEETRSRSAIVIKAGGRYLGKKVQFRKPAPGATDAVPSRKRATPINLASAIRKSGASAVSGGSGVSQRPFRDRVLHLLALRPYRKAELLLRLQKDGLTQADKDALDGLLQQVANMSAKDGTCTLQDCMYKDVQKDWPGYSEGDQQLLKRVLVRKLCQPQSTGSLLGDPAASSPPGERGRSASPPQKRLQPPDFIDPLANKKPRISHFTQRAQPAVNGKLGVPNGREALLPTPGPPASTDTLSSSTHLPPRLEPPRAHDPLADVSNDLGHSGRDCEHGEAAAPAPTVRLGLPLLTDCAQPSRPHGSPSRSKPKKKSKKHKDKERAAEDKPRAQLPDCAPATHATPGAPADTPGLNGTCSVSSVPTSTSETPDYLLKYAAISSSEQRQSYKNDFNAEYSEYRDLHARIERITRRFTQLDAQLRQLSQGSEEYETTRGQILQEYRKIKKTNTNYSQEKHRCEYLHSKLAHIKRLIAEYDQRQLQAWP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P55199 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| Y10 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| T123 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| Y127 | Phosphorylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| T180 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| T258 | Phosphorylation | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| Y266 | Phosphorylation | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S317 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| T431 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S442 | Phosphorylation | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| K451 | Methylation | Uniprot | |
| K456 | Methylation | Uniprot | |
| K465 | Methylation | Uniprot | |
| S519 | Phosphorylation | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| K583 | Ubiquitination | Uniprot |
Research Backgrounds
Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription. ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation.
Nucleus. Nucleus speckle. Nucleus>Cajal body.
Note: Colocalizes with EAF2 to nuclear speckles (PubMed:12446457). Colocalizes with coilin in subnuclear cajal and histone locus bodies (PubMed:12686606). Translocates in the LEC complex to cajal and histone locus bodies at snRNA genes in a ICE1-dependent manner. Associates to transcriptionally active chromatin at snRNA genes(PubMed:23932780).
Expressed in all tissues tested. Highest levels found in placenta, skeletal muscle, testis and peripheral blood leukocytes.
Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Component of the little elongation complex (LEC), at least composed of ELL (ELL, ELL2 or ELL3), ZC3H8, ICE1 and ICE2. Interacts with AFF4; the interaction is direct. Interacts with EAF1 and EAF2. Interacts with ICE1 (via N-terminus domain). Interacts with ICE2. Interacts with USPL1.
Belongs to the ELL/occludin family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.