ST5 Antibody - #DF3226

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Product: ST5 Antibody
Catalog: DF3226
Description: Rabbit polyclonal antibody to ST5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 126 KD; 126kD(Calculated).
Uniprot: P78524
RRID: AB_2835606

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(90%), Horse(100%), Sheep(90%), Rabbit(100%), Dog(91%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
ST5 Antibody detects endogenous levels of total ST5.
RRID:
AB_2835606
Cite Format: Affinity Biosciences Cat# DF3226, RRID:AB_2835606.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DENND2B; DENN domain containing protein 2B; DENN domain-containing protein 2B; DENN/MADD domain containing 2B; HeLa tumor suppression 1; HTS1; p126; ST 5; ST5; ST5_HUMAN; Suppression of tumorigenicity 5; Suppression of tumorigenicity 5 protein;

Immunogens

Immunogen:
Uniprot:

P78524(DEN2B_HUMAN) >>Visit HPA database.

Gene(ID):
ST5(6764)
Expression:
P78524 DEN2B_HUMAN:

Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level).

Sequence:
MTMTANKNSSITHGAGGTKAPRGTLSRSQSVSPPPVLSPPRSPIYPLSDSETSACRYPSHSSSRVLLKDRHPPAPSPQNPQDPSPDTSPPTCPFKTASFGYLDRSPSACKRDAQKESVQGAAQDVAGVAACLPLAQSTPFPGPAAGPRGVLLTRTGTRAHSLGIREKISAWEGRREASPRMSMCGEKREGSGSEWAASEGCPSLGCPSVVPSPCSSEKTFDFKGLRRMSRTFSECSYPETEEEGEALPVRDSFYRLEKRLGRSEPSAFLRGHGSRKESSAVLSRIQKIEQVLKEQPGRGLPQLPSSCYSVDRGKRKTGTLGSLEEPAGGASVSAGSRAVGVAGVAGEAGPPPEREGSGSTKPGTPGNSPSSQRLPSKSSLDPAVNPVPKPKRTFEYEADKNPKSKPSNGLPPSPTPAAPPPLPSTPAPPVTRRPKKDMRGHRKSQSRKSFEFEDASSLQSLYPSSPTENGTENQPKFGSKSTLEENAYEDIVGDLPKENPYEDVDLKSRRAGRKSQQLSENSLDSLHRMWSPQDRKYNSPPTQLSLKPNSQSLRSGNWSERKSHRLPRLPKRHSHDDMLLLAQLSLPSSPSSLNEDSLSTTSELLSSRRARRIPKLVQRINSIYNAKRGKKRLKKLSMSSIETASLRDENSESESDSDDRFKAHTQRLVHIQSMLKRAPSYRTLELELLEWQERELFEYFVVVSLKKKPSRNTYLPEVSYQFPKLDRPTKQMREAEERLKAIPQFCFPDAKDWLPVSEYSSETFSFMLTGEDGSRRFGYCRRLLPSGKGPRLPEVYCVISRLGCFGLFSKVLDEVERRRGISAALVYPFMRSLMESPFPAPGKTIKVKTFLPGAGNEVLELRRPMDSRLEHVDFECLFTCLSVRQLIRIFASLLLERRVIFVADKLSTLSSCSHAVVALLYPFSWQHTFIPVLPASMIDIVCCPTPFLVGLLSSSLPKLKELPVEEALMVNLGSDRFIRQMDDEDTLLPRKLQAALEQALERKNELISQDSDSDSDDECNTLNGLVSEVFIRFFVETVGHYSLFLTQSEKGERAFQREAFRKSVASKSIRRFLEVFMESQMFAGFIQDRELRKCRAKGLFEQRVEQYLEELPDTEQSGMNKFLRGLGNKMKFLHKKN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Dog
91
Bovine
90
Sheep
90
Xenopus
89
Pig
73
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78524 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
T12 Phosphorylation
K19 Ubiquitination
S28 Phosphorylation
S30 Phosphorylation
S32 Phosphorylation
S48 Phosphorylation
S76 Phosphorylation
S84 Phosphorylation
K95 Methylation
S98 Phosphorylation
S105 Phosphorylation
S107 Phosphorylation
S161 Phosphorylation
S182 Phosphorylation
S191 Phosphorylation
S193 Phosphorylation
S198 Phosphorylation
S203 Phosphorylation
S212 Phosphorylation
S233 Phosphorylation
S236 Phosphorylation
T240 Phosphorylation
Y254 Phosphorylation
R270 Methylation
S279 Phosphorylation
Y308 Phosphorylation
S309 Phosphorylation
S322 Phosphorylation
S357 Phosphorylation
T364 Phosphorylation
S368 Phosphorylation
S376 Phosphorylation
K377 Acetylation
K389 Acetylation
K391 Acetylation
Y396 Phosphorylation
S413 Phosphorylation
S449 Phosphorylation
S464 Phosphorylation
S465 Phosphorylation
T467 Phosphorylation
Y488 Phosphorylation
Y501 Phosphorylation
S515 Phosphorylation
S519 Phosphorylation
S522 Phosphorylation
S525 Phosphorylation
S531 Phosphorylation
Y537 Phosphorylation
S539 Phosphorylation
S552 Phosphorylation
S574 Phosphorylation
S622 Phosphorylation
S637 Phosphorylation
S673 Phosphorylation
K676 Methylation
T844 Phosphorylation
Y1041 Phosphorylation
S1042 Phosphorylation
K1097 Ubiquitination

Research Backgrounds

Function:

May be involved in cytoskeletal organization and tumorogenicity. Seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Plays a role in EGFR trafficking from recycling endosomes back to the cell membrane.

Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.

May block ERK2 activation stimulated by ABL1 (Probable). May alter cell morphology and cell growth (Probable).

PTMs:

Phosphorylated. Phosphorylation decreases ITSN1 binding.

Subcellular Location:

Cytoplasm>Cell cortex. Cell membrane. Recycling endosome.
Note: Colocalizes with RAB13 and ITSN1 at cytoplasmic vesicles that are most likely recycling endosomes. Colocalizes with the cortical actin cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level).

Subunit Structure:

Interacts with ITSN1 and GRB2. Isoform 1 interacts with the SH3 domain of ABL1.

Restrictive clause

 

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