SFRS3 Antibody - #DF3230

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Product: SFRS3 Antibody
Catalog: DF3230
Description: Rabbit polyclonal antibody to SFRS3
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 25 KD; 19kD(Calculated).
Uniprot: P84103
RRID: AB_2835610

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
SFRS3 Antibody detects endogenous levels of total SFRS3.
RRID:
AB_2835610
Cite Format: Affinity Biosciences Cat# DF3230, RRID:AB_2835610.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

arginine/serine-rich 3; Pre mRNA splicing factor SRP20; Pre-mRNA-splicing factor SRP20; Serine/arginine-rich splicing factor 3; Splicing factor; Splicing factor arginine/serine rich 20 kD; Splicing factor arginine/serine rich 3; SRp20; SRSF3; SRSF3_HUMAN; X16;

Immunogens

Immunogen:
Uniprot:

P84103(SRSF3_HUMAN) >>Visit HPA database.

Gene(ID):
SRSF3(6428)
Sequence:
MHRDSCPLDCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKRSRNRGPPPSWGRRPRDDYRRRSPPPRRRSPRRRSFSRSRSRSLSRDRRRERSLSRERNHKPSRSFSRSRSRSRSNERK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P84103 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
C6 S-Nitrosylation
C10 S-Nitrosylation
K11 Acetylation
K11 Ubiquitination
Y13 Phosphorylation
K23 Acetylation
K23 Ubiquitination
T24 Phosphorylation
R28 Methylation
Y32 Phosphorylation
Y33 Phosphorylation
R37 Methylation
R57 Methylation
T70 Phosphorylation
S81 Phosphorylation
K85 Acetylation
K85 Sumoylation
K85 Ubiquitination
R90 Methylation
S95 Phosphorylation
R98 Methylation
S108 Phosphorylation
S120 Phosphorylation
S122 Phosphorylation
S124 Phosphorylation
S126 Phosphorylation
S128 Phosphorylation
S130 Phosphorylation
S138 Phosphorylation
S140 Phosphorylation
S148 Phosphorylation
S150 Phosphorylation
S152 Phosphorylation
S154 Phosphorylation

Research Backgrounds

Function:

Splicing factor that specifically promotes exon-inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. Also functions as export adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. RNA-binding is semi-sequence specific.

PTMs:

Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain.

Subcellular Location:

Nucleus. Nucleus speckle. Cytoplasm.
Note: Recruited to nuclear speckles following interaction with YTHDC1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CPSF6. Interacts with RBMY1A1. Interacts with SREK1/SFRS12. Interacts with NXF1. Interacts with YTHDC1, leading to recruitment to RNA elements adjacent to m6A sites.

Family&Domains:

Belongs to the splicing factor SR family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

References

1). SRSF3 facilitates replication of influenza A virus via binding and promoting the transport of viral mRNA. Veterinary Microbiology, 2022 (PubMed: 35063826) [IF=2.4]

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