Product: FOXK1 Antibody
Catalog: DF3231
Description: Rabbit polyclonal antibody to FOXK1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 78 KD; 75kD(Calculated).
Uniprot: P85037
RRID: AB_2835611

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
FOXK1 Antibody detects endogenous levels of total FOXK1.
Cite Format: Affinity Biosciences Cat# DF3231, RRID:AB_2835611.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


A630048H08Rik; AI463295; ENSMUSG00000075577; FHX; Fork head; Forkhead box protein K1; FOXJ2 forkhead factor; FOXK1; FOXK1_HUMAN; Gm10868; MNF; Myocyte nuclear factor;



Expressed both developing and adult tissues (PubMed:15289879). In adults, significant expression is seen in tumors of the brain, colon and lymph node (PubMed:15289879).




Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P85037 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S101 Phosphorylation
S122 Phosphorylation
R161 Methylation
K165 Ubiquitination
R177 Methylation
K186 Ubiquitination
R191 Methylation
T202 Phosphorylation
S203 Phosphorylation
Y205 Phosphorylation
S213 Phosphorylation
Y219 Phosphorylation
S223 Phosphorylation
K226 Ubiquitination
S236 Phosphorylation
S239 Phosphorylation
S243 Phosphorylation
T245 Phosphorylation
T247 Phosphorylation
S249 Phosphorylation
S253 Phosphorylation
S257 Phosphorylation
T273 Phosphorylation
K285 Ubiquitination
S288 Phosphorylation
T294 Phosphorylation
S295 Phosphorylation
S299 Phosphorylation
Y310 Phosphorylation
S329 Phosphorylation
Y339 Phosphorylation
Y341 Phosphorylation
Y342 Phosphorylation
K347 Ubiquitination
S352 Phosphorylation
R361 Methylation
K365 Ubiquitination
K375 Acetylation
K375 Sumoylation
K375 Ubiquitination
S377 Phosphorylation
K388 Ubiquitination
R400 Methylation
S403 Phosphorylation
C404 S-Nitrosylation
T407 Phosphorylation
S413 Phosphorylation
S416 Phosphorylation
S420 Phosphorylation
T422 Phosphorylation
S428 Phosphorylation
S431 Phosphorylation
T436 Phosphorylation
C439 S-Nitrosylation
S441 Phosphorylation
S445 Phosphorylation
S455 Phosphorylation
S459 Phosphorylation
Y465 Phosphorylation
S468 Phosphorylation
S472 Phosphorylation
S475 Phosphorylation
S644 Phosphorylation
T683 Phosphorylation
T686 Phosphorylation
S695 Phosphorylation
S696 Phosphorylation
T716 Phosphorylation
T731 Phosphorylation

Research Backgrounds


Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis, muscle cell differentiation and autophagy (By similarity). Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context. Together with FOXK2, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (By similarity). Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (By similarity). Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (By similarity). Involved in mTORC1-mediated metabolic reprogramming: in response to mTORC1 signaling, translocates into the nucleus and regulates the expression of genes associated with glycolysis and downstream anabolic pathways, such as HIF1A, thereby regulating glucose metabolism (By similarity). Together with FOXK2, acts as a negative regulator of autophagy in skeletal muscle: in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (By similarity). Acts as a transcriptional regulator of the myogenic progenitor cell population in skeletal muscle (By similarity). Binds to the upstream enhancer region (CCAC box) of myoglobin (MB) gene, regulating the myogenic progenitor cell population (By similarity). Promotes muscle progenitor cell proliferation by repressing the transcriptional activity of FOXO4, thereby inhibiting myogenic differentiation (By similarity). Involved in remodeling processes of adult muscles that occur in response to physiological stimuli (By similarity). Required to correct temporal orchestration of molecular and cellular events necessary for muscle repair (By similarity). Represses myogenic differentiation by inhibiting MEFC activity (By similarity). Positively regulates Wnt/beta-catenin signaling by translocating DVL into the nucleus. Reduces virus replication, probably by binding the interferon stimulated response element (ISRE) to promote antiviral gene expression.


Phosphorylation by GSK3 (GSK3A or GSK3B) promotes interaction with YWHAE/14-3-3-epsilon and retention in the cytoplasm. In response to mTORC1 signaling, phosphorylation by GSK3 is prevented, leading to translocation to the nucleus.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Translocation to the nucleus is regulated by phosphorylation: phosphorylation by GSK3 (GSK3A or GSK3B) promotes interaction with 14-3-3 proteins and sequestration in the cytoplasm. Dephosphorylation promotes translocation to the nucleus (By similarity). Accumulates in the nucleus upon viral infection (PubMed:25852164).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon and lymph node.

Subunit Structure:

Interacts with SIN3A and SIN3B (via PAH2) to form a complex which represses transcription (By similarity). Component of SIN3A-, but not SIN3B-, containing multiprotein complexes (By similarity). Interacts with FOXO4 and MEF2C; both interactions inhibit FOXO4 and MEF2C transactivation activity (By similarity). Interacts (when phosphorylated) with YWHAE/14-3-3-epsilon; promotes sequestration in the cytoplasm and leads to impaired ability to bind DNA (By similarity). Interacts with FHL2 (By similarity). Interacts with SRF. Interacts with DVL2 and DVL3; the interaction induces DVL2 nuclear translocation. Interacts with BAP1 (when phosphorylated).

Restrictive clause


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