ALKBH5 Recombinant Rabbit mAb - #BF4365

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Product: ALKBH5 Recombinant Rabbit mAb
Catalog: BF4365
Description: Rabbit monoclonal antibody to ALKBH5
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 44-48 kDa(Observed); 44kD(Calculated).
Uniprot: Q6P6C2

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000, IHC 1:200-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse, Rat
Clonality:
Monoclonal [ReFirm23815]
Specificity:
ALKBH5 Recombinant Rabbit mAb detects endogenous levels of ALKBH5.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABH5; AlkB, alkylation repair homolog 5 (E. coli); AlkB, alkylation repair homolog 5; Alkylated DNA repair protein alkB homolog 5; Alpha ketoglutarate dependent dioxygenase alkB homolog 5; OFOXD; OFOXD1; Oxoglutarate and iron-dependent oxygenase domain containing; Probable alpha ketoglutarate dependent dioxygenase ABH5; RNA demethylase ALKBH5;

Immunogens

Immunogen:

A synthetic peptide from human ALKBH5

Uniprot:

Q6P6C2(ALKB5_HUMAN) >>Visit HPA database.

Gene(ID):
ALKBH5(54890)
Expression:
Q6P6C2 ALKB5_HUMAN:

Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.

Description:
Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N6-methyladenosine (m6A) RNA,the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736,PubMed:24778178,PubMed:24616105,PubMed:24489119). Can also demethylate N6-methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Requires molecular oxygen,alpha-ketoglutarate and iron (PubMed:21264265,PubMed:23177736,PubMed:24778178,PubMed:24616105,PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis (By similarity).
Sequence:
MAAASGYTDLREKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGAKRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAGSPARKVKMRRH

Research Backgrounds

Function:

Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity).

Subcellular Location:

Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.

Family&Domains:

Belongs to the alkB family.

Restrictive clause

 

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