Product: CDKL1 Antibody
Catalog: DF3233
Description: Rabbit polyclonal antibody to CDKL1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 42 KD; 42kD(Calculated).
Uniprot: Q00532
RRID: AB_2835613

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Sheep(91%), Dog(82%)
Clonality:
Polyclonal
Specificity:
CDKL1 Antibody detects endogenous levels of total CDKL1.
RRID:
AB_2835613
Cite Format: Affinity Biosciences Cat# DF3233, RRID:AB_2835613.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDC 2 related kinase 1; CDC 2 related kinase; CDC2 related kinase 1; CDC2 related kinase; CDK L1; CDKL 1; cdkl1; CDKL1_HUMAN; Cyclin dependent kinase like 1; cyclin-dependent kinase-like 1 (CDC2-related kinase); Cyclin-dependent kinase-like 1; EC 2.7.11.22; KKIALRE; p42; Protein kinase p42 KKIALRE; Serine/threonine protein kinase KKIALRE; Serine/threonine-protein kinase KKIALRE;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q00532 CDKL1_HUMAN:

Highly expressed in kidney, and to a lower extent in ovary.

Sequence:
MMEKYEKIGKIGEGSYGVVFKCRNRDTGQIVAIKKFLESEDDPVIKKIALREIRMLKQLKHPNLVNLLEVFRRKRRLHLVFEYCDHTVLHELDRYQRGVPEHLVKSITWQTLQAVNFCHKHNCIHRDVKPENILITKHSVIKLCDFGFARLLAGPSDYYTDYVATRWYRSPELLVGDTQYGPPVDVWAIGCVFAELLSGVPLWPGKSDVDQLYLIRKTLGDLIPRHQQVFSTNQYFSGVKIPDPEDMEPLELKFPNISYPALGLLKGCLHMDPTQRLTCEQLLHHPYFENIREIEDLAKEHNKPTRKTLRKSRKHHCFTETSKLQYLPQLTGSSILPALDNKKYYCDTKKLNYRFPNI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Bovine
91
Sheep
91
Dog
82
Horse
64
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q00532 As Substrate

Site PTM Type Enzyme
K34 Ubiquitination

Research Backgrounds

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in kidney, and to a lower extent in ovary.

Family&Domains:

The [NKR]KIAxRE motif seems to be a cyclin-binding region.

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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